Profile

Leslie B. Poole, Ph.D.Wake Forest School of Medicine

Leslie B. Poole, Ph.D.

Professor,


Clinical Specialties

Cancer Cell Biology

Contact Information

Academic: 336-716-6711 | Department: 336-716-6711

Email: lbpoole@wakehealth.edu

Education & Training

  • B.A., Wake Forest University , 1980
  • Ph.D., Wake Forest University , 1988
  • Fellowship, University of Maryland-College, 1991

Memberships

  • Protein Society
  • Society Free Radical Biology
  • Am Chemical Society
  • Am Soc Of Biochem & Mol Biolog
  • Am Soc Of Microbiology
Leslie B. Poole, Ph.D.

Leslie B. Poole, Ph.D.

Professor, Biochemistry
Comprehensive Cancer Center
Translational Science Institute

Research Interests

biotechnology, cancer/oncogenesis, cell growth, differentiation,d, immunology/allergy/inflammatio, infectious diseases, structural biology

Contact Information

Academic: 336-716-6711 | Department: 336-716-6711

Email: lbpoole@wakehealth.edu

Recent Publications

Furdui CM, Poole LB. Chemical approaches to detect and analyze protein sulfenic acids. Mass Spectrom Rev. 2014;33(2):126-146.

Klomsiri C, Rogers LC, Soito L, McCauley AK, King SB, Nelson KJ, Poole LB, Daniel LW. Endosomal H2O2 production leads to localized cysteine sulfenic acid formation on proteins during lysophosphatidic acid-mediated cell signaling. Free Radic Biol Med. 2014;71():49-60.

Poole TH, Reisz JA, Zhao W, Poole LB, Furdui CM, King SB. Strained cycloalkynes as new protein sulfenic acid traps. J Am Chem Soc. 2014;136(17):6167-6170.

Randall LM, Manta B, Hugo M, Gil M, Batthyany C, Trujillo M, Poole LB, Denicola A. Nitration transforms a sensitive peroxiredoxin 2 into a more active and robust peroxidase. J Biol Chem. 2014;289(22):15536-43.

Nelson KJ, Parsonage D, Karplus PA, Poole LB. Evaluating peroxiredoxin sensitivity toward inactivation by peroxide substrates. Methods Enzymol. 2013;527():21-40.

Poole LB, Nelson KJ, Karplus PA. Sulfenic acids and peroxiredoxins in oxidant defense In: Jakob U, Reichmann D, eds. Oxidative stress and redox regulation. Dordrecht: Springer;2013: 85-118.

Keyes JD, Nelson K, Parsonage D, Daniel L, Furdui C, Poole L. Modulation of signaling proteins by reversible cysteine modification [abstract]. FASEB J. 2013;27():993.3.


Perkins A, Nelson KJ, Williams JR, Parsonage D, Poole LB, Karplus PA. The sensitive balance between the fully folded and locally unfolded conformations of a model peroxiredoxin. Biochemistry. 2013;52(48):8708-8721.

Reisz JA, Bechtold E, King SB, Poole LB, Furdui CM. Thiol-blocking electrophiles interfere with labeling and detection of protein sulfenic acids. FEBS J. 2013;280(23):6150-6161.

Poole LB, King SB, inventors; Wake Forest University Health Sciences, assignee. Sulfenic acid-reactive compounds and their methods of synthesis. United States patent US 8,486,642. 2013 July 16. 2013;():.


Qian J, Wani R, Klomsiri C, Poole LB, Tsang AW, Furdui CM. A simple and effective strategy for labeling cysteine sulfenic acid in proteins by utilization of beta-ketoesters as cleavable probes. Chem Commun (Camb). 2012;48(34):4091-4093.

Salsbury FR Jr, Yuan Y, Knaggs MH, Poole LB, Fetrow JS. Structural and electrostatic asymmetry at the active site in typical and atypical peroxiredoxin dimers. J Phys Chem B. 2012;116(23):6832-6843.


Debnath A, Parsonage D, Andrade RM, He C, Cobo ER, Hirata K, Chen S, Garcia-Rivera G, Orozco E, Martinez MB, Gunatilleke SS, Barrios AM, Arkin MR, Poole LB, McKerrow JH, Reed SL. A high-throughput drug screen for Entamoeba histolytica identifies a new lead and target. Nat Med. 2012;18(6):956-960.

Gretes MC, Poole LB, Karplus PA. Peroxiredoxins in parasites. Antioxid Redox Signal. 2012;17(4):608-633.

Salsbury FR Jr, Poole LB, Fetrow JS. Electrostatics of cysteine residues in proteins: parameterization and validation of a simple model. Proteins. 2012;80(11):2583-2591.

