Leslie B. Poole, Ph.D.Wake Forest School of Medicine

Leslie B. Poole, Ph.D.


Clinical Interests

Cancer Cell Biology

Contact Information

Academic: 336-716-6711 | Department: 336-716-6711


Education & Training

  • B.A., Wake Forest University, 1980
  • Ph.D., Wake Forest University, 1988
  • Fellowship, University of Maryland-College, 1991


  • Am Chemical Society
  • Am Soc Of Biochem & Mol Biolog
  • Am Soc of Microbiology
  • Protein Society
  • Society Free Radical Biology
Leslie B. Poole, Ph.D.

Leslie B. Poole, Ph.D.

Professor, Biochemistry
Comprehensive Cancer Center

Research Interests

biotechnology, cancer/oncogenesis, cell growth, differentiation,d, infectious diseases, molecular biology/molecular me, structural biology

Contact Information

Academic: 336-716-6711 | Department: 336-716-6711


Recent Publications

Poole LB. The basics of thiols and cysteines in redox biology and chemistry. Free Radic Biol Med. 2015;80():148-157.

Parsonage D, Nelson KJ, Ferrer-Sueta G, Alley S, Karplus PA, Furdui CM, Poole LB. Dissecting peroxiredoxin catalysis: separating binding, peroxidation, and resolution for a bacterial AhpC. Biochemistry. 2015;54(7):1567-1575.

Poole LB, Schoneich C. Introduction: what we do and do not know regarding redox processes of thiols in signaling pathways. Free Radic Biol Med. 2015;80():145-147.

Collins JA, Poole L, Furdui C, Nelson K, Loeser R. Mitochondrial reactive oxygen species (ROS) promote hyperoxidation of peroxiredoxins and inhibit pro-survival IGF-1 signaling in human articular chondrocytes [abstract]. Osteoarthritis Cartilage. 2015;23(Suppl 2):A32-A33.

Perkins A, Nelson KJ, Parsonage D, Poole LB, Karplus PA. Peroxiredoxins: guardians against oxidative stress and modulators of peroxide signaling. Trends Biochem Sci. 2015;40(8):435-445.

Wood ST, Long D, Reisz J, Yammani R, Burke E, Klomsiri C, Poole L, Furdui C, Loeser R. Cysteine-mediated redox regulation of cell signaling in chondrocytes stimulated with fibronectin fragments [abstract]. Osteoarthritis Cartilage. 2015;23(Suppl 2):A65-A66.

Langston PK, Yang M, Bierbach U, Parsonage D, Poole LB, Price MJ, Grayson JM. Au-ACRAMTU-PEt3 alters redox balance to inhibit T cell proliferation and function. J Immunol. 2015;195(5):1984-1994.

Wood ST, Long DL, Reisz JA, Yammani RR, Burke EA, Klomsiri C, Poole LB, Furdui CM, Loeser RF. Cysteine-mediated redox regulation of cell signaling in chondrocytes stimulated with fibronectin fragments. Arthritis Rheumatol. 2015;():.

Buchko GW, Perkins A, Parsonage D, Poole LB, Karplus PA. Backbone chemical shift assignments for Xanthomonas campestris peroxiredoxin Q in the reduced and oxidized states: a dramatic change in backbone dynamics. Biomol NMR Assign. 2015;():.

Furdui CM, Poole LB. Chemical approaches to detect and analyze protein sulfenic acids. Mass Spectrom Rev. 2014;33(2):126-146.

Klomsiri C, Rogers LC, Soito L, McCauley AK, King SB, Nelson KJ, Poole LB, Daniel LW. Endosomal H2O2 production leads to localized cysteine sulfenic acid formation on proteins during lysophosphatidic acid-mediated cell signaling. Free Radic Biol Med. 2014;71():49-60.

Poole TH, Reisz JA, Zhao W, Poole LB, Furdui CM, King SB. Strained cycloalkynes as new protein sulfenic acid traps. J Am Chem Soc. 2014;136(17):6167-6170.

Randall LM, Manta B, Hugo M, Gil M, Batthyany C, Trujillo M, Poole LB, Denicola A. Nitration transforms a sensitive peroxiredoxin 2 into a more active and robust peroxidase. J Biol Chem. 2014;289(22):15536-43.

Nelson KJ, Parsonage D, Karplus PA, Poole LB. Evaluating peroxiredoxin sensitivity toward inactivation by peroxide substrates. Methods Enzymol. 2013;527():21-40.

Poole LB, Nelson KJ, Karplus PA. Sulfenic acids and peroxiredoxins in oxidant defense In: Jakob U, Reichmann D, eds. Oxidative stress and redox regulation. Dordrecht: Springer;2013: 85-118.

Keyes JD, Nelson K, Parsonage D, Daniel L, Furdui C, Poole L. Modulation of signaling proteins by reversible cysteine modification [abstract]. FASEB J. 2013;27():993.3.

