Profile

Fred W. Perrino, Ph.D.Wake Forest School of Medicine

Fred W. Perrino, Ph.D.

Professor,

Contact Information

Academic: 336-716-4349 | Department: 336-716-2011

Email: fperrino@wakehealth.edu

Education & Training

  • B.S., Ohio State University , 1979
  • Ph.D., University of Cincinnati , 1986
  • Fellowship, University of Cincinnati, 1985
  • Fellowship, University of Washington Schoo, 1989

Memberships

  • New York Academy Of Sciences
  • Am Assn Of Cancer Research
  • Am Soc Of Biochem & Mol Biolog
  • Am Soc Of Microbiology
Fred W. Perrino, Ph.D.

Fred W. Perrino, Ph.D.

Professor, Biochemistry
Comprehensive Cancer Center

Research Interests

aids, immunology/allergy/inflammatio, model development, molecular biology/molecular me, rare diseases, structural biology

Contact Information

Academic: 336-716-4349 | Department: 336-716-2011

Email: fperrino@wakehealth.edu

Recent Publications

Fye JM, Coffin SR, Orebaugh CD, Hollis T, Perrino FW. The Arg-62 residues of the TREX1 exonuclease act across the dimer interface contributing to catalysis in the opposing protomers. J Biol Chem. 2014;289(16):11556-65.

Rice GI, Reijns MA, Coffin SR, Forte GM, Anderson BH, Szynkiewicz M, Gornall H, Gent D, Leitch A, Botella MP, Fazzi E, Gener B, Lagae L, Olivieri I, Orcesi S, Swoboda KJ, Perrino FW, Jackson AP, Crow YJ. Synonymous mutations in RNASEH2A create cryptic splice sites impairing RNase H2 enzyme function in Aicardi-Goutieres syndrome. Hum Mutat. 2013;34(8):1066-1070.

Orebaugh CD, Fye JM, Harvey S, Hollis T, Wilkinson JC, Perrino FW. The TREX1 C-terminal region controls cellular localization through ubiquitination. J Biol Chem. 2013;288(40):28881-92.


Pence MG, Blans P, Zink CN, Fishbein JC, Perrino FW. Bypass of N2-ethylguanine by human DNA polymerase kappa. DNA Repair. 2011;10(1):56-64.

Namjou B, Kothari PH, Kelly JA, Glenn SB, Ojwang JO, Adler A, Alarcon-Riquelme ME, Perrino FW, Freedman BI, Langefeld CD, et al. Evaluation of the TREX1 gene in a large multi-ancestral lupus cohort. Genes Immun. 2011;12(4):270-279.

Coffin SR, Hollis T, Perrino FW. Functional consequences of the RNase H2A subunit mutations that cause Aicardi-Goutieres syndrome. J Biol Chem. 2011;286(19):16984-91.

Fye JM, Orebaugh CD, Coffin SR, Hollis T, Perrino FW. Dominant mutations of the TREX1 exonuclease gene in lupus and Aicardi-Goutieres syndrome. J Biol Chem. 2011;286(37):32373-82.


Powell RD, Holland PJ, Hollis T, Perrino FW. Aicardi-Goutieres syndrome gene and HIV-1 restriction factor SAMHD1 is a dGTP-regulated deoxynucleotide triphosphohydrolase. J Biol Chem. 2011;286(51):43596-600.


Pence MG, Blans P, Zink CN, Hollis T, Fishbein JC, Perrino FW. Lesion bypass of N2-ethylguanine by human DNA polymerase Iota. J Biol Chem. 2009;284(3):1732-1740.

Perrino FW, Harvey S, Shaban NM, Hollis T. RNaseH2 mutants that cause Aicardi-Goutieres syndrome are active nucleases. J Mol Med. 2009;87(1):25-30.

Casteel DE, Zhuang S, Zeng Y, Perrino FW, Boss GR, Goulian M, Pilz RB. A DNA polymerase-alpha.primase cofactor with homology to replication protein A-32 regulates DNA replication in mammalian cells. J Biol Chem. 2009;284(9):5807-5818.

de Silva U, Perrino FW, Hollis T. DNA binding induces active site conformational change in the human TREX2 3'-exonuclease. Nucleic Acids Res. 2009;37(7):2411-2417.

