Profile

Annette T. Hastie, Ph.D.Wake Forest School of Medicine

Annette T. Hastie, Ph.D.

Associate Professor,

Contact Information

Academic: 336-713-7522 | Department: 336-713-7522

Email: ahastie@wakehealth.edu

Education & Training

  • A.B., Oberlin College, 1968
  • Ph.D., University of Pittsburgh, 1975
  • Fellowship, Sch Hyg & PH;Johns Hopkins U, 1979
  • Fellowship, Medicine, Temple University School of Me, 1984

Memberships

  • Sigma Xi Society
  • Am Soc Of Cell Biology
  • Am Soc of Microbiology
  • American Thoracic Society
Annette T. Hastie, Ph.D.

Annette T. Hastie, Ph.D.

Associate Professor, Center for Genomics and Personalized Medicine Research
Pulmonary, Critical Care, Allergy, and Immunologic Medicine

Research Interests

Asthma; Sputum; Eosinophils; Neutrophils; Cell Adhesion Molecules
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Contact Information

Academic: 336-713-7522 | Department: 336-713-7522

Email: ahastie@wakehealth.edu

Recent Publications

Expression of asthma susceptibility genes in bronchial epithelial cells and bronchial alveolar lavage in the Severe Asthma Research Program (SARP) cohort. Li X, Hawkins GA, Moore WC, Hastie AT, Ampleford EJ, Milosevic J, Li H, Busse WW, Erzurum SC, Kaminski N, Wenzel SE, Bleecker ER, Meyers DA.. J Asthma. 2016;53(8):775-782.

Plasma interleukin-6 concentrations, metabolic dysfunction, and asthma severity: a cross-sectional analysis of two cohorts. Peters MC, McGrath KW, Hawkins GA, Hastie AT, Levy BD, Israel E, Phillips BR, Mauger DT, Comhair SA, Erzurum SC, Johansson MW, Jarjour NN, Coverstone AM, Castro M, Holguin F, Wenzel SE, Woodruff PG, Bleecker ER, Fahy JV.. Lancet Respir Med. 2016;4(7):574-584.

Inflammatory and co-morbid features of patients with severe asthma and frequent exacerbations. Denlinger LC, Phillips BR, Ramratnam S, Ross K, Bhakta NR, Cardet JC, Castro M, Peters SP, Phipatanakul W, Aujla S, Bacharier LB, Bleecker ER, Comhair SA, Coverstone A, DeBoer M, Erzurum SC, Fain SB, Fajt M, Fitzpatrick AM, Gaffin J, Gaston B,.. Am J Respir Crit Care Med. 2016;():.

IL-6 trans-signaling increases expression of airways disease genes in airway smooth muscle. Robinson MB, Deshpande DA, Chou J, Cui W, Smith S, Langefeld C, Hastie AT, Bleecker ER, Hawkins GA.. Am J Physiol Lung Cell Mol Physiol. 2015;309(2):L129-L138.

eQTL of bronchial epithelial cells and bronchial alveolar lavage deciphers GWAS-identified asthma genes. Li X, Hastie AT, Hawkins GA, Moore WC, Ampleford EJ, Milosevic J, Li H, Busse WW, Erzurum SC, Kaminski N, Wenzel SE, Meyers DA, Bleecker ER.. Allergy. 2015;70(10):1309-1318.

Design of a multi-center immunophenotyping analysis of peripheral blood, sputum and bronchoalveolar lavage fluid in the Subpopulations and Intermediate Outcome Measures in COPD Study (SPIROMICS). Freeman CM, Crudgington S, Stolberg VR, Brown JP, Sonstein J, Alexis NE, Doerschuk CM, Basta PV, Carretta EE, Couper DJ, Hastie AT, Kaner RJ, O'Neal WK, Paine R III, Rennard SI, Shimbo D, Woodruff PG, Zeidler M, Curtis JL.. J Transl Med. 2015;13():19.

Analyses of COPD severity phenotypes and emphysema in spiromics subjects stratified By sputum granulocytes [abstract]. Hastie AT, Doerschuk CM, Alexis NE, Hansel NN, Peters SP, Barr RG, Couper D, Curtis JL, Han MK, Kanner RE, Kleerup EC, Martinez FJ, Putcha N, Rennard SI, Woodruff PG, Meyers DA, Bleecker ER.. Am J Respir Crit Care Med. 2015;191():A2861.

African ancestry is associated with lung function, healthcare utilization, and airways inflammation in African Americans with asthma [abstract]. Ortega VE, Moore WC, Zein J, Hastie AT, Ampleford E, Peters SP, Busse WW, Castro M, Erzurum SC, Israel E, Wenzel SE, Hawkins G, Bleecker ER, Meyers DA.. Am J Respir Crit Care Med. 2015;191():A1061.

