Flow Cytometry Shared Resource

Services We Provide

Use of the Instruments

The institutional instruments available as part of the Flow Cytometry Shared Resource include:

Located at the Biotech Place

  • BD FACS Aria cell sorter (3 lasers and 11 parameters)
    • Sorting tips available:  70 micron, 100 micron & 130 micron.
    • Capable of single cell sorting into 24 and 96 well plates
    • Instrument is fitted with a special flow cell to provide better precision for measuring DNA content and side-populations
  • BD FACS Canto II analyzer (3 lasers and 10 parameters)*
  • BD FACS Calibur analyzers (2 lasers and 6 parameters)*

Located at Hanes Building, 4th floor, Room 4063

  • BD Accuri C6 analyzer (2 lasers and 6 parameters)*
  • In addition to the software available with each flow cytometer, FCS Express flow cytometer analysis software is available to analyze the multiparameter data to identify and characterize cell subpopulations. DNA histogram analysis software programs (ModFit and MultiCycle) are also available to address the needs of researchers requiring advanced cell cycle analysis.

* Instruments are available to use 24 hours, 7 days a week. Users must seek the appropriate instruction to be approved instrument operators. See below.

Reservations to Use the Equipment 

The Flow Cytometry Shared Resource uses iLab's Core Facility Management Software as an online system to streamline the process of ordering and billing for service requests. Please access iLab to make a reservation.

Cost for instrument utilization is as follows: 

  • FACS Calibur:  $55/hour
  • BD Accuri C6:  $60/hour
  • FACS Canto:  $60/hour
  • FACS Aria: cell sorting $85/hour, plus a $40 set-up fee

Cancer Center members receive a 30 percent subsidy for cancer-related projects.​

Consultative Services

The Director provides consultative services at no charge to the investigator for discussions regarding experiment design, execution or interpretation. In many cases, this saves the investigator time and resources by preventing mistakes in the initial experimental design that may not be obvious to less experienced users. Investigators also have access to the wide network of pre-eminent flow cytometry users from academia and industry via personal introductions by Dr. Wood, who has extensive connections in this field.

Training

Hands-on training is provided for individuals who would like the ability to acquire their data on the FACS Calibur, BD Accuri C6 or FACS Canto. Dr. Martha Alexander-Miller should be contacted directly to schedule for the one of the monthly training sessions for the FACS Calibur. The training for the BD Accuri is coordinated by Dr. James Wood. The training for the FACS Canto is coordinated by Dr. Martha Alexander-Miller. 

 

 

 

Quick Reference

Flow Cytometry Shared Resource

Lab 336-716-9465

James Wood

336-716-9465
336-716-4461

jawood@wakehealth.edu

Martha Alexander-Miller

336-716-5936

marthaam@wakehealth.edu

Beth Holbrook

336-716-5938

bcholbro@wakehealth.edu

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Last Updated: 08-15-2016
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