| Metabolomics Service | Full Internal Rate* | External Rate |
Targeted Metabolomics Analysis (1 column)
Quantification of metabolites using
a single chromatographic column. Minimum sample required for analysis
is 5-10 mg for tissues, and 50-100 ul for biofluids.
Metabolite List | $50.78 | $79.22 |
Targeted Metabolomics Analysis (2 columns)
Quantification of metabolites using
two chromatographic columns. Minimum sample required for analysis is 5-10
mg for tissues, and 50-100 ul for biofluids.
Metabolite List | $101.57 | $158.45 |
| Pharmacokinetic Studies | $57.23 | $89.28 |
Metabolomics Method Development
Price
for novel method development on a per hour basis.
| $100.24 | $156.37 |
| Metabolite and Other Small Molecule Molecular Weight Determination | $35.00 | $54.60 |
| Free Cholesterol (Less than 10 samples) | $61.57 | $96.05 |
| Free Cholesterol (10 or more samples) | $44.23 | $69.00 |
| Total Cholesterol (Less than 10 samples) | $61.57 | $96.05 |
| Total Cholesterol (10 or more samples) | $44.23 | $69.00 |
Cholesteryl Esters
The individual cholesteryl ester species quantified include 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 20:3, 20:4, 20:5, and 22:6. 17:0 cholesteryl ester is used as an internal standard. Data is presented as the mole percent and concentration of each species normalized to total protein, tissue weight, or plasma volume.
| $40.00 | $62.40 |
Oxysterols (Less than 10 samples)
The individual species quantified include 7α-hydroxycholesterol, 7β-hydroxycholesterol, 24- hydroxycholesterol, 25- hydroxycholesterol, 27- hydroxycholesterol, 7-ketocholesterol, 5α,6α-epoxycholestanol, 5β,6β-epoxycholestanol, 7α-OH-25-OH-cholesterol, 7β-OH-25-OH-cholesterol, and 7α-OH-27-OH-cholesterol. d7-24-hydroxycholesterol, d3-25-hydroxycholesterol, d7-7-ketocholesterol, and d5-27-OH-cholesterol are used as internal standards. | $83.46 | $130.20 |
Oxysterols (10 or more samples)
The individual species quantified include 7α-hydroxycholesterol, 7β-hydroxycholesterol, 24- hydroxycholesterol, 25- hydroxycholesterol, 27- hydroxycholesterol, 7-ketocholesterol, 5α,6α-epoxycholestanol, 5β,6β-epoxycholestanol, 7α-OH-25-OH-cholesterol, 7β-OH-25-OH-cholesterol, and 7α-OH-27-OH-cholesterol. d7-24-hydroxycholesterol, d3-25-hydroxycholesterol, d7-7-ketocholesterol, and d5-27-OH-cholesterol are used as internal standards.
| $51.23 | $79.92 |
Free Fatty Acids (Less than 10 samples)
The individual fatty acid species
quantified include 14:0, 14:1, 16:0, 16:1, 18:0, 18:1 (oleate and vaccinate),
18:2(ω6), 18:3(ω3), 18:3(ω6), 20:0, 20:1, 20:2(ω6), 20:3(ω3), 20:4(ω6),
20:5(ω3), 22:0, 22:1, 22:5(ω3), 22:6(ω3), 24:0, 24:1, 24:5(ω3), and 24:6(ω3).
15:0 and 21:0 are used as internal standards. Measurement of additional
species, such as 6:0, 8:0, etc., are available upon request only.
| $63.57 | $99.17 |
Free Fatty Acids (10 or more samples)
The individual fatty acid species
quantified include 14:0, 14:1, 16:0, 16:1, 18:0, 18:1 (oleate and vaccinate),
18:2(ω6), 18:3(ω3), 18:3(ω6), 20:0, 20:1, 20:2(ω6), 20:3(ω3), 20:4(ω6),
20:5(ω3), 22:0, 22:1, 22:5(ω3), 22:6(ω3), 24:0, 24:1, 24:5(ω3), and 24:6(ω3).
