Analytical Imaging Facility
Services We Provide
The instrumentation available includes a Typhoon
Trio and an Amersham Imager-600 RGB multi-label imager.
- Typhoon Trio
The Typhoon Imager allows for indirect phosphor imaging of 3H,
14C, 125I, 32P, 33P, 35S
or other radioactive signals from gels or membranes. Users provide their own
phosphor storage screens for these applications; Image Erasers are available in
the facility. The Typhoon Trio has three excitation lasers (488/532/633)
for direct excitation of red-, green-, and blue-excited fluorophores as well as
chemifluorescent samples. With six emission filters, this allows for scanning
of up to four fluorescent dyes in one sample
- Amersham Imager-600 RGB
This instrument is a
multi-label imager capable of capturing and analyzing images from DNA gels,
chemiluminescent and fluorescent Western blots (red, blue and green
fluorophores), and Coomassie or silver-stained protein gels.
ImageQuant 5.2 and ImageQuantTL
8.1 software is available on computer in the facility for the analysis of 8 to 16 bit
grayscale TIFF, .gel, or .ds files captured by these or other imagers.
USAGE AND TRAINING
- Users pay an annual user fee,
which allows for unlimited scanning on any of the instruments.
- Usage of the instruments must be
recorded on the provided sign-in sheets.
New users on any of the instruments must first
attend a training session.
For training on use of the imagers, please contact Dr. Linda Metheny-Barlow