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Proteomics and Metabolomics Shared Resource

Fee Schedule

Metabolomics Services and Fees
Proteomics Services and Fees 

*10% discount for CCC members
**The setup-fee is a flat fee associated with every set of samples being analyzed by a particular service. This fee encompasses instrument preparation, standard preparation, as well as instrument and labor time.


Metabolomics ServiceFull Internal Rate
(per sample)*
External Rate 
(per sample)
Targeted Metabolomics Analysis
Quantification of metabolites in a single metabolic pathway. Minimum sample required for analysis is 5-10 mg for tissues, and 50-100 ul for biofluids. Analytes are detected using the Shimadzu-8050 UHPLC-MS/MS. Pathways include: TCA cycle, Glycolytic System, Pentose-phosphate pathway, Methylation and transsulfuration cycle, Amino acids, Nucleotides, Nucleosides, Co-enzymes, Organic Acids, and other primary metabolites. Please see the Metabolite List for help deciding what pathways to choose. Service is provided on a per pathway basis.

Note: A Setup-fee is associated with this analysis. For each batch of samples, an internal rate of $325.34 or an external rate of $507.53 will be applied. This fee is only applied at a per-batch rate and is in addition to the sample fee. Each additional pathway analyzed for a single batch will incur an internal fee of $128.92 or external fee of $201.12.
$50.78
**Setup-fee: $325.34
$79.22
**Setup-fee: $507.53
Glutathione and Cysteine Redox Couples Measurement
Quantification of Glutathione and GSSG as well as Cysteine and Cystine by LC-MS. Minimum sample required for analysis is 5-10 mg for tissues, and 50-100 ul for biofluids. Oxidation is blocked using a thiol-blocker and then the biological sample is extracted and submitted to instrumental anlysis to determine redox ratios. 

Note:
A Setup-fee is associated with this analysis. For each batch of samples, an internal rate of $196.42 or an external rate of $306.42 will be applied. This fee is only applied at a per-batch rate and is in addition to the sample fee.
$63.68
**Setup-fee: 
$196.42
$99.33
**Setup-fee: 
$306.42
Pharmacokinetic Studies
Example: Coumarin and diazepam metabolite analysis – Coumarin and diazepam metabolites are detected using a Shimadzu-8050 UHPLC-MS/MS. Species quantified include 7-OH coumarin, 7-OH coumarin glucuronide, 7-ethoxycoumarin, diazepam, nordiazepam, temazepam, and oxazepam. 4-OH coumarin is used as the internal standard. Data is presented as the concentration of each metabolite normalized to tissue weight, total protein, or plasma volume.  
Other drugs are tested at a similar price per sample. If a known analysis method is not already available, then method development costs will be added to the final total.

Note: A Setup-fee is associated with this analysis. For each batch of samples, an internal rate of $203.92 or an external rate of $318.12 will be applied. This fee is only applied at a per-batch rate and is in addition to the sample fee.
$60.98
**Setup-fee: 
$203.92
$95.13
**Setup-fee:
$318.12
Metabolomics Method Development 
Price for novel method development on a per hour basis. Includes an estimated costs for reagents and standards.
$100.24$156.37
Metabolite and Other Small Molecule Molecular Weight Determination
Direct injection of standards and/or compounds of interest for molecular weight determination. Samples must be free of salts and detergents. Additional costs apply for sample clean-up.

$35.00$54.60
Free Cholesterol
Quantification of free cholesterol by LC-MS – Cholesterol is detected using a Thermo-Scientific TSQ Quantum Discovery Max LC-MS. Cholesterol-3,4-13C2 is used as the internal standard. Data is presented as the concentration of free cholesterol, normalized to total protein, tissue weight, or plasma volume. If the total cholesterol measurement was also requested, then the amount of esterified cholesterol will be reported as well. Minimum plasma volume required for analysis is 5 ul. For tissues and cells, minimum sample required for analysis will vary depending on the type of tissue or cells.

Note: A Setup-fee is associated with this analysis. For each batch of samples, an internal rate of $62.23 or an external rate of $97.08 will be applied. This fee is only applied at a per-batch rate and is in addition to the sample fee.
$74.46
**Setup-fee:
$62.23
$116.16

**Setup-fee:
$97.08

Total Cholesterol
Quantification of total cholesterol by LC-MS – Samples are saponified, and cholesterol detected using a Thermo-Scientific TSQ Quantum Discovery Max LC-MS. Cholesterol-3,4-13C2 is used as the internal standard. Data is presented as the concentration of total cholesterol, normalized to total protein, tissue weight, or plasma volume. If the free cholesterol measurement was also requested, then the amount of esterified cholesterol will be reported as well. Minimum plasma volume required for analysis is 5 ul. For tissues and cells, minimum sample required will vary depending on the type of tissue or cells. 

