Douglas S. Lyles, Ph.D.Wake Forest School of Medicine

Douglas S. Lyles, Ph.D.

Program Director,

Contact Information

Department: 336-716-4237


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Education & Training

  • B.A., University of Pennsylvania, 1972
  • Ph.D., University of Mississippi School of Medicine, 1975
  • Fellowship, Rockefeller University, 1978


  • Am Soc Of Biochem & Mol Biolog
  • Am Soc Of Virology
  • Am Soc of Microbiology
Douglas S. Lyles, Ph.D.

Douglas S. Lyles, Ph.D.

Program Director, Comprehensive Cancer Center
Chair, Biochemistry
Professor, Biochemistry
Center for Comparative Medicine Research
Comprehensive Cancer Center

Research Interests

cancer/oncogenesis, cell growth, differentiation,d, genetics/genome, immunology/allergy/inflammatio, infectious diseases, molecular biology/molecular me, structural biology, vaccines

Contact Information

Department: 336-716-4237


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Recent Publications

Yacovone SK, Ornelles DA, Lyles DS. The border-to-border distribution method for analysis of cytoplasmic particles and organelles. Cell Tissue Res. 2016;363(2):351-360.

Yacovone SK, Smelser AM, Macosko JC, Holzwarth G, Ornelles DA, Lyles DS. Migration of nucleocapsids in vesicular stomatitis virus-infected cells is dependent on both microtubules and actin filaments. J Virol. 2016;90(13):6159-6170.

Yu N, Puckett S, Antinozzi PA, Cramer SD, Lyles DS. Changes in susceptibility to oncolytic vesicular stomatitis virus during progression of prostate cancer. J Virol. 2015;89(10):5250-5263.

Westcott MM, Liu J, Rajani K, D'Agostino R Jr, Lyles DS, Porosnicu M. Interferon beta and interferon alpha 2a differentially protect head and neck cancer cells from vesicular stomatitis virus-induced oncolysis. J Virol. 2015;89(15):7944-7954.

Davidson A, Lyles D, Alexander R. RNA-seq reveals differential hnRNP-RNA interactions during VSV infection [abstract]. FASEB J. 2015;29(Suppl 1):718.22.

Smedberg JR, Westcott MM, Ahmed M, Lyles DS. Signaling pathways in murine dendritic cells that regulate the response to vesicular stomatitis virus vectors that express flagellin. J Virol. 2014;88(2):777-785.

Blackham AU, Northrup SA, Willingham M, Sirintrapun J, Russell GB, Lyles DS, Stewart JH IV. Molecular determinants of susceptibility to oncolytic vesicular stomatitis virus in pancreatic adenocarcinoma. J Surg Res. 2014;187(2):412-426.

Blackham AU, Northrup SA, Willingham M, D'Agostino RB Jr, Lyles DS, Stewart JH IV. Variation in susceptibility of human malignant melanomas to oncolytic vesicular stomatitis virus. Surgery. 2013;153(3):333-343.

Lyles DS. Assembly and budding of negative-strand RNA viruses. Adv Virus Res. 2013;85():57-90.

Lawrence TM, Wanjalla CN, Gomme EA, Wirblich C, Gatt A, Carnero E, Garcia-Sastre A, Lyles DS, McGettigan JP, Schnell MJ. Comparison of heterologous prime-boost strategies against human immunodeficiency virus type 1 Gag using negative stranded RNA viruses. PLoS One. 2013;8(6):e67123.

Westcott MM, Ahmed M, Smedberg JR, Rajani KR, Hiltbold EM, Lyles DS. Preservation of dendritic cell function during vesicular stomatitis virus infection reflects both intrinsic and acquired mechanisms of resistance to suppression of host gene expression by viral M protein. J Virol. 2013;87(21):11730-40.

Randle RW, Northrup SA, Sirintrapun SJ, Lyles DS, Stewart JH. Oncolytic vesicular stomatitis virus as a treatment for neuroendocrine tumors. Surgery. 2013;154(6):1323-1330.

Rajani KR, Pettit Kneller EL, McKenzie MO, Horita DA, Chou JW, Lyles DS. Complexes of vesicular stomatitis virus matrix protein with host Rae1 and Nup98 involved in inhibition of host transcription. PLoS Pathog. 2012;8(9):e1002929.

Johnson JB, Lyles DS, Alexander-Miller MA, Parks GD. Virion-associated complement regulator CD55 is more potent than CD46 in mediating resistance of mumps virus and vesicular stomatitis virus to neutralization. J Virol. 2012;86(18):9929-9940.

Johnson JB, Douglas LS [sic] [Lyles DS], Martha AMA [sic] [Alexander-Miller MA], Griffith DG [sic] [Parks GD]. Virion-associated complement regulator CD55 is more potent than CD46 in mediating resistance of mumps virus and VSV to neutralization [abstract]. Immunobiology. 2012;217(11):1215-1216.

Cary ZD, Willingham MC, Lyles DS. Oncolytic vesicular stomatitis virus induces apoptosis in U87 glioblastoma cells by a type II death receptor mechanism and induces cell death and tumor clearance in vivo. J Virol. 2011;85(12):5708-5717.

