Cell and Viral Vector Core Laboratory (CVVCL)

On this page, you will find:

• About Us
• Our Equipment
• Director
• Published Research
• Contact Us

 About Us

The mission of the Cell and Viral Vector Core Laboratory (CVVCL) is to support and enhance the research efforts of cancer researchers at the Comprehensive Cancer Center of Wake Forest University.

The Shared Resource serves two essential and highly utilized functions within the Cancer Center:

• Laboratory Support for Experimental Approaches
  Specialized cell culture and experimental services are available through the experienced and highly skilled CVVCL staff, and allow investigators to carry out short-term experiments quickly and in a cost-efficient manner. These experiments include establishment of cell lines from primary murine and human tissue explants, production of virus stocks for gene transfer, and large-scale expansion of tumor cell lines for downstream applications.

• Distribution Center for Cell Culture Products
  The CVVCL distribution center provides cell culture products. Both basic and specialty media are available to investigators at a substantial discount compared to the list price or general university discount. Molecular biology reagents are available from five different vendors, and reflect the breadth of products sold by these vendors. Cancer Center members receive substantial discounts on these products, and all users benefit from free shipping.

Please place a product order through our online ordering system.

Our Equipment

Specialized equipment such as the Caliper IVIS100 In Vivo Imaging System, maintained by the CVVCL, increases the versatility of experimental techniques accessible to CCC members. We provide training sessions for new users of the machine, and the CVVCL is responsible for machine upkeep. 

Director

Purnima Dubey, PhD

Published Research

• Barnes AP, Miller BE, Kucera GL. Cyclooxygenase inhibition and hyperthermia for the potentiation of the cytotoxic response in ovarian cancer cells. Gynecol Oncol 2007;104(2):443-50.
• Hu Y, Sun H, Owens RT, et al. Decorin suppresses prostate tumor growth through inhibition of epidermal growth factor and androgen receptor pathways. Neoplasia 2009; 11(10):1042-53.
• Knovich MA, Lawson HL, Burke MH, McCoy TP, Owen J. Rapid quantitative assay of ADAMTS13 activity on an automated coagulation analyzer: clinical applications and comparison with immunoblot method. Am J Hematol 2008;83(8):654-6.
• Mallakin A, Sugiyama T, Taneja P, et al. Mutually exclusive inactivation of DMP1 and ARF/p53 in lung cancer. Cancer Cell 2007;12(4):381-94. 
• Nichols GJ, Schaack J, Ornelles DA. Widespread phosphorylation of histone H2AX by species C adenovirus infection requires viral DNA replication. J Virol 2009;83(12):5987-98.
• Seeds MC, Peachman KK, Bowton DL, Sivertson KL, Chilton FH. Regulation of arachidonate remodeling enzymes impacts eosinophil survival during allergic asthma. Am J Respir Cell Mol Biol 2009;41(3):358-66. 
• Taneja P, Mallakin A, Matise LA, Frazier DP, Choudhary M, Inoue K. Repression of Dmp1 and Arf transcription by anthracyclins: critical roles of the NF-kappaB subunit p65. Oncogene 2007; 26(53): 7457-66.
• El Gazzar M, McCall CE. MicroRNAs distinguish translational from transcriptional silencing during endotoxin tolerance. J Biol Chem 2010; 285:20940-51.
• Markert CD, Meaney MP, Voelker KA, Grange RW, Dalley HW, Cann JK, Ahmed M, Bishwokarma B, Walker SJ, Yu SX, Brown M, Lawlor MW, Beggs AH, Childers MK. Functional muscle analysis of the Tcap knockout mouse. Hum Mol Genet 2010;19:2268-83.
• Roberts, A. L., Connolly, K. L., Doern, C. D., Holder, R. C., Reid, S. D. Loss of the group A Streptococcus regulator Srv decreases biofilm formation in vivo in an otitis media model of infection. Infect Immun, 2010. In Press.
• Cao, P., Deng, Z., Wan, M., Huang W., Cramer S.D., Xu, J., Lei, M., Sui, G. MicroRNA-101 negatively regulates Ezh2 and its expression is modulated by androgen receptor and HIF-1a/HIF-1b. Molecular Cancer, 2010;9:108-19.
• Lewis, E.M., Sergeant, S., Ledford, B., Stull, N., Dinauer, M.C., McPhail, L.C. Phosphorylation of p22phox on threonine 147 enhances NADPH oxidase activity by promoting p47phox binding. J Biol Chem 2010;285:2959-67.

Contact Us

Dr. Purnima Dubey, Director
pdubey@wakehealth.edu

Cell and Viral Vector Core Laboratory
Mr. Nathan Iyer and Ms. Terry Bennett
cvvcl@wakehealth.edu