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Flow Cytometry Shared Resource

Areas of Focus

The typical experiments handled by the Flow Cytometry Shared Resource Facility are designed to do analysis or to sort based on up to 9 fluorescent parameters. Some representative examples of analysis experiments include the:

  • Measurement of DNA content for cell cycle analysis
  • Cell cycle specific nuclear and cytoplasmic proteins
  • Apoptosis markers
  • Substituted deoxyuridine incorporation
  • Stem cell side populations
  • Reactive oxygen species
  • Cell surface markers using labeled antibodies or substrates
  • Cell viability
  • Intracellular ion concentration or pH
  • Membrane potential

Cell lysates can also be analyzed with bead substrate based immunoassays. Some representative examples of sorting experiments include:

  • Purification of cells transfected with fluorescent protein tagged DNA inserts
  • Isolation of cell populations expressing specific intracellular or membrane metabolic products. 

Quick Reference

Flow Cytometry Shared Resource

Lab 336-716-9465

James Wood, PhD

336-716-9465
336-716-4461

jawood@wakehealth.edu

Jason M. Grayson, MD

336-716-0268

jgrayson@wakehealth.edu

Beth Holbrook

336-716-5938

bcholbro@wakehealth.edu

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Last Updated: 05-11-2016
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