Older asthma subjects are less atopic with increased sputum neutrophils [abstract]. Kaur R, Hastie AT, Smith R, Li H, Meyers DA, Bleecker ER, Moore WC.. Am J Respir Crit Care Med. 2015;191():A4324.

Effect of rare variants in ADRB2 on risk of severe exacerbations and symptom control during longacting beta agonist treatment in a multiethnic asthma population: a genetic study. Ortega VE, Hawkins GA, Moore WC, Hastie AT, Ampleford EJ, Busse WW, Castro M, Chardon D,Erzurum SC, Israel E, Montealegre F, Wezel SE, Peters SP, Meyers DA, Bleecker ER.. Lancet Respir Med. 2014;2(3):204-213.

Sputum neutrophil counts are associated with more severe asthma phenotypes using cluster analysis. Moore WC, Hastie AT, Li X, Li H, Busse WW, Jarjour NN, Wenzel SE, Peters SP, Meyers DA, Bleecker ER.. J Allergy Clin Immunol. 2014;133(6):1557-1563.

Defining fungal asthma severity via immune mediator differences in bronchoalveolar lavage fluid and induced sputum from fungal skin test-positive vs. skin test-negative asthmatics [abstract]. Steele C, Dunaway CW, Hastie AT, Ampleford EJ, Bleecker ER, Meyers DA.. Am J Respir Crit Care Med. 2014;189():A2176.

Serum soluble IL6 receptor levels are elevated in subjects with COPD and are associated with the IL6R coding variation Asp358Ala [abstract]. Hawkins G, Robinson MB, Moore WC, Hastie AT, Peters SP, Meyers DA, Bleecker ER, Berry M.. Am J Respir Crit Care Med. 2014;189():A4288.

Deciphering functional variants for genes associated with asthma susceptibility using eQTL of bronchial epithelial cells and bronchial alveolar lavage [abstract]. Li X, Moore WC, Hastie AT, Ampleford EJ, Li H, Hawkins GA, Busse WW, Erzurum SC, Kaminski N, Wenzel SE, Meyers DA, Bleecker ER.. Am J Respir Crit Care Med. 2014;189(Meeting Abstracts):A1001.

Complex patterns of Th1 inflammatory biomarker associations with cellular constituents of induced sputum in asthma [abstract]. Hastie AT, Dunaway CW, Moore WC, Foster S, Rector BM, Ampleford E, Bleecker ER, Steele C, Meyers DA.. Am J Respir Crit Care Med. 2014;189():A4236.

LIGHT is associated with increased cellular infiltrate and levels of TH1 cytokines as well as well as [sic] reduced lung function in human asthma [abstract]. Romeo J, Hastie AT, Foster S, Moore W, Peters SP, Bleecker ER, Dykewicz MS.. J Allergy Clin Immunol. 2013;131(2 Suppl):AB203.

Biomarker surrogates do not accurately predict sputum eosinophil and neutrophil percentages in asthmatic subjects. Hastie AT, Moore WC, Li H, Rector BM, Ortega VE, Pascual RM, Peters SP, Meyers DA, Bleecker ER.. J Allergy Clin Immunol. 2013;132(1):72-80.

Genome-wide association study identifies TH1 pathway genes associated with lung function in asthmatic patients. Li X, Hawkins GA, Ampleford EJ, Moore WC, Li H, Hastie AT, Howard TD, Boushey HA, Busse WW, Calhoun WJ, Castro M, Erzurum SC, Israel E, Lemanske RF Jr, Szefler SJ, Wasserman SI, Wenzel SE, Peters SP, Meyers DA, Bleecker ER.. J Allergy Clin Immunol. 2013;132(2):313-320.

Clinical heterogeneity in the severe asthma research program. Moore WC, Fitzpatrick AM, Li X, Hastie AT, Li H, Meyers DA, Bleecker ER.. Ann Am Thorac Soc. 2013;10(Suppl):S118-S124.

Serum and sputum interleukin-33 (IL-33) levels show no association with serum total IgE, positive skin test number, exhaled nitric oxide, or increasing severity of atopic asthma [abstract]. Hastie AT, Rector B, Moore WC, Li H, Peters SP, Meyers DA, Bleecker ER.. Am J Respir Crit Care Med. 2013;187():A1008.

Insulin like growth factor binding protein 2 (IGFBP2) is associated with increased sputum cellular infiltrate, increased sputum cytokine levels, and reduced lung function for human subjects with asthma [abstract]. Bowers B, Hastie AT, Rector B, Moore WC, Meyers DA, Peters SP, Bleecker ER.. Am J Respir Crit Care Med. 2013;187(Meeting Abstracts):A2397.