Lee-Kirsch M, Gong M, Chowdhury D, Senenko L, Engel K, De Silva U, Bailey SL, Harvey S, Hollis T, Perrino FW, et al. Mutations in the 3 '-5 ' DNA exonuclease TREX1 cause monogenic and complex forms of lupus erythematosus [abstract]. Eur J Pediatr. 2008;167(3):365.

Perrino FW, de Silva U, Harvey S, Pryor EE Jr, Cole DW, Hollis T. Cooperative DNA binding and communication across the dimer interface in the TREX2 3 '--> 5 '-exonuclease. J Biol Chem. 2008;283(31):21441-52.

Lehtinen DA, Harvey S, Mulcahy MJ, Hollis T, Perrino FW. The TREX1 double-stranded DNA degradation activity is defective in dominant mutations associated with autoimmune disease. J Biol Chem. 2008;283(46):31649-56.

de Silva U, Choudhury S, Bailey SL, Harvey S, Perrino FW, Hollis T. The crystal structure of TREX1 explains the 3' nucleotide specificity and reveals a polyproline II helix for protein partnering. J Biol Chem. 2007;282(14):10537-43.

Harrigan JA, Fan J, Momand J, Perrino FW, Bohr VA, Wilson DM III. WRN exonuclease activity is blocked by DNA termini harboring 3 degree obstructive groups. Mech Ageing Dev. 2007;128(3):259-266.

Rice G, Newman WG, Dean J, Patrick T, Parmar R, Flintoff K, Robins P, Harvey S, Hollis T, Perrino FW, et al. Heterozygous mutations in TREX1 cause familial chilblain lupus and dominant Aicardi-Goutieres syndrome. Am J Hum Genet. 2007;80(4):811-815.

Lee-Kirsch MA, Chowdhury D, Harvey S, Gong M, Senenko L, Engel K, Pfeiffer C, Hollis T, Gahr M, Perrino FW, et al. A mutation in TREX1 that impairs susceptibility to granzyme A-mediated cell death underlies familial chilblain lupus. J Mol Med. 2007;85(5):531-537.

Lee-Kirsch MA, Gong M, Chowdhury D, Seneko L, Engel K, Lee Y-A, de Silva U, Bailey SL, Harvey S, Perrino FW, et al. Mutations in the gene encoding the 3 '-5 ' DNA exonuclease TREX1 are associated with systemic lupus erythematosus. Nat Genet. 2007;39(9):1065-1067.

Upton DC, Wang X, Blans P, Perrino FW, Fishbein JC, Akman SA. Mutagenesis by exocyclic alkylamino purine adducts in Escherichia coli. Mutat Res. 2006;599(1-2):1-10.

Upton DC, Wang X, Blans P, Perrino FW, Fishbein JC, Akman SA. Replication of N(2)-ethyldeoxyguanosine DNA adducts in the human embryonic kidney cell line 293. Chem Res Toxicol. 2006;19(7):960-967.

Chowdhury D, Beresford PJ, Zhu P, Zhang D, Sund J-S, Demple B, Perrino FW, Lieberman J. The exonuclease TREX1 is in the SET complex and acts in concert with NM23-H1 to degrade DNA during granzyme A-mediated cell death. Mol Cell. 2006;23(1):133-142.

Kirby TW, Harvey S, DeRose EF, Chalov S, Chikova AK, Perrino FW, Schaaper RM, London RE, Pedersen LC. Structure of the Escherichia coli DNA polymerase III epsilon-HOT proofreading complex. J Biol Chem. 2006;281(50):38466-71.

All Publications

For a listing of recent publications, refer to PubMed, a service provided by the National Library of Medicine.

For a list of earlier publications, visit the Carpenter Library Publication Search.

Professor, Biochemistry

Fred W. Perrino, Ph.D.

Fred W. Perrino, Ph.D.

Professor, Biochemistry
Comprehensive Cancer Center

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