Biomolecular Resource Facility
The Biomolecular Resource Facility (BRF) provides advanced technologies for protein and DNA analysis. The laboratory is supported by a combination of support from the Comprehensive Cancer Center of Wake Forest University, and user fees. The laboratory is directed by Prof. Mark Lively and has two full time research laboratory technicians. Computer support is provided by Mr. Chris Williamson, Operations Technical Support Analysis for the Department of Biochemistry.
The major techniques employed in the BRF include:
- High performance liquid chromatography methods and equipment for preparative purification of proteins; analytical
characterization of peptides, metabolites, and amino acids
- Peptide and protein identification and characterization by mass spectrometry and in-gel digestion of proteins; Amino acid
sequence analysis of proteins and peptides by Edman degradation
- DNA/RNA synthesis - Custom synthesis and labels.
- ELISA methods – Implementation of commercial ELISA methods: Pepsin; CRP
- Clinical Specimen Logistics – collection, shipping, receiving, processing, and ultra-low storage of samples for clinical studies.
Mark O. Lively, Professor of Biochemistry
Wake Forest Biotech Place
2015 Research Core Symposium Presentation