Taneja NK, Ganguly T, Bakaletz LO, Nelson KJ, Dubey P, Poole LB, Deora R. D-alanine modification of a protease-susceptible outer membrane component by the Bordetella pertussis dra locus promotes resistance to antimicrobial peptides and polymorphonuclear leukocyte-mediated killing. J Bacteriol. 2013;195(22):5102-5111.

Perkins A, Nelson KJ, Williams JR, Parsonage D, Poole LB, Karplus PA. The sensitive balance between the fully folded and locally unfolded conformations of a model peroxiredoxin. Biochemistry. 2013;52(48):8708-8721.

Reisz JA, Bechtold E, King SB, Poole LB, Furdui CM. Thiol-blocking electrophiles interfere with labeling and detection of protein sulfenic acids. FEBS J. 2013;280(23):6150-6161.

Poole LB, King SB, inventors; Wake Forest University Health Sciences, assignee. Sulfenic acid-reactive compounds and their methods of synthesis. United States patent US 8,486,642. 2013 July 16. 2013;():.

Karplus PA, Poole LB. Peroxiredoxins as molecular triage agents, sacrificing themselves to enhance cell survival during a peroxide attack [comment]. Mol Cell. 2012;45(3):275-278.

Qian J, Wani R, Klomsiri C, Poole LB, Tsang AW, Furdui CM. A simple and effective strategy for labeling cysteine sulfenic acid in proteins by utilization of beta-ketoesters as cleavable probes. Chem Commun (Camb). 2012;48(34):4091-4093.

Salsbury FR Jr, Yuan Y, Knaggs MH, Poole LB, Fetrow JS. Structural and electrostatic asymmetry at the active site in typical and atypical peroxiredoxin dimers. J Phys Chem B. 2012;116(23):6832-6843.

Cho S-H, Parsonage D, Thurston C, Dutton RJ, Poole LB, Collet J-F, Beckwith J. A new family of membrane electron transporters and its substrates, including a new cell envelope peroxiredoxin, reveal a broadened reductive capacity of the oxidative bacterial cell envelope. MBio. 2012;3(2):e00291-11.

Debnath A, Parsonage D, Andrade RM, He C, Cobo ER, Hirata K, Chen S, Garcia-Rivera G, Orozco E, Martinez MB, Gunatilleke SS, Barrios AM, Arkin MR, Poole LB, McKerrow JH, Reed SL. A high-throughput drug screen for Entamoeba histolytica identifies a new lead and target. Nat Med. 2012;18(6):956-960.

Gretes MC, Poole LB, Karplus PA. Peroxiredoxins in parasites. Antioxid Redox Signal. 2012;17(4):608-633.

Salsbury FR Jr, Poole LB, Fetrow JS. Electrostatics of cysteine residues in proteins: parameterization and validation of a simple model. Proteins. 2012;80(11):2583-2591.

Crump KE, Juneau DG, Poole LB, Haas KM, Grayson JM. The reversible formation of cysteine sulfenic acid promotes B-cell activation and proliferation. Eur J Immunol. 2012;42(8):2152-2164.

Perkins A, Gretes MC, Nelson KJ, Poole LB, Karplus PA. Mapping the active site helix-to-strand conversion of CxxxxC peroxiredoxin Q enzymes. Biochemistry. 2012;51(38):7638-7650.

Lang BS, Gorren ACF, Oberdorfer G, Wenzl MV, Furdui CM, Poole LB, Mayer B, Gruber K. Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2: reaction intermediates revealed by crystallography and mass spectrometry. J Biol Chem. 2012;287(45):38124-34.

Daniel LW, Klomsiri C, Rogers LC, Nelson KJ, Soito L, King SB, Poole LB. Localized hydrogen peroxide-dependent cysteine oxidation is required for lysophosphatidic acid signaling in ovarian and prostate cancer cells [abstract]. Free Radic Biol Med. 2012;53(Suppl 2):S33.

Michalek RD, Crump KE, Weant AE, Hiltbold EM, Juneau DG, Moon EY, Yu DY, Poole LB, Grayson JM. Peroxiredoxin II regulates effector and secondary memory CD8+ T cell responses. J Virol. 2012;86(24):13629-41.

Nelson KJ, Parsonage D, Van Swearingen AED, Yuan Y, Salsbury FR, Hall A, Karplus PA, Poole LB. Specific residues in peroxiredoxins promote peroxide reactivity through effects on cysteine pKa, transition state stabilization and oligomerization [abstract]. Free Radic Biol Med. 2012;53(Suppl 2):S151.

Poole L, Karplus PA, Nelson K, Parsonage D. Active site and interface communication regulating peroxiredoxin functions [abstract]. Free Radic Biol Med. 2012;53(Suppl 2):S8.