Note: A Setup-fee is associated with this analysis. For each batch of samples, an internal rate of $62.23 or an external rate of $97.08 will be applied. This fee is only applied at a per-batch rate and is in addition to the sample fee.
$87.35
**Setup-fee:
$62.23 
$136.27
**Setup-fee:
$97.08
Cholesteryl Esters 
Cholesteryl ester fatty acid species are detected using a Thermo-Scientific TSQ Discovery Max LC-MS.  The individual fatty acid species quantified include 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 20:3, 20:4, 20:5, and 22:6.  17:0 cholesteryl ester is used as an internal standard.  Data is presented as the mole percent and concentration of each species normalized to total protein, tissue weight, or plasma volume. Minimum plasma volume required for analysis is 5 ul. For tissues and cells, minimum sample required will vary depending on the type of tissue or cells. 

Note: A Setup-fee is associated with this analysis. For each batch of samples, an internal rate of $37.23 or an external rate of $58.08 will be applied. This fee is only applied at a per-batch rate and is in addition to the sample fee.
$61.57
**Setup-fee:
$37.23 
$96.05
**Setup-fee:
$58.08
Oxysterols
Quantification of oxysterols by GC-MS – Oxysterols species are detected using a Thermo-Scientific TSQ Quantum XLS GC-MS. The individual species quantified include 7α-hydroxycholesterol, 7β-hydroxycholesterol, 24- hydroxycholesterol, 25- hydroxycholesterol, 27- hydroxycholesterol, 7-ketocholesterol, 5α,6α-epoxycholestanol, 5β,6β-epoxycholestanol, 7α-OH-25-OH-cholesterol, 7β-OH-25-OH-cholesterol, and 7α-OH-27-OH-cholesterol. d7-24-hydroxycholesterol, d3-25-hydroxycholesterol, d7-7-ketocholesterol, and d5-27-OH-cholesterol are used as internal standards. Data is presented as the concentration of each species, normalized to total protein, tissue weight, or plasma volume. Minimum plasma volume required for analysis is 20 ul. For tissues and cells, minimum sample required will vary depending on the type of tissue or cells. 

Note: A Setup-fee is associated with this analysis. For each batch of samples, an internal rate of $798.52 or an external rate of $1,245.69 will be applied. This fee is only applied at a per-batch rate and is in addition to the sample fee.
$83.46
**Setup-fee:
$798.52
$130.20
**Setup-fee:
$1,245.69
Free Fatty Acids
Quantification of free fatty acid species by GC-MS – Free fatty acids are isolated by solid phase extraction, converted to their methyl esters with methanolic BF3, and detected using a Thermo-Scientific TSQ Quantum XLS GC-MS. The individual fatty acid species quantified include 14:0, 14:1, 16:0, 16:1, 18:0, 18:1 (oleate and vaccinate), 18:2(ω6), 18:3(ω3), 18:3(ω6), 20:0, 20:1, 20:2(ω6), 20:3(ω3), 20:4(ω6), 20:5(ω3), 22:0, 22:1, 22:5(ω3), 22:6(ω3), 24:0, 24:1, 24:5(ω3), and 24:6(ω3). 15:0 and 21:0 are used as internal standards. Measurement of additional species, such as 6:0, 8:0, etc., are available upon request only. Data is presented as the concentration of each species normalized to total protein, tissue weight, or plasma volume, along with the mole percent and mass percent of each species. The ratio of ω3 to ω6 fatty acids is also provided. Minimum plasma volume required for analysis is 50 ul. For tissues and cells, minimum sample required will vary depending on the type of tissue or cells. 