Crump KE, Juneau DG, Poole LB, Haas KM, Grayson JM. The reversible formation of cysteine sulfenic acid promotes B-cell activation and proliferation. Eur J Immunol. 2012;42(8):2152-2164.

Perkins A, Gretes MC, Nelson KJ, Poole LB, Karplus PA. Mapping the active site helix-to-strand conversion of CxxxxC peroxiredoxin Q enzymes. Biochemistry. 2012;51(38):7638-7650.

Lang BS, Gorren ACF, Oberdorfer G, Wenzl MV, Furdui CM, Poole LB, Mayer B, Gruber K. Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2: reaction intermediates revealed by crystallography and mass spectrometry. J Biol Chem. 2012;287(45):38124-34.

Daniel LW, Klomsiri C, Rogers LC, Nelson KJ, Soito L, King SB, Poole LB. Localized hydrogen peroxide-dependent cysteine oxidation is required for lysophosphatidic acid signaling in ovarian and prostate cancer cells [abstract]. Free Radic Biol Med. 2012;53(Suppl 2):S33.

Michalek RD, Crump KE, Weant AE, Hiltbold EM, Juneau DG, Moon EY, Yu DY, Poole LB, Grayson JM. Peroxiredoxin II regulates effector and secondary memory CD8+ T cell responses. J Virol. 2012;86(24):13629-41.

Nelson KJ, Parsonage D, Van Swearingen AED, Yuan Y, Salsbury FR, Hall A, Karplus PA, Poole LB. Specific residues in peroxiredoxins promote peroxide reactivity through effects on cysteine pKa, transition state stabilization and oligomerization [abstract]. Free Radic Biol Med. 2012;53(Suppl 2):S151.

Poole L, Karplus PA, Nelson K, Parsonage D. Active site and interface communication regulating peroxiredoxin functions [abstract]. Free Radic Biol Med. 2012;53(Suppl 2):S8.

Soito L, Williamson C, Knutson ST, Fetrow JS, Poole LB, Nelson KJ. PREX: PeroxiRedoxin classification indEX, a database of subfamily assignments across the diverse peroxiredoxin family. Nucleic Acids Res. 2011;39(Database issue):D332-7.

Nelson KJ, Knutson ST, Soito L, Klomsiri C, Poole LB, Fetrow JS. Analysis of the peroxiredoxin family: using active-site structure and sequence information for global classification and residue analysis. Proteins. 2011;79(3):947-964.

Klomsiri C, Karplus PA, Poole LB. Cysteine-based redox switches in enzymes. Antioxid Redox Signal. 2011;14(6):1065-1077.

Wani R, Qian J, Yin L, Bechtold E, King SB, Poole LB, Paek E, Tsang AW, Furdui CM. Isoform-specific regulation of Akt by PDGF-induced reactive oxygen species. Proc Natl Acad Sci U S A. 2011;108(26):10550-5.


Hall A, Nelson K, Poole LB, Karplus PA. Structure-based insights into the catalytic power and conformational dexterity of peroxiredoxins. Antioxid Redox Signal. 2011;15(3):795-815.

Qian J, Klomsiri C, Wright MW, King SB, Tsang AW, Poole LB, Furdui CM. Simple synthesis of 1,3-cyclopentanedione derived probes for labeling sulfenic acid proteins. Chem Commun. 2011;47(32):9203-9205.


Kaplan N, Urao N, Furuta E, Kim SJ, Razvi M, Nakamura Y, Mckinney RD, Poole LB, Fukai T, Ushio-Fukai M. Localized cysteine sulfenic acid formation by vascular endothelial growth factor: role in endothelial cell migration and angiogenesis. Free Radic Res. 2011;45(10):1124-1135.

Nelson KJ, Rogers LC, Klomsiri C, Soito L, Poole LB, Daniel LW. Localized hydrogen peroxide-dependent cysteine oxidation is required for lysophosphatidic acid signaling in ovarian and prostate cancer cells [abstract]. Free Radic Biol Med. 2011;51(Suppl 1):S137.

Qian J, Klomsiri C, King SB, Poole LB, Tsang AW, Furdui CM. Simple synthesis of chemical probes for labeling sulfenic acid proteins [abstract]. Free Radic Biol Med. 2011;51(Suppl 1):S20.

Clarke TE, Romanov V, Chirgadze YN, Klomsiri C, Kisselman G, Wu-Brown J, Poole LB, Pai EF, Chirgadze NY. Crystal structure of alkyl hydroperoxidase D like protein PA0269 from Pseudomonas aeruginosa: homology of the AhpD-like structural family. BMC Struct Biol. 2011;11():27.

