Profile

Emmanuel C. Opara, Ph.D.Wake Forest School of Medicine

Emmanuel C. Opara, Ph.D.

Professor,


Contact Information

Academic: 336-713-1297 | Department: 336-716-2011

Email: eopara@wakehealth.edu

Education & Training

  • Master's Degree, University of Surrey-United Kingdom, 1980
  • Ph.D., London Hospital Medical Coll-United Kingdom, 1983
  • Fellowship, Endocrinology, Mayo Clinic School of Medicine, 1986
Emmanuel C. Opara, Ph.D.

Emmanuel C. Opara, Ph.D.

Professor, Institute for Regenerative Medicine
Center on Diabetes, Obesity, and Metabolism
Biomedical Engineering

Research Interests

Islets of Langerhans Transplantation; Alginates; Islets of Langerhans; Antioxidants; Pancreas, Artificial
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Contact Information

Academic: 336-713-1297 | Department: 336-716-2011

Email: eopara@wakehealth.edu

Recent Publications

Determination of the mechanical strength of microcapsules. Darabbie MD, Opara EC.. Methods Mol Biol. 2017;1479():111-118.

Selective osmotic shock (SOS)-based islet isolation for microencapsulation. Enck K, McQuilling JP, Orlando G, Tamburrini R, Sivanandane S, Opara EC.. Methods Mol Biol. 2017;1479():191-198.

A method of porcine pancreatic islet isolation for microencapsulation. Kendall WF Jr, Opara EC.. Methods Mol Biol. 2017;1479():175-189.

Polymeric materials for perm-selective coating of alginate microbeads. Kendall WF Jr, Opara EC.. Methods Mol Biol. 2017;1479():95-109.

Methods for incorporating oxygen-generating biomaterials into cell culture and microcapsule systems. McQuilling JP, Opara EC.. Methods Mol Biol. 2017;1479():135-141.

Applications of cell microencapsulation. Opara EC.. Methods Mol Biol. 2017;1479():23-39.

Microfluidic approach to cell microencapsulation. Sharma V, Hunckler M, Ramasubramanian MK, Opara EC, Katuri KC.. Methods Mol Biol. 2017;1479():71-76.

Compartmentalization of two cell types in multilayered alginate microcapsules. Sittadjody S, Saul JM, Opara EC.. Methods Mol Biol. 2017;1479():225-235.

Retrieval of microencapsulated islet grafts for post-transplant evaluation. McQuilling JP, Sittadjody S, Pareta R, Pendergraft S, Clark CJ, Farney AC, Opara EC.. Methods Mol Biol. 2017;1479():157-171.

Evolution of islet transplantation for the last 30 years. Farney AC, Sutherland DE, Opara EC.. Pancreas. 2016;45(1):8-20.

The human pancreas as a source of protolerogenic extracellular matrix scaffold for a new-generation bioartificial endocrine pancreas. Peloso A, Urbani L, Cravedi P, Katari R, Maghsoudlou P, Fallas ME, Sordi V, Citro A, Purroy C, Niu G, McQuilling JP, Sittadjody S, Farney AC, Iskandar SS, Zambon JP, Rogers J, Stratta RJ, Opara EC, Piemonti L, Furdui CM, Soker S, De Coppi P, Orlando G.. Ann Surg. 2016;264(1):169-179.

The combined effect of PDX1, epidermal growth factor and poly-L-ornithine on human amnion epithelial cells' differentiation. Balaji S, Zhou Y, Ganguly A, Opara EC, Soker S.. BMC Dev Biol. 2016;16():8.

Evaluation of the tissue response to alginate encapsulated islets in an omentum pouch model. Ibarra V, Appel AA, Anastasio MA, Opara EC, Brey EM.. J Biomed Mater Res A. 2016;104(7):1581-1590.

Encapsulated cells for hormone replacement therapy. Opara E, Yoo J, Justin S, Sivanandane S, Atala A, inventors; Wake Forest University Health Sciences, assignee.. United States patent US 9,283,251. 2016 Mar 15. 2016;():.

This paper is a winner in the Undergraduate category for the SFB awards: evaluation of the tissue response to alginate encapsulated islets in an omentum pouch model. Ibarra V, Appel AA, Anastasio MA, Opara EC, Brey EM.. J Biomed Mater Res A. 2016;104(7):1581-1590.

Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct. Yang Y, Opara EC, Liu Y, Atala A, Zhao W.. Exp Biol Med (Maywood). 2016;():.

Imaging of hydrogel microsphere structure and foreign body response based on endogenous X-ray phase contrast. Appel AA, Ibarra V, Somo SI, Larson JC, Garson AB III, Guan H, McQuilling JP, Zhong Z, Anastasio MA, Opara EC, Brey EM.. Tissue Eng Part C Methods. 2016;22(11):1038-1048.

