Flow Cytometry Shared Resource

Areas of Focus

The typical experiments handled by the Flow Cytometry Shared Resource Facility are designed to do analysis or to sort based on up to 9 fluorescent parameters. Some representative examples of analysis experiments include the:

  • Measurement of DNA content for cell cycle analysis
  • Cell cycle specific nuclear and cytoplasmic proteins
  • Apoptosis markers
  • Substituted deoxyuridine incorporation
  • Stem cell side populations
  • Reactive oxygen species
  • Cell surface markers using labeled antibodies or substrates
  • Cell viability
  • Intracellular ion concentration or pH
  • Membrane potential

Cell lysates can also be analyzed with bead substrate based immunoassays. Some representative examples of sorting experiments include:

  • Purification of cells transfected with fluorescent protein tagged DNA inserts
  • Isolation of cell populations expressing specific intracellular or membrane metabolic products. 

Quick Reference

Flow Cytometry Shared Resource

Lab 336-716-9465

James Wood



Martha Alexander-Miller



Beth Holbrook



Find A Doctor Ways to Give
Last Updated: 05-11-2016
Five Wake Forest Baptist Specialties Earn U.S. News RankingsComprehensive Cancer Centers National Designation is RenewedMagnet Hospital Recognition2015 Best DoctorsJoint Commission Report

Disclaimer: The information on this website is for general informational purposes only and SHOULD NOT be relied upon as a substitute for sound professional medical advice, evaluation or care from your physician or other qualified health care provider.

© Wake Forest Baptist Medical Center, Medical Center Boulevard, Winston-Salem, NC 27157. All Rights Reserved.