Mark O. Lively

Dr. Lively directs the Protein Analysis Core Laboratory (PACL) of the Comprehensive Cancer Center of Wake Forest University (CCCWFU). In addition to his responsibilities to the core laboratory, he also conducts research. He is an expert protein and peptide chemist who collaborates with a wide range of research projects that require expertise in protein analysis. He is also an investigator on the NIH funded Community Clinical Oncology Program (CCOP) Research Base team.

Shared Resource Laboratory     

The CCCWFU Protein Analysis Core Lab was established by Dr. Lively in 1983. Services provided by the PACL currently include: protein identification by mass spectrometry; bioinformatics analysis of DNA, proteins, and peptides; amino acid sequence analysis of proteins and peptides; quantitative amino acid composition analyses; high performance liquid chromatography of proteins, peptides, amino acids, and other biological molecules. Services that are now available commercially and no longer offered by the PACL include: chemical synthesis of peptides (1988-2008); oligonucleotide synthesis (1990-2011); Sanger sequencing of DNA (1996-2012). The PACL consistently received “outstanding” rankings from NCI site visit teams over the last 30 years of competitive renewal applications for the CCCWFU. See http://www.wakehealth.edu/Bioanalytical-Laboratory/Protein-Analysis-Lab.htm

Research Projects  

  1. Correlation of Oropharyngeal Pepsin and Gastroesophageal Reflux
    This project is a clinical study in a pediatric population to validate the use of a custom ELISA assay for gastric pepsin measured in saliva as a diagnostic indicator of gastroesophageal reflux disease (GERD) in pediatric patients. Pepsin concentrations in saliva samples are obtained from patients undergoing testing by multichannel intraluminal impedance (MII) with pH measurement. A correlation between oropharyngeal pepsin and pH/MII will greatly enhance the ability to diagnose GERD in a non-invasive way to guide appropriate use of PPIs. Dr. Lively developed the ELISA method for quantification of concentrations of gastric pepsin, an enzyme that is produced only in the stomach. The presence of pepsin in the oropharynx indicates that patients have experience recent reflux events that have crossed the upper esophageal junction.
      
  2. Comprehensive Cancer Center of Wake Forest University CCOP Research Base. 
    Dr. Lively provides logistics support, sample processing and tracking, and HPLC analytical services to the CCOP Research Base protocols. A recently completed project was a randomized double-blind placebo-controlled study of oral coenzyme Q10 to relieve self-reported cancer treatment related fatigue in breast cancer patients. Dr. Lively’s lab analysed all plasma samples for CoQ-10 and vitamin E. A similar study was a phase II randomized placebo controlled, double blinded trial to evaluate the effects of fruit and vegetable extracts on intermediate biomarkers in head and neck cancer patients. Dr. Lively’s lab analyzed a range of different lipid-soluble antioxidants in plasma samples. The lab is currently providing logistical support to a study
    to develop and validate prediction biomarkers for radiation therapy-induced acute and chronic skin reactions and quality of life in five racial/ethnic groups of breast cancer patients: Whites, Black/African Americans; Hispanic/Latinos; Asians/Native Hawaiians/Pacific Islanders; and American Indians/Alaskan Natives. This project will recruit 1000 breast cancer patients.
  3. Ephrin A1 released from cancer cells.
    In a collaborative study with the laboratory of Waldemar Debenski, Dr. Lively was able to show that ephrinA1, a GPI-linked ligand for the EphA2 receptor, which is overexpressed in glioblastoma (GBM) is released from the cell surface by metalloprotease cleavage. Using mass spectrometry, three different forms of ephrinA1 were discovered in cell media of cells overexpressing the protein.

 

Quick Reference

Biochemistry

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Last Updated: 12-11-2014
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