Note: A Setup-fee is associated with this analysis. For each batch of samples, an internal rate of $125.58 or an external rate of $195.90 will be applied. This fee is only applied at a per-batch rate and is in addition to the sample fee.
$76.46
**Setup-fee:
$125.58
$119.28
**Setup-fee:
$195.90
Total Fatty Acids
Quantification of total fatty acid species by GC-MS – Samples are saponified, the released fatty acids converted to their methyl esters with methanolic BF3, and detected using a Thermo-Scientific TSQ Quantum XLS GC-MS. The individual fatty acid species quantified include 14:0, 14:1, 16:0, 16:1, 18:0, 18:1 (oleate and vaccinate), 18:2(ω6), 18:3(ω3), 18:3(ω6), 20:0, 20:1, 20:2(ω6), 20:3(ω3), 20:4(ω6), 20:5(ω3), 22:0, 22:1, 22:5(ω3), 22:6(ω3), 24:0, 24:1, 24:5(ω3), and 24:6(ω3). 15:0 and 21:0 are used as internal standards. Measurement of additional species, such as 6:0, 8:0, etc., are available upon request only. Data is presented as the concentration of each species normalized to total protein, tissue weight, or plasma volume, along with the mole and mass percent of each species. The ratio of ω3 to ω6 fatty acids is also provided. Minimum plasma volume required for analysis is 25 ul. For tissues and cells, minimum sample required will vary depending on the type of tissue or cells. 