Phenotypic/genetic characteristics of atopic/non-atopic early/late onset asthma [abstract]. Li X, Moore WC, Ampleford EJ, Li H, Hawkins G, Hastie AT, Boushey HA, Busse WW, Castro M, Erzurum SC, Israel E, Lemanske RF, Szefler SJ, Wasserman SI, Wenzel SE, Peters SP, Meyers DA, Bleecker ER.. Am J Respir Crit Care Med. 2013;187(Meeting Abstracts):A2395.

Thioredoxin is positively associated with eosinophils in induced sputum from patients with asthma [abstract]. Hartman JM, Hastie A, Moore WC, Rector B, Peters SP, Bleecker ER, Dykewicz MS.. J Allergy Clin Immunol. 2012;129(2 Suppl):AB209.

Severe asthma: lessons learned from the National Heart, Lung, and Blood Institute Severe Asthma Research Program. Jarjour NN, Erzurum SC, Bleecker ER, Calhoun WJ, Castro M, Comhair SAA, Chung KF, Curran-Everett D, Dweik RA, Fain SB, Fitzpatrick AM, Gaston BM, Israel E, Hastie A, Hoffman EA, Holguin F, Levy BD, Meyers DA, Moore WC, Peters SP,.. Am J Respir Crit Care Med. 2012;185(4):356-362.

The addition of sputum inflammatory cell counts to the SARP cluster analysis results in similar clinical asthma phenotypes with biologic heterogeneity [abstract]. Moore WC, Hastie AT, Li H, Li X, Busse WW, Wenzel SE, Peters SP, Meyers DA, Bleecker ER.. Am J Respir Crit Care Med. 2012;185(Meeting Abstracts):A3625.

Effects of rare beta2-adrenergic receptor gene variants and long-acting beta agonists on healthcare utilization and corticosteroid use in two multi-ethnic asthma populations [abstract]. Ortega VE, Hawkins G, Hastie AT, Moore WC, Sadeghnejad A, Busse WW, Erzurum SC, Wenzel SE, Peters SP, Meyers DA, Bleecker ER.. Am J Respir Crit Care Med. 2012;185(Meeting Abstracts):A2514.

Blood eosinophils, exhaled nitric oxide, and IGE do not accurately predict sputum eosinophils [abstract]. Hastie AT, Moore WC, Li H, Rector BM, Pascual RM, Peters SP, Meyers DA, Bleecker ER.. Am J Respir Crit Care Med. 2012;185(Meeting Abstracts):A3926.

The IL6R variation Asp358 Ala is a potential modifier of lung function in subjects with asthma. Hawkins GA, Robinson MB, Hastie AT, Li X, Li H, Moore WC, Howard TD, Busse WW, Erzurum SC, Wenzel SE, Peters SP, Meyers DA, Bleecker ER.. J Allergy Clin Immunol. 2012;130(2):510-515.

Rare beta2-adrenergic receptor gene polymorphisms in asthma cases and controls from the Severe Asthma Research Program [abstract]. Ortega VE, Hastie A, Sadeghnejad A, Moore W, Hawkins GA, Peters SP, Busse WW, Calhoun WJ, Meyers DA, Bleecker ER, et al.. Am J Respir Crit Care Med. 2011;183(Meeting Abstracts):A1357.

Correlation of serum IL6 with lung function and phenotypic asthma clusters: data from the Severe Asthma Research Program (SARP) [abstract]. Hawkins GA, Robinson MB, Moore W, Hastie A, Pascual RM, Peters SP, Meyers DA, Bleecker ER.. Am J Respir Crit Care Med. 2011;183(Meeting Abstracts):A6169.

BAL cell lysate IL1b synthesis and processing correlates with blood eosinophil levels in subjects with asthma [abstract]. Hatzis CE, Hastie A, Moore W, Rector B, Sousa A, Knowles R, Peters SP, Meyers DA, Bleecker ER.. Am J Respir Crit Care Med. 2011;183(Meeting Abstracts):A4453.

Analyses of asthma severity phenotypes and inflammatory proteins in subjects stratified by sputum granulocytes. Hastie AT, Moore WC, Meyers DA, Vestal PL, Li H, Peters SP, Bleecker ER.. J Allergy Clin Immunol. 2010;125(5):1028-1036.

Inflammatory protein microarray analysis of sputum from obese subjects with asthma compared with nonobese subjects with asthma [abstract]. Hastie AT, Albon D, Moore WC, Krings J, Smith RV, Meyers DA, Peters SP, Bleecker ER.. Am J Respir Crit Care Med. 2010;181(1 Meeting Abstracts):A6801.

Increased numbers of macrophages and neutrophils are associated with decreased interleukin-33 (IL-33) levels in asthmatic sputum [abstract]. Bhatti SK, Hastie AT, Moore WC, Peters SP, Dykewicz MS, Bleecker ER.. J Allergy Clin Immunol. 2010;125(2 Suppl 1):AB109.