Skeletal myogenic differentiation of urine-derived stem cells using hydrogel loaded with growth factors for potential treatment of urinary incontinence [abstract]. Jiang Y, Yi H, Liu G, Atala A, Opara EC, Zhang Y.. Tissue Eng Part A. 2015;21(Suppl 1):S248.

The effect of substrate elasticity on the function of ovarian follicles [abstract]. Joo S, Oh S, Sivanandane S, Opara E, Jackson J, Lee S, Yoo J, Atala A.. Tissue Eng Part A. 2015;21(Suppl 1):S30.

Development of techniques for pancreatic beta-cell engineering [abstract]. Niu G, McQuilling J, Peloso A, Opara EC, Orlando G, Soker S.. Tissue Eng Part A. 2015;21(Suppl 1):S289.

The human pancreas as a source of pro-tolerogenic extracellular matrix scaffold for a new generation bio-artificial endocrine pancreas [abstract]. Peloso A, Orlando G, Urbani L, Cravedi P, Sordi V, Guoguang N, Citro A, Mcquilling J, Farney A, Iskandar S, Rogers J, Stratta R, Opara E, Piemonti L, Soker S, De Coppi P, Petrosyan A.. Tissue Eng Part A. 2015;21(Suppl 1):S141.

The human pancreas as a source of protolerogenic extracellular matrix scaffold for a new generation bioartificial endocrine pancreas [abstract]. Orlando G, Peloso A, Urbani L, Cravedi P, Katari R, Maghsoudlou P, Fallas EMA, Sordi V, Purroy C, Niu GG, Mcquilling JP, Sivanandane S, Farney AC, Iskandar SS, Rogers J, Stratta RJ, Opara EC, Piemonti L, Soker S, De Coppi P.. Transpl Int. 2015;28(Suppl 4):129.

Scientific principles of regenerative medicine and their application in the female reproductive system. Yalcinkaya TM, Sittadjody S, Opara EC.. Maturitas. 2014;77(1):12-19.

Effects of allogeneic bone marrow derived mesenchymal stromal cell therapy on voiding function in a rat model of Parkinson disease. Campeau L, Soler R, Sittadjody S, Pareta R, Nomiya M, Zarifpour M, Opara EC, Yoo JJ, Andersson KE.. J Urol. 2014;191(3):850-859.

Long-term function of islets encapsulated in a redesigned alginate microcapsule construct in omentum pouches of immune-competent diabetic rats. Pareta R, McQuilling JP, Sittadjody S, Jenkins R, Bowden S, Orlando G, Farney AC, Brey EM, Opara EC.. Pancreas. 2014;43(4):605-613.

X-ray phase contrast imaging of encapsulated cells and foreign body response [abstract]. Appel AA, Ibarra V, Garson AB III, Guan H, Zhong Z, Anastasio MA, Opara EC, Brey EM.. Tissue Eng Part A. 2014;20(Suppl 1):S10.

Evaluation and control of alginate microbead stability for islet encapsulation [abstract]. Ibarra VA, Appel AA, Somo SI, Cheng M, Kao S, Anastasio MA, Garson A, Opara EC, Khanna O, Brey EM.. Tissue Eng Part A. 2014;20(Suppl 1):S23.

Presence of bone marrow stem cells increases estrogen production of ovarian cells for cell-based hormone replacement therapy in vitro [abstract]. Wells AK, Sittadjody S, Yoo JJ, Opara EC.. Tissue Eng Part A. 2014;20(Suppl 1):S139.

Vascularization of encapsulated cells. Balaji S, McQuilling JP, Khanna O, Brey EM, Opara EC. In: Brey EM, ed. Vascularization regenerative medicine and tissue engineering. Hobokin: Taylor and Francis;2014: 283-300.

Engineered multilayer ovarian tissue that secretes sex steroids and peptide hormones in response to gonadotropins. Sittadjody S, Saul JM, Joo S, Yoo JJ, Atala A, Opara EC.. Biomaterials. 2013;34(10):2412-2420.

Skeletal myogenic differentiation of urine-derived stem cells and angiogenesis using microbeads loaded with growth factors. Liu G, Pareta RA, Wu R, Shi Y, Zhou X, Liu H, Deng C, Sun X, Atala A, Opara EC, Zhang Y.. Biomaterials. 2013;34(4):1311-1326.

Microencapsulation of pancreatic islets for use in a bioartificial pancreas. Opara EC, McQuilling JP, Farney AC.. Methods Mol Biol. 2013;1001():261-266.

Design of a bioartificial pancreas. Pareta RA, Farney AC, Opara EC.. Pathobiology. 2013;80(4):194-202.

Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering. Mirmalek-Sani SH, Orlando G, McQuilling JP, Pareta R, Mack DL, Salvatori M, Farney AC, Stratta RJ, Atala A, Opara EC, Soker S.. Biomaterials. 2013;34(22):5488-5495.

Novel 3D co-culture model for epithelial-stromal cells interaction in prostate cancer. Fang X, Sittadjody S, Gyabaah K, Opara EC, Balaji KC.. PLoS One. 2013;8(9):e75187.

FGF-1 delivery from multilayer alginate microbeads stimulates a rapid and persistent increase in vascular density. Khanna O, Huang JJ, Moya ML, Wu CW, Cheng MH, Opara EC, Brey EM.. Microvasc Res. 2013;90():23-29.

Stability of alginate microbead properties in vitro. Moya ML, Morley M, Khanna O, Opara EC, Brey EM.. J Mater Sci Mater Med. 2012;23(4):903-912.

Regenerative medicine as applied to general surgery. Orlando G, Wood KJ, De Coppi P, Baptista PM, Binder KW, Bitar KN, Breuer C, Burnett L, Christ G, Farney A, Figliuzzi M, Holmes JH IV, Koch K, Macchiarini P, Mirmalek Sani S-H, Opara E, Remuzzi A, Rogers J, Saul JM, Seliktar D,.. Ann Surg. 2012;255(5):867-880.

A three-dimensional microfluidic approach to scaling up microencapsulation of cells. Tendulkar S, Mirmalek-Sani SH, Childers C, Saul J, Opara EC, Ramasubramanian MK.. Biomed Microdevices. 2012;14(3):461-469.

Development of ovarian follicle-like structures [abstract]. Joo S, Helms A, Sivanandane S, Jackson JD, Soker S, Lee SJ, Atala A, Opara E, Yoo JJ.. J Tissue Eng Regen Med. 2012;6(Suppl 1):131.

Encapsulated ovarian endocrine cells produced sustained levels of steroid hormones in vivo [abstract]. Sivanandane S, Saul J, Joo S, Pareta R, Arenas J, Marshall N, Yoo J, Atala A, Opara E.. J Tissue Eng Regen Med. 2012;6(Suppl 1):410.

In vitro generation of three-dimensional functional ovarian structures [abstract]. Joo S, Sivanandane S, Opara EC, Jackson JD, Yoo JJ, Atala A.. J Am Coll Surg. 2012;215(3 Suppl):S65.

Immunoisolation: where regenerative medicine meets solid organ transplantation. Pareta R, Sanders B, Babbar P, Soker T, Booth C, McQuilling J, Sivanandane S, Stratta RJ, Orlando G, Opara EC.. Expert Rev Clin Immunol. 2012;8(7):685-692.

Regenerative medicine as applied to solid organ transplantation: current status and future challenges. Orlando G, Baptista P, Birchall M, De Coppi P, Farney A, Opara E, Rogers J, Stratta RJ, Atala A, Soker S, et al.. Transpl Int. 2011;24(3):223-232.

New alginate microcapsule system for angiogenic protein delivery and immunoisolation of islets for transplantation in the rat omentum pouch. McQuilling JP, Arenas-Herrera J, Childers C, Pareta RA, Khanna O, Jiang B, Brey EM, Farney AC, Opara EC.. Transplant Proc. 2011;43(9):3262-3264.

A scalable microfluidic device for the mass production of microencapsulated islets. Tendulkar S, McQuilling JP, Childers C, Pareta R, Opara EC, Ramasubramanian MK.. Transplant Proc. 2011;43(9):3184-3187.

The effect of FGF-1 loaded alginate microbeads on neovascularization and adipogenesis in a vascular pedicle model of adipose tissue engineering. Moya ML, Cheng M-H, Huang J-J, Francis-Sedlak ME, Kao S-w, Opara EC, Brey EM.. Biomaterials. 2010;31(10):2816-2826.

Fibroblast growth factor-1 (FGF-1) loaded microbeads enhance local capillary neovascularization. Moya ML, Garfinkel MR, Liu X, Lucas S, Opara EC, Greisler HP, Brey EM.. J Surg Res. 2010;160(2):208-212.

Design of a bioartificial pancreas. Opara EC, Mirmalek-Sani SH, Khanna O, Moya ML, Brey EM.. J Investig Med. 2010;58(7):831-837.

Synthesis of multilayered alginate microcapsules for the sustained release of fibroblast growth factor-1. Khanna O, Moya ML, Opara EC, Brey EM.. J Biomed Mater Res A. 2010;95(2):632-640.

All Publications

For a listing of recent publications, refer to PubMed, a service provided by the National Library of Medicine.

For a list of earlier publications, visit the Carpenter Library Publication Search.

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