Soito L, Williamson C, Knutson ST, Fetrow JS, Poole LB, Nelson KJ. PREX: PeroxiRedoxin classification indEX, a database of subfamily assignments across the diverse peroxiredoxin family. Nucleic Acids Res. 2011;39(Database issue):D332-7.

Nelson KJ, Knutson ST, Soito L, Klomsiri C, Poole LB, Fetrow JS. Analysis of the peroxiredoxin family: using active-site structure and sequence information for global classification and residue analysis. Proteins. 2011;79(3):947-964.

Klomsiri C, Karplus PA, Poole LB. Cysteine-based redox switches in enzymes. Antioxid Redox Signal. 2011;14(6):1065-1077.

Wani R, Qian J, Yin L, Bechtold E, King SB, Poole LB, Paek E, Tsang AW, Furdui CM. Isoform-specific regulation of Akt by PDGF-induced reactive oxygen species. Proc Natl Acad Sci U S A. 2011;108(26):10550-5.

Nirudodhi S, Parsonage D, Karplus PA, Poole LB, Maier CS. Conformational studies of the robust 2-Cys peroxiredoxin Salmonella typhimurium AhpC by solution phase hydrogen/deuterium (H/D) exchange monitored by electrospray ionization mass spectrometry. Int J Mass Spectrom. 2011;302(1-3):93-100.

Hall A, Nelson K, Poole LB, Karplus PA. Structure-based insights into the catalytic power and conformational dexterity of peroxiredoxins. Antioxid Redox Signal. 2011;15(3):795-815.

Qian J, Klomsiri C, Wright MW, King SB, Tsang AW, Poole LB, Furdui CM. Simple synthesis of 1,3-cyclopentanedione derived probes for labeling sulfenic acid proteins. Chem Commun. 2011;47(32):9203-9205.

Poole LB, Hall, A, Nelson KJ. Overview of peroxiredoxins in oxidant defense and redox regulation In: Costa LG, Davila JC, Lawrence DA, Reed DJ, Hodgson E, Coruzzi G, et al Current protocols in toxicology, suppl 49. New York: John Wiley & Sons;2011: 7.9.1-15.

Kaplan N, Urao N, Furuta E, Kim SJ, Razvi M, Nakamura Y, Mckinney RD, Poole LB, Fukai T, Ushio-Fukai M. Localized cysteine sulfenic acid formation by vascular endothelial growth factor: role in endothelial cell migration and angiogenesis. Free Radic Res. 2011;45(10):1124-1135.

Nelson KJ, Rogers LC, Klomsiri C, Soito L, Poole LB, Daniel LW. Localized hydrogen peroxide-dependent cysteine oxidation is required for lysophosphatidic acid signaling in ovarian and prostate cancer cells [abstract]. Free Radic Biol Med. 2011;51(Suppl 1):S137.

Qian J, Klomsiri C, King SB, Poole LB, Tsang AW, Furdui CM. Simple synthesis of chemical probes for labeling sulfenic acid proteins [abstract]. Free Radic Biol Med. 2011;51(Suppl 1):S20.

Clarke TE, Romanov V, Chirgadze YN, Klomsiri C, Kisselman G, Wu-Brown J, Poole LB, Pai EF, Chirgadze NY. Crystal structure of alkyl hydroperoxidase D like protein PA0269 from Pseudomonas aeruginosa: homology of the AhpD-like structural family. BMC Struct Biol. 2011;11():27.

Reeves SA, Parsonage D, Nelson KJ, Poole LB. Kinetic and thermodynamic features reveal that Escherichia coli BCP is an unusually versatile peroxiredoxin. Biochemistry. 2011;50(41):8970-8981.

Bechtold E, Reisz JA, Klomsiri C, Tsang AW, Wright MW, Poole LB, Furdui CM, King SB. Water-soluble triarylphosphines as biomarkers for protein S-nitrosation. ACS Chem Biol. 2010;5(4):405-414.

Parsonage D, Desrosiers DC, Hazlett KRO, Sun Y, Nelson KJ, Cox DL, Radolf JD, Poole LB. Broad specificity AhpC-like peroxiredoxin and its thioredoxin reductant in the sparse antioxidant defense system of Treponema pallidum. Proc Natl Acad Sci U S A. 2010;107(14):6240-6245.

Oshikawa J, Urao N, Kim HW, Kaplan N, Razvi M, McKinney R, Poole LB, Fukai T, Ushio-Fukai M. Extracellular SOD-derived H2O2 promotes VEGF signaling in caveolae/lipid rafts and post-ischemic angiogenesis in mice. PLoS ONE. 2010;5(4):e10189.

All Publications

For a listing of recent publications, refer to PubMed, a service provided by the National Library of Medicine.

For a list of earlier publications, visit the Carpenter Library Publication Search.

Professor, Biochemistry

Clinical Interests

Cancer Cell Biology
Leslie B. Poole, Ph.D.

Leslie B. Poole, Ph.D.

Professor, Biochemistry
Comprehensive Cancer Center

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