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Douglas S. Lyles, Ph.D.Wake Forest School of Medicine

Douglas S. Lyles, Ph.D.

Program Director,
Chair,
Professor,
Ctr for Vaccines at Extremes

Contact Information

Department: 336-716-4237

Email: dlyles@wakehealth.edu

Related Links

Education & Training

  • B.A., University of Pennsylvania, 1972
  • Ph.D., University of Mississippi School of Medicine, 1975
  • Fellowship, Rockefeller University, 1978

Memberships

  • Am Soc Of Biochem & Mol Biolog
  • Am Soc of Microbiology
  • Am Soc Of Virology
Douglas S. Lyles, Ph.D.

Douglas S. Lyles, Ph.D.

Program Director, Comprehensive Cancer Center
Chair, Biochemistry
Professor, Biochemistry
Ctr for Vaccines at Extremes

Research Interests

Vesicular stomatitis Indiana virus; Viral Matrix Proteins; Vesiculovirus; Apoptosis; Virus Replication
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Contact Information

Department: 336-716-4237

Email: dlyles@wakehealth.edu

Related Links

Recent Publications

The border-to-border distribution method for analysis of cytoplasmic particles and organelles. Yacovone SK, Ornelles DA, Lyles DS.. Cell Tissue Res. 2016;363(2):351-360.

Migration of nucleocapsids in vesicular stomatitis virus-infected cells is dependent on both microtubules and actin filaments. Yacovone SK, Smelser AM, Macosko JC, Holzwarth G, Ornelles DA, Lyles DS.. J Virol. 2016;90(13):6159-6170.

Recruitment of CD46 and CD55 by Vesicular Stomatitis Virus is a specific modulatory function adopted by the virus to evade complement mediated neutralization [abstract]. Kumar NA, Lyles DS, Johnson JB.. Immunobiology. 2016;221(10):1216.

Changes in susceptibility to oncolytic vesicular stomatitis virus during progression of prostate cancer. Yu N, Puckett S, Antinozzi PA, Cramer SD, Lyles DS.. J Virol. 2015;89(10):5250-5263.

Interferon beta and interferon alpha 2a differentially protect head and neck cancer cells from vesicular stomatitis virus-induced oncolysis. Westcott MM, Liu J, Rajani K, D'Agostino R Jr, Lyles DS, Porosnicu M.. J Virol. 2015;89(15):7944-7954.

RNA-seq reveals differential hnRNP-RNA interactions during VSV infection [abstract]. Davidson A, Lyles D, Alexander R.. FASEB J. 2015;29(Suppl 1):718.22.

Signaling pathways in murine dendritic cells that regulate the response to vesicular stomatitis virus vectors that express flagellin. Smedberg JR, Westcott MM, Ahmed M, Lyles DS.. J Virol. 2014;88(2):777-785.

Molecular determinants of susceptibility to oncolytic vesicular stomatitis virus in pancreatic adenocarcinoma. Blackham AU, Northrup SA, Willingham M, Sirintrapun J, Russell GB, Lyles DS, Stewart JH IV.. J Surg Res. 2014;187(2):412-426.

Variation in susceptibility of human malignant melanomas to oncolytic vesicular stomatitis virus. Blackham AU, Northrup SA, Willingham M, D'Agostino RB Jr, Lyles DS, Stewart JH IV.. Surgery. 2013;153(3):333-343.

Assembly and budding of negative-strand RNA viruses. Lyles DS.. Adv Virus Res. 2013;85():57-90.

Comparison of heterologous prime-boost strategies against human immunodeficiency virus type 1 Gag using negative stranded RNA viruses. Lawrence TM, Wanjalla CN, Gomme EA, Wirblich C, Gatt A, Carnero E, Garcia-Sastre A, Lyles DS, McGettigan JP, Schnell MJ.. PLoS One. 2013;8(6):e67123.

Preservation of dendritic cell function during vesicular stomatitis virus infection reflects both intrinsic and acquired mechanisms of resistance to suppression of host gene expression by viral M protein. Westcott MM, Ahmed M, Smedberg JR, Rajani KR, Hiltbold EM, Lyles DS.. J Virol. 2013;87(21):11730-40.

Oncolytic vesicular stomatitis virus as a treatment for neuroendocrine tumors. Randle RW, Northrup SA, Sirintrapun SJ, Lyles DS, Stewart JH.. Surgery. 2013;154(6):1323-1330.

Complexes of vesicular stomatitis virus matrix protein with host Rae1 and Nup98 involved in inhibition of host transcription. Rajani KR, Pettit Kneller EL, McKenzie MO, Horita DA, Chou JW, Lyles DS.. PLoS Pathog. 2012;8(9):e1002929.

Virion-associated complement regulator CD55 is more potent than CD46 in mediating resistance of mumps virus and vesicular stomatitis virus to neutralization. Johnson JB, Lyles DS, Alexander-Miller MA, Parks GD.. J Virol. 2012;86(18):9929-9940.

Virion-associated complement regulator CD55 is more potent than CD46 in mediating resistance of mumps virus and VSV to neutralization [abstract]. Johnson JB, Douglas LS [sic] [Lyles DS], Martha AMA [sic] [Alexander-Miller MA], Griffith DG [sic] [Parks GD].. Immunobiology. 2012;217(11):1215-1216.

Oncolytic vesicular stomatitis virus induces apoptosis in U87 glioblastoma cells by a type II death receptor mechanism and induces cell death and tumor clearance in vivo. Cary ZD, Willingham MC, Lyles DS.. J Virol. 2011;85(12):5708-5717.

