Flow Cytometry Shared Resource
Services We Provide
Use of the Instruments
The institutional instruments available as part of the Flow
Cytometry Shared Resource include:
Located at the Biotech Place
- BD FACS
Aria cell sorter (3 lasers and 11 parameters)
tips available: 70 micron, 100 micron & 130 micron.
of single cell sorting into 24 and 96 well plates
is fitted with a special flow cell to provide better precision for measuring
DNA content and side-populations
- BD FACS
Canto II analyzer (3 lasers and 10 parameters)*
- BD FACS
Calibur analyzers (2 lasers and 6 parameters)*
Located at Hanes Building, 4th floor, Room 4063
Accuri C6 analyzer (2 lasers and 6 parameters)*
addition to the software available with each flow cytometer, FCS Express flow
cytometer analysis software is available to analyze the multiparameter data to
identify and characterize cell subpopulations. DNA histogram analysis software
programs (ModFit and MultiCycle) are also available to address the needs of
researchers requiring advanced cell cycle analysis.
* Instruments are available to use 24 hours, 7 days a week.
Users must seek the appropriate instruction to be approved instrument
operators. See below.
to Use the Equipment
The Flow Cytometry Shared Resource uses iLab's Core Facility Management Software as an online system to streamline the process of ordering and billing for service requests. Please access iLab to make a reservation.
instrument utilization is as follows:
- FACS Calibur: $55/hour
- BD Accuri C6: $60/hour
- FACS Canto: $60/hour
- FACS Aria: cell sorting $85/hour, plus a $40 set-up fee
Cancer Center members receive a
30 percent subsidy for cancer-related projects.
The Director provides consultative services at no charge to the
investigator for discussions regarding experiment design, execution or
interpretation. In many cases, this saves the investigator time and resources
by preventing mistakes in the initial experimental design that may not be
obvious to less experienced users. Investigators also have access to the wide
network of pre-eminent flow cytometry users from academia and industry via
personal introductions by Dr. Wood, who has extensive connections in this
Hands-on training is
provided for individuals who would like the ability to acquire their data on
the FACS Calibur, BD Accuri C6 or FACS Canto. Dr. Martha Alexander-Miller
should be contacted directly to schedule for the one of the monthly training
sessions for the FACS Calibur. The training for the BD Accuri is coordinated by
Dr. James Wood. The training for the FACS Canto is coordinated by Dr. Martha