Metabolomics is the science of identifying small-molecule metabolites in organisms, tissues, cells, and biofluids. Metabolomics provides a snapshot of a sample’s metabolite content and can identify up-/down-regulation of pathways and processes as a function of genotype, physiology, pathology, and environment. As with other “omics” strategies such as genomics and proteomics, metabolomics seeks to quantitate all detectable components, allowing tracking of ~20-1000 metabolites per experiment. This allows the investigator both to identify and propose new models and to test multivariate models.
The Chemical Biology Core Facility offers NMR- and LCMS-based metabolite profiling services. These methods are able to identify and quantitate numerous small-molecule metabolites present in biofluids, tissue extracts, and cell extracts. Typical sample requirements range from 10 to 200 mg for NMR analysis and 1 to 50 microg for LCMS analysis. For both NMR and MS analyses, metabolites must be extracted into aqueous phase. In general, this is done via methanol:water:chloroform extraction of biofluids or homogenized tissues/cells. Specific protocols are sample dependent, please contact Core Personnel for details.
Costs vary by number of samples, sample concentration, and level of involvement by Core Personnel, but range from $10-75 per sample. Pilot data can be acquired on a time-available basis at reduced cost.
Contact: Ralph S. Orkiszewski