Mass Spectrometer

Quadrupole Time-of-Flight Mass Spectrometer

QTOF

Features

Waters Q-TOF ApcI US

LockSpray enabled exact mass measurements
The mass accuracy variation is less than 3 ppm

Lower detection limit is less than 3 fmole

Data Directed Analysis

Digital Dead Time Correction Technology

Interfaced with a Waters Cap LC

The Q-TOF gives investigators access to new, powerful proteomic technology while at the same time facilitating analysis of complex lipids. Q-TOF advantages include precise mass measurement (± 3 ppm), sensitivity (< 3 fmole) coupled with the MS/MS capability of sequencing peptides and analyze post-translational modifications. Protein identification is conveniently performed on proteolytic digests (generally trypsin) that are separated on a capillary HPLC column before analysis. Samples are usually separated by 1-dimensional PAGE before digestion. The Q-TOF was purchased with funds from the NIH Shared Instrumentation Program. The Q-TOF was recently interfaced to an Advion Nanomate source that increased sensitivity and reduce ion suppression. Funding was obtained from the North Carolina Biotechnology Center.

 

Quick Reference

Mass Spectrometer Facility
Department of Biochemistry
Wake Forest School of Medicine
391 Technology Way
Building A1, Room 340
Winston-Salem, NC 27101
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Last Updated: 04-14-2014
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