Gerlier D, Lyles DS. Interplay between innate immunity and negative-strand RNA viruses: towards a rational model. Microbiol Mol Biol Rev. 2011;75(3):468-490.

Stewart JH IV, Ahmed M, Northrup SA, Willingham M, Lyles DS. Vesicular stomatitis virus as a treatment for colorectal cancer. Cancer Gene Ther. 2011;18(12):837-849.

Braxton CL, Puckett SH, Mizel SB, Lyles DS. Protection against lethal vaccinia virus challenge by using an attenuated matrix protein mutant vesicular stomatitis virus vaccine vector expressing poxvirus antigens. J Virol. 2010;84(7):3552-3561.

Johnson JB, Lyles DS, Parks GD. Complement evasion by vesicular stomatitis virus involves recruitment of host complement regulatory proteins [abstract]. Mol Immunol. 2010;47(13):2239.

Porosnicu M, Liu J, Lyles D. Susceptibility of head and neck cancer to an attenuated model of the oncolytic vesicular stomatitis virus expressing suicide genes [abstract]. J Clin Oncol. 2010;27(15 Suppl):e14604.

Ahmed M, Puckett S, Arimilli S, Braxton CL, Mizel SB, Lyles DS. Stimulation of human dendritic cells by wild-type and M protein mutant vesicular stomatitis viruses engineered to express bacterial flagellin. J Virol. 2010;84(22):12093-98.

Ahmed M, Puckett S, Lyles DS. Susceptibility of breast cancer cells to an oncolytic matrix (M) protein mutant of vesicular stomatitis virus. Cancer Gene Ther. 2010;17(12):883-892.

Dancho B, McKenzie MO, Connor JH, Lyles DS. Vesicular stomatitis virus matrix protein mutations that affect association with host membranes and viral nucleocapsids. J Biol Chem. 2009;284(7):4500-4509.

Kneller ELP, Connor JH, Lyles DS. hnRNPs relocalize to the cytoplasm following infection with vesicular stomatitis virus. J Virol. 2009;83(2):770-780.

Ahmed M, Mitchell LM, Puckett S, Brzoza-Lewis KL, Lyles DS, Hiltbold EM. Vesicular stomatitis virus M protein mutant stimulates maturation of Toll-like receptor 7 (TLR7)-positive dendritic cells through TLR-dependent and -independent mechanisms. J Virol. 2009;83(7):2962-2975.

Agnihothram SS, Dancho B, Grant KW, Grimes ML, Lyles DS, Nunberg JH. Assembly of arenavirus envelope glycoprotein GPC in detergent-soluble membrane microdomains. J Virol. 2009;83(19):9890-9900.

Pearce AF, Lyles DS. Vesicular stomatitis virus induces apoptosis primarily through Bak rather than Bax by inactivating Mcl-1 and Bcl- XL. J Virol. 2009;83(18):9102-9112.

Faul EJ, Lyles DS, Schnell MJ. Interferon response and viral evasion by members of the family Rhabdoviridae. Viruses. 2009;1(3):832-851.

Whitlow ZW, Connor JH, Lyles DS. New mRNAs are preferentially translated during vesicular stomatitis virus infection. J Virol. 2008;82(5):2286-2294.

Swinteck BD, Lyles DS. Plasma membrane microdomains containing vesicular stomatitis virus M protein are separate from microdomains containing G protein and nucleocapsids. J Virol. 2008;82(11):5536-5547.

Ahmed M, Marino TR, Puckett S, Kock ND, Lyles DS. Immune response in the absence of neurovirulence in mice infected with m protein mutant vesicular stomatitis virus. J Virol. 2008;82(18):9273-9277.

Carey BL, Ahmed M, Puckett S, Lyles DS. Early steps of the virus replication cycle are inhibited in prostate cancer cells resistant to oncolytic vesicular stomatitis virus. J Virol. 2008;82(24):12104-15.

Lyles DS, Rupprecht CE. Rhabdoviridae In: Knipe DM, Howley PM, Griffin DE, Lamb RA, Straus SE, Martin MA, Roizman B, eds. Fields virology. 5th ed. Philadelphia: Wolters Kluwer/Lippincott Williams & Wilkins;2007: 1363-1408.

Gaddy DF, Lyles DS. Oncolytic vesicular stomatitis virus induces apoptosis via signaling through PKR, Fas, and Daxx. J Virol. 2007;81(6):2792-2804.

Connor JH, McKenzie MO, Parks GD, Lyles DS. Antiviral activity and RNA polymerase degradation following Hsp90 inhibition in a range of negative strand viruses. Virology. 2007;362(1):109-119.

Trottier MD, Lyles DS, Reiss CS. Peripheral, but not central nervous system, type I interferon expression in mice in response to intranasal vesicular stomatitis virus infection. J Neurovirol. 2007;13(5):433-445.

Whitlow ZW, Connor JH, Lyles DS. Preferential translation of vesicular stomatitis virus mRNAs is conferred by transcription from the viral genome. J Virol. 2006;80(23):11733-42.

All Publications

For a listing of recent publications, refer to PubMed, a service provided by the National Library of Medicine.

For a list of earlier publications, visit the Carpenter Library Publication Search.

Professor, Biochemistry

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