Reeves SA, Parsonage D, Nelson KJ, Poole LB. Kinetic and thermodynamic features reveal that Escherichia coli BCP is an unusually versatile peroxiredoxin. Biochemistry. 2011;50(41):8970-8981.

Bechtold E, Reisz JA, Klomsiri C, Tsang AW, Wright MW, Poole LB, Furdui CM, King SB. Water-soluble triarylphosphines as biomarkers for protein S-nitrosation. ACS Chem Biol. 2010;5(4):405-414.

Parsonage D, Desrosiers DC, Hazlett KRO, Sun Y, Nelson KJ, Cox DL, Radolf JD, Poole LB. Broad specificity AhpC-like peroxiredoxin and its thioredoxin reductant in the sparse antioxidant defense system of Treponema pallidum. Proc Natl Acad Sci U S A. 2010;107(14):6240-6245.

Oshikawa J, Urao N, Kim HW, Kaplan N, Razvi M, McKinney R, Poole LB, Fukai T, Ushio-Fukai M. Extracellular SOD-derived H2O2 promotes VEGF signaling in caveolae/lipid rafts and post-ischemic angiogenesis in mice. PLoS ONE. 2010;5(4):e10189.

Nelson KJ, Klomsiri C, Codreanu SG, Soito L, Liebler DC, Rogers LC, Daniel LW, Poole LB. Use of dimedone-based chemical probes for sulfenic acid detection methods to visualize and identify labeled proteins. Methods Enzymol. 2010;473():95-115.

Klomsiri C, Nelson KJ, Bechtold E, Soito L, Johnson LC, Lowther WT, Ryu S-E, King SB, Furdui CM, Poole LB. Use of dimedone-based chemical probes for sulfenic acid detection evaluation of conditions affecting probe incorporation into redox-sensitive proteins. Methods Enzymol. 2010;473():77-94.

Parsonage D, Reeves SA, Karplus PA, Poole LB. Engineering of fluorescent reporters into redox domains to monitor electron transfers. Methods Enzymol. 2010;474():1-21.

Yuan Y, Knaggs MH, Poole LB, Fetrow JS, Salsbury FR Jr. Conformational and oligomeric effects on the cysteine pKa of tryparedoxin peroxidase. J Biomol Struct Dyn. 2010;28(1):51-70.

Hall A, Parsonage D, Poole LB, Karplus PA. Structural evidence that peroxiredoxin catalytic power is based on transition-state stabilization. J Mol Biol. 2010;402(1):194-209.

Flaherty NL, Mellilo AA, Rashel M, Poole LB, Melendez JA. Defining the antioxidant determinants of Francisella tularensis virulence [abstract]. Free Radic Biol Med. 2010;49(Suppl 1):S142.

Saunders JA, Rogers LC, Klomsiri C, Poole LB, Daniel LW. Reactive oxygen species mediate lysophosphatidic acid induced signaling in ovarian cancer cells. Free Radic Biol Med. 2010;49(12):2058-2067.

King SB, Poole L, inventors; Wake Forest University Health Sciences, assignee. Sulfenic acid-reactive compounds. United States patent US 7,803,630. 2010 Sept 28. 2010;():.

Hall A, Karplus PA, Poole LB. Typical 2-Cys peroxiredoxins--structures, mechanisms and functions. FEBS J. 2009;276(9):2469-2477.

Hall A, Parsonage D, Horita D, Karplus PA, Poole LB, Barbar E. Redox-dependent dynamics of a dual thioredoxin fold protein: evolution of specialized folds. Biochemistry. 2009;48(25):5984-5993.

Hall A, Sankaran B, Poole LB, Karplus PA. Structural changes common to catalysis in the Tpx peroxiredoxin subfamily. J Mol Biol. 2009;393(4):867-881.

Klomsiri C, Poole LB, Nelson KJ, Rogers L, Klorig EB, Daniel LW, King SB, Furdui CM. Detection and identification of oxidative cysteine modifications in proteins involved in signal transduction pathways [abstract]. Free Radic Biol Med. 2009;47(Suppl 1):S17-S18.

Klorig EB, Reisz JA, Klomsiri C, Wright MW, Day CS, Poole LB, Furdui CM, King B. The reaction of S-nitrosothiols with a water-soluble phosphine gives S-alkyl phosphonium products as potential bio-markers [abstract]. Free Radic Biol Med. 2009;47(Suppl 1):S32.

Kaplan N, Urao N, Razvi M, Nakamura Y, Poole LB, Fukai T, Ushio-Fukai M. ROS-dependent cysteine sulfenic acid formation of IQGAP1 and ERK1/2 in VEGF-induced endothelial cell proliferation and migration [abstract]. Circulation. 2009;120(18 Suppl 2):S1085.

