Waters Q-TOF ApcI US
LockSpray enabled exact mass measurements
The mass accuracy variation is less than 3 ppm
Lower detection limit is less than 3 fmole
Data Directed Analysis
Digital Dead Time Correction Technology
Interfaced with a Waters Cap LC | The Q-TOF gives investigators access to new, powerful proteomic technology while at the same time facilitating analysis of complex lipids. Q-TOF advantages include precise mass measurement (± 3 ppm), sensitivity (< 3 fmole) coupled with the MS/MS capability of sequencing peptides and analyze post-translational modifications. Protein identification is conveniently performed on proteolytic digests (generally trypsin) that are separated on a capillary HPLC column before analysis. Samples are usually separated by 1-dimensional PAGE before digestion. The Q-TOF was purchased with funds from the NIH Shared Instrumentation Program. The Q-TOF was recently interfaced to an Advion Nanomate source that increased sensitivity and reduce ion suppression. Funding was obtained from the North Carolina Biotechnology Center. |