Thermo Scientific TSQ Discovery Max and a Waters Quattro II
Off-axis electrospray sources
Mass accuracy about 0.01%
Lower detection limit approximately
True precursor and neutral loss analysis
The TSQ is interfaced to an Agilent model 1100 LC with autosampler. The Quattro II is interfaced to a Symbosis LC/sample processing system
These tandem mass spectrometers give investigators access to a powerful technology for the analysis of complex mixtures of lipids, intact proteins, peptides and other metabolites. They have the advantage of precise mass measurement at the lower mass range of many lipids coupled with the capability for precursor and neutral loss analysis to identify glycerophospholipid subclasses. Product ion analysis is used to identify the fatty acids carried by the glycerophospholipid. The Quattro II was purchased with grant funds from the NSF Academic Research Infrastructure Program. It was updated with funds from the North Carolina Biotechnology Center and the Winston-Salem Foundation. The TSQ was purchased with funds from the North Carolina Biotechnology Center.