Analytical Imaging Facility

Services We Provide

INSTRUMENTATION

The instrumentation available includes a Typhoon Trio and an Amersham Imager-600 RGB multi-label imager.

  • Typhoon Trio
    The Typhoon Imager allows for indirect phosphor imaging of 3H, 14C, 125I, 32P, 33P, 35S or other radioactive signals from gels or membranes. Users provide their own phosphor storage screens for these applications; Image Erasers are available in the facility.  The Typhoon Trio has three excitation lasers (488/532/633) for direct excitation of red-, green-, and blue-excited fluorophores as well as chemifluorescent samples. With six emission filters, this allows for scanning of up to four fluorescent dyes in one sample
  • Amersham Imager-600 RGB
    This instrument is a multi-label imager capable of capturing and analyzing images from DNA gels, chemiluminescent and fluorescent Western blots (red, blue and green fluorophores), and Coomassie or silver-stained protein gels.

SOFTWARE

ImageQuant 5.2 and ImageQuantTL 8.1 software is available on computer in the facility for the analysis of 8 to 16 bit grayscale TIFF, .gel, or .ds files captured by these or other imagers.

 USAGE AND TRAINING

  • Users pay an annual user fee, which allows for unlimited scanning on any of the instruments.
  • Usage of the instruments must be recorded on the provided sign-in sheets.
    • New users on any of the instruments must first attend a training session.

    For training on use of the imagers, please contact Dr. Linda Metheny-Barlow (336-713-7636). 

    Quick Reference

    Analytical Imaging Facility
    Dr. Linda Metheny-Barlow

    336-716-7636

    lmetheny@wakehealth.edu

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    Disclaimer: The information on this website is for general informational purposes only and SHOULD NOT be relied upon as a substitute for sound professional medical advice, evaluation or care from your physician or other qualified health care provider.

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