Yamamoto Y, Ritz D, Planson A-G, Jonsson TJ, Faulkner MJ, Boyd D, Beckwith J, Poole LB. Mutant AhpC peroxiredoxins suppress thiol-disulfide redox deficiencies and acquire deglutathionylating activity. Mol Cell. 2008;29(1):36-45.

Poole LB, Nelson KJ. Discovering mechanisms of signaling-mediated cysteine oxidation. Curr Opin Chem Biol. 2008;12(1):18-24.

Salsbury FR Jr, Knutson ST, Poole LB, Fetrow JS. Functional site profiling and electrostatic analysis of cysteines modifiable to cysteine sulfenic acid. Protein Sci. 2008;17(2):299-312.


Parsonage D, Karplus PA, Poole LB. Substrate specificity and redox potential of AhpC, a bacterial peroxiredoxin. Proc Natl Acad Sci U S A. 2008;105(24):8209-8214.

Smith-Pearson PS, Kooshki M, Spitz DR, Poole LB, Zhao W, Robbins ME. Decreasing peroxiredoxin II expression decreases glutathione, alters cell cycle distribution, and sensitizes glioma cells to ionizing radiation and H2O2. Free Radic Biol Med. 2008;45(8):1178-1189.

Nelson KJ, Parsonage D, Hall A, Karplus A, Poole LB. Cysteine pKa values for the bacterial peroxiredoxin AhpC. Biochemistry. 2008;47(48):12860-68.

Hutson SM, Poole LB, Coles S, Conway ME. Redox regulation and trapping sulfenic acid in the peroxide-sensitive human mitochondrial branched chain aminotransferase. Methods Mol Biol. 2008;476():139-152.

Nakamura Y, Kaplan N, Poole LB, Fukai T, Ushio-Fukai M. Detection of cysteine sulfenic acid formation: role in reactive oxygen species-dependent VEGF signaling linked to endothelial migration [abstract]. Circulation. 2008;118(18 Suppl 2):S273.


Allen EE, Diao L, Fetrow JS, John DJ, Loeser RF, Poole LB.The shuffle index and evaluation of models of signal transduction pathways


Poole LB, Klomsiri C, Knaggs SA, Furdui CM, Nelson KJ, Thomas MJ, Fetrow JS, Daniel LW, King SB. Fluorescent and affinity-based tools to detect cysteine sulfenic acid formation in proteins. Bioconjug Chem. 2007;18(6):2004-2017.

Michalek RD, Nelson KJ, Holbrook BC, Yi JS, Stridiron D, Daniel LW, Fetrow JS, King SB, Poole LB, Grayson JM. The requirement of reversible cysteine sulfenic acid formation for T cell activation and function. J Immunol. 2007;179(10):6456-6467.

Poole LB. The catalytic mechanism of peroxiredoxins. Subcell Biochem. 2007;44():61-81.

Klomsiri C, Nelson KJ, Naggs SA, Fetrow JS, King SB, Furdui CM, Poole LB. Cysteine sulfenic acid detection in signal transduction pathways [abstract]. Free Radic Biol Med. 2007;43(Suppl 1):S157.

Nelson KJ, Klomsiri C, Rogers RL, Daniel LW, Poole LB. The role of cysteine sulfenic acid formation during NF-kappa B-mediated signaling [abstract]. Free Radic Biol Med. 2007;43(Suppl 1):S156.

Parsonage D, Nelson KJ, Day AE, Poole LB. Substrate specificity and chemical characterization of a bacterial peroxiredoxin, AhpC [abstract]. Free Radic Biol Med. 2007;43(Suppl 1):S113.

King SB, Poole L, Fetrow J, inventors; Wake Forest University Health Sciences, assignee. Sulfenic acid-reactive compounds and their methods of synthesis. United States patent US 7,294,748. 2007 Nov 13. 2007;():.

Phalen TJ, Weirather K, Deming PB, Anathy V, Howe AK, van der Vliet A, Jonsson TJ, Poole LB, Heintz NH. Oxidation state governs structural transitions in peroxiredoxin II that correlate with cell cycle arrest and recovery. J Cell Biol. 2006;175(5):779-789.

Loeser RF, Yammani R, Erickson E, King B, Poole L. Chondrocyte MMP-13 expression in response to alpha 5 beta 1 integrin stimulation requires protein sulfenic acids as signaling intermediates [abstract]. Arthritis Rheum. 2006;54(9 Suppl):S318.

All Publications

For a listing of recent publications, refer to PubMed, a service provided by the National Library of Medicine.

For a list of earlier publications, visit the Carpenter Library Publication Search.

Professor, Biochemistry

Clinical Specialties

Cancer Cell Biology

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