15:0 and 21:0 are used as internal standards. Measurement of additional species,
such as 6:0, 8:0, etc., are available upon request only. | $44.23 | $69.00 |
Total Fatty Acids (Less than 10 samples)
The individual fatty acid species
quantified include 14:0, 14:1, 16:0, 16:1, 18:0, 18:1 (oleate and vaccinate),
18:2(ω6), 18:3(ω3), 18:3(ω6), 20:0, 20:1, 20:2(ω6), 20:3(ω3), 20:4(ω6), 20:5(ω3),
22:0, 22:1, 22:5(ω3), 22:6(ω3), 24:0, 24:1, 24:5(ω3), and 24:6(ω3). 15:0 and
21:0 are used as internal standards. Measurement of additional species, such as
6:0, 8:0, etc., are available upon request only.
| $61.57 | $96.05 |
Total Fatty Acids (10 or more samples)
The individual fatty acid species
quantified include 14:0, 14:1, 16:0, 16:1, 18:0, 18:1 (oleate and vaccinate),
18:2(ω6), 18:3(ω3), 18:3(ω6), 20:0, 20:1, 20:2(ω6), 20:3(ω3), 20:4(ω6),
20:5(ω3), 22:0, 22:1, 22:5(ω3), 22:6(ω3), 24:0, 24:1, 24:5(ω3), and 24:6(ω3).
15:0 and 21:0 are used as internal standards. Measurement of additional
species, such as 6:0, 8:0, etc., are available upon request only.
| $42.23 | $65.88 |
HETE/Prostaglandin Metabolites
Hydroxyicosatetraenoic
acid (HETE) and Prostaglandin (PG) metabolite analysis by LC-MS – Metabolites
are detected using a Thermo-Scientific TSQ Discovery Max LC-MS. Species
quantified include 5-HETE, 12-HETE, 15-HETE, 20-HETE, 5-HEPE, 12-HHT, 5-oxo-ETE,
LTB4, 9-HODE, 13-HODE, PGE2, and PGD2. The following deuterated species are
used as internal standards: d4-PGD2, d4-PGE2, d4-9-HODE, d4-13-HODE, d4-LTB4,
d7-5-oxo-ETE, d8-5-HETE, d8-12-HETE, d8-15-HETE, and d8-20-HETE. | $70.78 | $110.42 |
Phospholipid Species
Phospholipid class composition
analysis by LC-MS – Phospholipid species (PG, PE, PS, PI, PA, PC, SM, and LPC)
are separated by LC-MS, and each fraction collected. Total phosphorus assay is
performed on each phospholipid fraction. Data is presented as the mole percent
of total phosphorus for each class.
| $104.46 | $162.96 |
Phospholipid Fatty Acid Species
Phospholipid species are separated by LC-MS, and each
fraction collected. The individual fatty acid species are released by
saponification, converted to their methyl esters with methanolic BF3, and then
quantified by GC-MS. Species quantified include 12:0, 14:0, 14:1, 16:0, 16:1,
18:0, 18:1, 18:2, 18:3, 20:0, 20:1, 20:2, 20:3, 20:4, 20:5, 22:0, 22:1, 22:5, 22:6,
24:0, 24:5, and 24:6 phosphatidylcholine (PC), phosphatidylethanolamine (PE),
phosphatidic acid (PA), phosphatidylserine (PS), phosphatidylinositol (PI), and
phosphatidylglycerol (PG). di-15:0 LPC, and di-17:0 PG, PE, PC, PS, and PA are
used as internal standards.
| $146.69 | $228.84 |
Lysophospholipid Species
The individual species quantified include 12:0, 14:0, 16:0,
16:1, 18:0, 18:1, 18:2, 18:3, 20:0, 20:1, 20:2, 20:3, 20:4, 22:4, 22:5, 22:6,
and 24:0 lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE),
lysophosphatidic acid (LPA), lysophosphatidylserine (LPS),
lysophosphatidylinositol (LPI), and lysophosphatidylglycerol (LPG). d31-16:0
LPI, LPG, LPE, LPC, LPS, and LPA are used as internal standards.
| $104.46 | $162.96 |
Triglyceride Species
The individual fatty acid species quantified include 14:0,
14:1, 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 18:3, 20:0, 20:1, 20:2, 20:3, 20:4,
20:5, 22:0, 22:1, 22:5, 22:6, 24:0, 24:1, 24:5, and 24:6.
| $73.35 | $114.43 |
Ceramide Species Analysis
The individual species quantified
include ceramides 14:0, 14:1, 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 20:0, 20:1,
20:2, 20:3, 20:4, 20:5, 22:0, 22:1, 22:2, 22:5, 22:6, 24:0, 24:1, 24:5, and
24:6.
