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Severe Weather Alert - Updated: 11:30 am, January 17, 2018

Due to rapidly changing weather conditions, all Wake Forest Baptist Health clinics will be closing today (January 17, 2018) at 2 pm, and will reopen tomorrow (January 18, 2018) by 10 am. 
Urgent Care – Clemmons and Urgent Care – Mocksville will be closing today (January 17) at 4 pm, and will reopen tomorrow (January 18) by 10 am.
If you have questions, please contact the clinic directly.


Flow Cytometry Shared Resource

Areas of Focus

The typical experiments handled by the Flow Cytometry Shared Resource Facility are designed to do analysis or to sort based on up to 9 fluorescent parameters. Some representative examples of analysis experiments include the:

  • Measurement of DNA content for cell cycle analysis
  • Cell cycle specific nuclear and cytoplasmic proteins
  • Apoptosis markers
  • Substituted deoxyuridine incorporation
  • Stem cell side populations
  • Reactive oxygen species
  • Cell surface markers using labeled antibodies or substrates
  • Cell viability
  • Intracellular ion concentration or pH
  • Membrane potential

Cell lysates can also be analyzed with bead substrate based immunoassays. Some representative examples of sorting experiments include:

  • Purification of cells transfected with fluorescent protein tagged DNA inserts
  • Isolation of cell populations expressing specific intracellular or membrane metabolic products. 

Quick Reference

Flow Cytometry Shared Resource

Lab 336-716-9465

James Wood, PhD

336-716-9465
336-716-4461

jawood@wakehealth.edu

Jason M. Grayson, MD

336-716-0268

jgrayson@wakehealth.edu

Beth Holbrook

336-716-5938

bcholbro@wakehealth.edu

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