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PCR Lab

Polymerase Chain Reaction (PCR)-based technology allows detection of DNA or RNA sequences that may be specific for certain microorganisms, genes, or gene products. Currently the PCR lab offers the following testing:

  • BCR-ABL quantitative, p120 major breakpoint 
  • BCR-ABL quantitative, p190 minor breakpoint 
  • BRAF mutational analysis
  • EGFR mutational analysis
  • Human papilloma virus (HPV), 14 high risk types including HPV types 16 and 18
  • Immunoglobulin heavy chain gene rearrangement
  • KRAS mutational analysis
  • PML-RARa, quantitative, bcr 1 (long) and bcr 3 (short) forms
  • T-cell receptor gamma gene rearrangement 

All specimens submitted for analysis must be accompanied by a requistion form.  

    Samples for PCR may be submitted in several ways:

    • Paraffin embedded tissue, on glass slides
    • Paraffin block   
    • Blood and bone marrow should be collected in EDTA only. Samples for PCR testing from body fluids require a dedicated sample. Tubes that have been opened and samples for other tests will be rejected (at the director's discretion).

    Normal turnaround time is estimated to be 3-5 days depending upon the day the sample is received.

    Please contact Dr. Stacey O"Neill for any PCR related questions.

     

    Quick Reference

    PCR Lab

    Lab Number: 336-716-9880

    Interim Medical Director, Molecular Diagnostics
    Michael B. Cohen, MD

    Tel: 336-716-5080
    Fax: 336-716-7595

    mbcohen@wakehealth.edu

    Acting Director of Molecular Pathology
    Stacey O'Neill, MD PhD

    Tel: 336-713-2969

    soneill@wakehealth.edu

    Manager
    Martha Ward

    Tel: 336-716-2109
    Fax: 336-716-5890

    mward@wakehealth.edu

    PCR Specialist
    Nora Zbieranski

    Tel: 336-716-9880

    nzbinski@wakehealth.edu

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    Last Updated: 08-10-2017
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    Disclaimer: The information on this website is for general informational purposes only and SHOULD NOT be relied upon as a substitute for sound professional medical advice, evaluation or care from your physician or other qualified health care provider.

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