Interplay between innate immunity and negative-strand RNA viruses: towards a rational model. Gerlier D, Lyles DS.. Microbiol Mol Biol Rev. 2011;75(3):468-490.

Vesicular stomatitis virus as a treatment for colorectal cancer. Stewart JH IV, Ahmed M, Northrup SA, Willingham M, Lyles DS.. Cancer Gene Ther. 2011;18(12):837-849.

Protection against lethal vaccinia virus challenge by using an attenuated matrix protein mutant vesicular stomatitis virus vaccine vector expressing poxvirus antigens. Braxton CL, Puckett SH, Mizel SB, Lyles DS.. J Virol. 2010;84(7):3552-3561.

Complement evasion by vesicular stomatitis virus involves recruitment of host complement regulatory proteins [abstract]. Johnson JB, Lyles DS, Parks GD.. Mol Immunol. 2010;47(13):2239.

Susceptibility of head and neck cancer to an attenuated model of the oncolytic vesicular stomatitis virus expressing suicide genes [abstract]. Porosnicu M, Liu J, Lyles D.. J Clin Oncol. 2010;27(15 Suppl):e14604.

Stimulation of human dendritic cells by wild-type and M protein mutant vesicular stomatitis viruses engineered to express bacterial flagellin. Ahmed M, Puckett S, Arimilli S, Braxton CL, Mizel SB, Lyles DS.. J Virol. 2010;84(22):12093-98.

Susceptibility of breast cancer cells to an oncolytic matrix (M) protein mutant of vesicular stomatitis virus. Ahmed M, Puckett S, Lyles DS.. Cancer Gene Ther. 2010;17(12):883-892.

Vesicular stomatitis virus matrix protein mutations that affect association with host membranes and viral nucleocapsids. Dancho B, McKenzie MO, Connor JH, Lyles DS.. J Biol Chem. 2009;284(7):4500-4509.

hnRNPs relocalize to the cytoplasm following infection with vesicular stomatitis virus. Kneller ELP, Connor JH, Lyles DS.. J Virol. 2009;83(2):770-780.

Vesicular stomatitis virus M protein mutant stimulates maturation of Toll-like receptor 7 (TLR7)-positive dendritic cells through TLR-dependent and -independent mechanisms. Ahmed M, Mitchell LM, Puckett S, Brzoza-Lewis KL, Lyles DS, Hiltbold EM.. J Virol. 2009;83(7):2962-2975.

Assembly of arenavirus envelope glycoprotein GPC in detergent-soluble membrane microdomains. Agnihothram SS, Dancho B, Grant KW, Grimes ML, Lyles DS, Nunberg JH.. J Virol. 2009;83(19):9890-9900.

Vesicular stomatitis virus induces apoptosis primarily through Bak rather than Bax by inactivating Mcl-1 and Bcl- XL. Pearce AF, Lyles DS.. J Virol. 2009;83(18):9102-9112.

Interferon response and viral evasion by members of the family Rhabdoviridae. Faul EJ, Lyles DS, Schnell MJ.. Viruses. 2009;1(3):832-851.

New mRNAs are preferentially translated during vesicular stomatitis virus infection. Whitlow ZW, Connor JH, Lyles DS.. J Virol. 2008;82(5):2286-2294.

Plasma membrane microdomains containing vesicular stomatitis virus M protein are separate from microdomains containing G protein and nucleocapsids. Swinteck BD, Lyles DS.. J Virol. 2008;82(11):5536-5547.

Immune response in the absence of neurovirulence in mice infected with m protein mutant vesicular stomatitis virus. Ahmed M, Marino TR, Puckett S, Kock ND, Lyles DS.. J Virol. 2008;82(18):9273-9277.

Early steps of the virus replication cycle are inhibited in prostate cancer cells resistant to oncolytic vesicular stomatitis virus. Carey BL, Ahmed M, Puckett S, Lyles DS.. J Virol. 2008;82(24):12104-15.

Rhabdoviridae. Lyles DS, Rupprecht CE. In: Knipe DM, Howley PM, Griffin DE, Lamb RA, Straus SE, Martin MA, Roizman B, eds. Fields virology. 5th ed. Philadelphia: Wolters Kluwer/Lippincott Williams & Wilkins;2007: 1363-1408.

Oncolytic vesicular stomatitis virus induces apoptosis via signaling through PKR, Fas, and Daxx. Gaddy DF, Lyles DS.. J Virol. 2007;81(6):2792-2804.

Antiviral activity and RNA polymerase degradation following Hsp90 inhibition in a range of negative strand viruses. Connor JH, McKenzie MO, Parks GD, Lyles DS.. Virology. 2007;362(1):109-119.

Peripheral, but not central nervous system, type I interferon expression in mice in response to intranasal vesicular stomatitis virus infection. Trottier MD, Lyles DS, Reiss CS.. J Neurovirol. 2007;13(5):433-445.

Preferential translation of vesicular stomatitis virus mRNAs is conferred by transcription from the viral genome. Whitlow ZW, Connor JH, Lyles DS.. J Virol. 2006;80(23):11733-42.

All Publications

For a listing of recent publications, refer to PubMed, a service provided by the National Library of Medicine.

For a list of earlier publications, visit the Carpenter Library Publication Search.

Professor, Biochemistry

Douglas S. Lyles, Ph.D.

Douglas S. Lyles, Ph.D.

Program Director, Comprehensive Cancer Center
Chair, Biochemistry
Professor, Biochemistry
Ctr for Vaccines at Extremes

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