Serum levels of soluble IL6R are genetically regulated and correlate with lung function in asthmatics: Severe Asthma Research Program (SARP) [abstract]. Hawkins GA, Robinson MB, Moore WC, Hastie AT, Peters SP, Meyers DA, Bleecker ER.. J Allergy Clin Immunol. 2010;125(2 Suppl 1):AB357.

Inflammatory protein analyses of sputum from human subjects with severe or nonsevere asthma [abstract]. Hastie AT, Moore WC, Meyers DA, Vestal PL, Krings JO, Smith RA, Li H, Peters SP, Bleecker ER.. Am J Respir Crit Care Med. 2009;179(Online Abstr Issue):A3925.

Stem cell factor and cKit from asthmatic and normal subjects' epithelial cells and fibroblasts in response to inflammatory mediators [abstract]. Breit JS, Hastie AT, Vestal PL, Moore WC, Peters SP, Bleecker ER.. Am J Respir Crit Care Med. 2009;179(Online Abstr Issue):A5444.

Quantitative skin testing in asthmatic subjects enrolled in the Severe Asthma Research Program [abstract]. Laber DM, Hastie AT, Peters SP, Bleecker ER.. J Allergy Clin Immunol. 2009;123(3):730.

Surfactant alterations in asthmatics from the Wake Forest Severe Asthma Research Program Center [abstract]. Hite RD, Hastie AT, Seeds MC, Grier BL, Peters SP, Moore WC, Bleecker ER.. Am J Respir Crit Care Med. 2008;177(Abstr Issue):A100.

Genetic variation in pulmonary and activation-regulated chemokine (PARC/CCL18) in asthma [abstract]. Perry CD, Hawkins GA, Hastie AT, Cauthen CG, Diallo A, Li H, Moore WC, Meyers DA, Peters SP, Bleecker ER.. Am J Respir Crit Care Med. 2008;177(Online Abstr Issue):A338.

Collagen type I fragments associate with cytokines and cell counts in sputum of severe and nonsevere asthmatics [abstract]. Cauthen CG, Hastie AT, Wu M, Vestal PL, Moore WC, Krings J, Smith RV, Bleecker ER, Peters SP.. Am J Respir Crit Care Med. 2008;177(Online Abstr Issue):A813.

Proteomic microarray analyses of sputum from subjects with asthma compared to subjects with chronic obstructive pulmonary disease (COPD) [abstract]. Hastie AT, Vestal PL, Moore WC, Krings J, Smith RV, Peters SP, Bleecker ER.. Am J Respir Crit Care Med. 2008;177(Online Abstr Issue):A960.

Corticosteroid effect on brain derived neurotrophic factor (BDNF) in serum and sputum of asthmatics in vivo, and in asthmatic sputum cells and airway fibroblasts in vitro [abstract]. Surka AE, Hastie AT, Moore WC, Wu M, Krings J, Smith R, Peters SP, Bleecker ER.. Am J Respir Crit Care Med. 2007;175(Abstr Issue):A56.

Relationship of sputum cells with pulmonary function, symptoms and health-care utilization as a function of asthma severity [abstract]. Hastie AT, Wenzel SE, Chung KF, Busse WW, Bleecker ER.. Am J Respir Crit Care Med. 2007;175(Abstr Issue):A153.

Association of polymorphisms in the tumor necrosis factor-alpha (TNFalpha) gene with levels of TNFalpha and pulmonary function in asthma [abstract]. McCallister JW, Moore WC, Hastie AT, Vestal PL, Ampleford EJ, Hawkins GA, Wenzel SE, Meyers DA, Bleecker ER.. Am J Respir Crit Care Med. 2007;175(Abstr Issue):A462.

Levels of tumor necrosis factor alpha (TNFa) and brain derived neurotrophic factor (BDNF) correlate in sputum but not in serum [abstract]. Surka AE, McCallister JW, Hastie AT, Moore WC, Vestel [sic] PL [Vestal PL], Krings J, Smith R, Peters SP, Bleecker ER.. Am J Respir Crit Care Med. 2007;175(Abstr Issue):A681.

Lack of correlation between sputum, exhaled breath condensate and serum levels of tumor necrosis factor-alpha (TNFalpha) in asthma [abstract]. McCallister JW, Hastie AT, Moore WC, Vestal PL, Krings JO, Smith RV, Peters SP, Bleecker ER.. Am J Respir Crit Care Med. 2007;175(Abstr Issue):A684.

All Publications

For a listing of recent publications, refer to PubMed, a service provided by the National Library of Medicine.

For a list of earlier publications, visit the Carpenter Library Publication Search.

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