Cellular Imaging Shared Resource

Cellular Imaging

The Cellular Imaging Shared Resource is a full-service microscopy laboratory supplying equipment and technical support for electron, fluorescence and confocal microscopy; it is available throughout the Comprehensive Cancer Center of Wake Forest University and the entire institution for both clinical and research purposes. The Cellular Imaging Shared Resource is equipped to carry out all phases of sample preparation for electron microscopy with a staff that includes a technical engineer/manager and two technologists.

This resource exists in a biomedical research environment and supports numerous investigators whose interests encompass both basic and clinical medical science. The Cancer Center provides additional support for microscopy services to investigators who are members.

Although the orientation is biomedical, the facility supports a wide range of research. Faculty and staff also have extensive experience in non-biomedical zoology, botany, and materials science imaging.

Director

The Director of the Cellular Imaging Shared Resource is Ryan Mott, MD, a research pathologist with clinical specialities in neuropathology and surgical pathology. Dr. Mott also has several years of experience in basic science research, including animal models.

Services We Provide 

The EM equipment includes conventional transmission and scanning electron microscopy (TEM and SEM). The facility also houses a:

  • Laser scanning confocal microscope (LSCM)
  • Digital upright and inverted fluorescence microscopes
  • Time-lapse microscopy
  • Laser capture microdissection
  • Single cell microinjection

Additionally, the laboratory offers numerous specialized research techniques, user training, consultation and collaboration.

Capabilities

  • TEM (120 keV)
  • LSCM with multiphoton excitation
  • Bright field, DIC, phase contrast, polarizing, fluorescence 
  • Immunolabeling for LM, EM, SEM and LSCM
  • Enzyme cytochemistry
  • Cyromicrotomy and EDX compositional microanalysis
  • Image processing and analysis
  • Laser capture microdissection
  • Single cell microinjection

 Instrumentation  

  • FEI Technai BioTwin 120 keV TEM with digital imaging  
  • Zeiss LSM510 laser scanning confocal system
  • Arcturus PixCell II laser microdissection system
  • Zeiss Axioplan 2 and Olympus IX-70 fluorescence microscopes
  • Zeiss Axiovert time-lapse video microscopy system for cultured cells
  • Reichert Ultracut microtomes and cryoultramicrotomes
  • Computer workstations with image processing, analysis, and 3-D reconstruction software
  • Complete specimen preparation facilities

Quick Reference

Cellular Imaging Core

Phone 336-716-2670

Director
Ryan Mott

Phone 336-716-2630

rmott@wakehealth.edu

Supervisor and Chief EM Technologist
Ken Grant

Phone 336-716-6175

kgrant@wakehealth.edu

EM Technologist
Paula Graham

Phone 336-716-2670

pmoore@wakehealth.edu

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Last Updated: 12-16-2014
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