| $57.23 | $89.28 |
| Sphingosine-1-Phosphate | $70.68 | $110.26 |
F2-Isoprostanes
Total isoprostanes analysis by GC-MS. d4-PGF2α is used as the internal
standard. Data is presented as the concentration of PGF2α, and total
isoprostanes, normalized to tissue weight, total protein, or plasma volume.
| $105.58 | $164.70 |
| Proteomics Service | Full Internal Rate* | External Rate |
| Protein Molecular Weight using Thermo Q-Exactive HF MS | $71.20 | $111.07 |
Protein Identification using Thermo Q-Exactive HF MS
Protein
in-gel digestion, qualitative MS/MS analysis, and database search. | $190.70 | $297.49 |
Proteomics Method Development
Method
development for SRM analysis of single target protein. 3 biological samples
(cells or tissues) are required for analytical method setup. Cost will increase
for multiple targets in a PRM analysis. | $1,131.80 | $1,765.61 |
Analysis of Post-translational Modifications (PTMs)
Protein in-gel/in-solution
digestion, modified peptide enrichment, qualitative MS/MS analysis, and data
processing for identification of PTMs. | $371.04 | $578.82 |
Targeted Proteomics with SRM/PRM Analysis
Protein in-gel/in-solution
digestion, quantitative MS/MS analysis, and data processing for relative
quantitation. AQUA peptide(s) as internal standard(s) may be utilized if
required, but with additional cost. | $291.10 | $454.12 |
Relative Quantitative Analysis using SILAC
Protein
in-gel/in-solution digestion, MS analysis, and data processing for relative
quantitation. Workflow only applies to cell culture studies. | $1,333.30 | $2,079.95 |
Relative Quantitative Analysis using Tandem Mass Tagging (TMT)
Tandem mass tagging of peptides for
relative quantitation. Includes in-solution protein digestion, TMT labeling,
peptide fractionation (10), qualitative MS/MS analysis, and data processing for
search and relative quantitation. Thermo TMT isobaric labeling reagent is
utilized. | $1,482.00 | $2,311.92 |
Data Processing
Per hour cost for time spent on data
analysis, data interpretation, and/or figure construction. This service
includes database search and data compilation for global protein identification
using Proteome Discoverer and PEAKS. Data processes for label-free quantification,
reporter ion quantification and SILAC quantification are also available. Sieve,
Skyline and Spectronaut are utilized for targeted proteomics, such as single or
parallel reaction monitoring (SRM/PRM) analyses. Ingenuity Pathway Analysis
is also available. | $50.20 | $78.31 |
| MALDI-TOF Protein/Peptide Mass Analysis | $75.00 | $117.00 |
Protein Sequencing (1-5 amino acids)
Protein
sequencing using Edman degradation (first five amino acid residues). | $200.00 | $312.00 |
Protein Sequencing (>5 amino acids)
Protein
sequencing using Edman degradation (each additional amino acid residue after
the 5th). | $30.00 | $46.80 |
HPLC Setup Time
Setup
charge for HPLC analysis (required for all HPLC services). | $100.00 | $78.00 |
Automated HPLC
Automated analytical HPLC analysis.
[Columns: 2.1mm, 3.9mm, 4.6m; Reverse Phase: C4, C8, C18; Size Exclusion (TSK) 2000,3000,4000] Detection:UV/VIS; wavelength 190nm-700nm programmable channels along with
multiple wavelengths at a time. Other detectors available: PDA, Fluorescence,
and Wyatt: SEC-MALS, & QELS | $50.00 | $78.00 |
Manual HPLC from semi-micro to Preparative
Manual analytical HPLC analysis. [Columns: C4&C8 2.1mm; Ion exchange DEAE, MonoQ, MonoS, Poros R1/H; Prep columns: Porasil [40x100]; RP C8 [10 x 100 ] & RP C4 [25x100, 40x100];C18 [8x100, 25x100, 40x100, 40x200mm] Detection via UV-Vis dual wavelength detector. Metallic free system available for purifying biologically active proteins. | $75.00 | $117.00 |
HPLC Gel Filtration
HPLC
gel filtration analysis. Size Exclusion chromatography columns Superdex
200, & Sephacryl
S-300HR. TSK-2000, TSK3000, TSK4000SW | $200.00 | $312.00 |