Note: A Setup-fee is associated with this analysis. For each batch of samples, an internal rate of $125.58 or an external rate of $195.90 will be applied. This fee is only applied at a per-batch rate and is in addition to the sample fee.
$87.35
**Setup-fee:
$125.58 
$136.27
**Setup-fee:
$195.90
HETE/Prostaglandin Metabolites
Hydroxyicosatetraenoic acid (HETE) and Prostaglandin (PG) metabolite analysis by LC-MS – Species quantified include 5-HETE, 12-HETE, 15-HETE, 20-HETE, 5-HEPE, 12-HHT, 5-oxo-ETE, LTB4, 9-HODE, 13-HODE, PGE2, and PGD2. The following deuterated species are used as internal standards: d4-PGD2, d4-PGE2, d4-9-HODE, d4-13-HODE, d4-LTB4, d7-5-oxo-ETE, d8-5-HETE, d8-12-HETE, d8-15-HETE, and d8-20-HETE. Data is presented as the concentration of each metabolite normalized to tissue weight, total protein, or biofluid volume.
$70.78$110.42
Phospholipid Species
Phospholipid class composition analysis by LC-MS – Phospholipid species (PG, PE, PS, PI, PA, PC, SM, and LPC) are separated by LC-MS, and each fraction collected. Total phosphorus assay is performed on each phospholipid fraction. Data is presented as the mole percent of total phosphorus for each class.
$104.46$162.96 
Phospholipid Fatty Acid Species 
Quantification of phospholipid fatty acid species by LC-MS – Phospholipid species are separated by LC-MS, and each fraction collected. The individual fatty acid species are released by saponification, converted to their methyl esters with methanolic BF3, and then quantified by GC-MS. Species quantified include 12:0, 14:0, 14:1, 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 20:0, 20:1, 20:2, 20:3, 20:4, 20:5, 22:0, 22:1, 22:5, 22:6, 24:0, 24:5, and 24:6 phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA), phosphatidylserine (PS), phosphatidylinositol (PI), and phosphatidylglycerol (PG). di-15:0 LPC, and di-17:0 PG, PE, PC, PS, and PA are used as internal standards. Data is presented as the mole percent and concentration of each species normalized to total protein, tissue weight, or plasma volume.
$146.69$228.84 
Lysophospholipid Species
Quantification of lysophospholipid fatty acid species by LC-MS – Species are detected using a Thermo-Scientific TSQ Discovery Max. The individual species quantified include 12:0, 14:0, 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 20:0, 20:1, 20:2, 20:3, 20:4, 22:4, 22:5, 22:6, and 24:0 lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), lysophosphatidic acid (LPA), lysophosphatidylserine (LPS), lysophosphatidylinositol (LPI), and lysophosphatidylglycerol (LPG). d31-16:0 LPI, LPG, LPE, LPC, LPS, and LPA are used as internal standards. Data is presented as the mole percent and concentration of each species normalized to total protein or tissue weight.
$104.46$162.96 
Triglyceride Species 
Quantification of triglyceride fatty acid species by GC-MS – Triglycerides are isolated by solid phase extraction, the fatty acyl side chains released by saponification, converted to their methyl esters with methanolic BF3, and then detected using a Thermo-Scientific TSQ Quantum XLS GC-MS. The individual fatty acid species quantified include 14:0, 14:1, 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 18:3, 20:0, 20:1, 20:2, 20:3, 20:4, 20:5, 22:0, 22:1, 22:5, 22:6, 24:0, 24:1, 24:5, and 24:6. 15:0 and 21:0 FFAs, and 17:0-TG are used as internal standards. Data is presented as the concentration of each species normalized to total protein, tissue weight, or plasma volume, along with the mole and mass percent of each species. Minimum plasma volume required for analysis is 20 ul. For tissues and cells, minimum sample required will vary depending on the type of tissue or cells. 
$73.35 $114.43
Sphingosine-1-Phosphate
Quantification of total sphingosine-1-phosphate by LC-MS. C17-S1P is used as the internal standard. Data is presented as the concentration of total S1P, normalized to the tissue weight, total protein, or plasma volume extracted. Minimum plasma volume required for analysis is 25 ul. For tissues and cells, minimum sample required will vary depending on the type of tissue or cells.  
$70.68$110.26 
Proteomics ServiceFull Internal Rate
(per sample)*
External Rate
(per sample)
Protein Molecular Weight using Thermo Q-Exactive HF MS
Direct injection of intact protein for molecular weight determination.
$71.20$111.07
Protein Identification using Thermo Q-Exactive HF MS
Protein in-gel digestion, qualitative MS/MS analysis, and database search.
$190.70$297.49
Proteomics Method Development
Method development for SRM or PRM analysis of single target protein. 3 biological samples (cells or tissues) are required for analytical method setup. Cost will increase for multiple targets in a PRM analysis.
$1,131.80$1,765.61
Analysis of Post-translational Modifications (PTMs)
Protein in-gel/in-solution digestion, modified peptide enrichment, qualitative MS/MS analysis, and data processing for identification of PTMs. Peptides are enriched using various capturing materials such as titanium dioxide and immobilized metal affinity chromatography for phosphorylation. Costs may vary depending on the optional processes such as reversed-phase peptide fractionation prior to enrichment and the targeted PTM, which may require antibody-based enrichment or other strategies.
Phosphorylation: $302.96
- Oxidation: $395.40
- Glycation: $656.50
Phosphorylation: $469.59
- Oxidation: $612.87
- Glycation: $1,017.58 
Targeted Proteomics with SRM/PRM Analysis
Protein in-gel/in-solution digestion, quantitative MS/MS analysis, and data processing for relative quantitation. AQUA peptide(s) as internal standard(s) may be utilized if required, but with additional cost. Method setup described under "Proteomics Method Development" is required for SRM/PRM analysis.
$291.10 $454.12 
Peptide analysis with PRM
The service includes sample clean-up, instrument analysis, and data processing. AQUA peptide(s) as internal standard(s) may be utilized if required, but with additional cost.
$158.69$245.97
Relative Quantitative Analysis using SILAC
Protein in-gel/in-solution digestion, MS analysis, and data processing for relative quantitation. Workflow only applies to cell culture studies and does not include the cost of labeling the cells with heavy isotopes of aminoacids.
$1,333.30$2,079.95 
Relative Quantitative Analysis using Tandem Mass Tagging (TMT)
Tandem mass tagging of peptides for relative quantitation. Includes in-solution protein digestion, TMT labeling, peptide fractionation (10), qualitative MS/MS analysis, and data processing for search and relative quantitation. Thermo TMT isobaric labeling reagent is utilized. For extended numbers of samples, six-plex or ten-plex reagent kits may be utilized with additional charges. Please contact core staff for the extended multiplex analysis (e.g. six-plex, ten-plex).
$1,482.00$2,311.92 
Instrument Time (Q Exactive HF)
Instrument time for Q-Exactive HF per samples run. Peptides are separated on a Dionex Ultimate 3000 LC system equipped with PepMap C18 analytical column (75μm x 500mm) and PepMap C18 trap column (100μm x 2cm) with 90min gradient profile. Data acquisition method includes data-dependent acquisition (top-20), data-independent acquisition, single/parallel reaction monitoring for targeted omics and selected ion monitoring for parent MS observation.
$37.50$58.50 
Data Processing
Per hour cost for time spent on data analysis, data interpretation, and/or preparation of figures for publication. This service includes database search and data compilation for global protein identification using Proteome Discoverer and PEAKS. Data processes for label-free quantification, reporter ion quantification and SILAC quantification are also available. Sieve, Skyline and Spectronaut are utilized for targeted proteomics, such as single or parallel reaction monitoring (SRM/PRM) analyses.  Ingenuity Pathway Analysis is also available.
$50.20$78.31
MALDI-TOF Protein/Peptide Mass Analysis
Protein/Peptide molecular weight determination using MALDI-TOF.
$75.00$117.00
HPLC Service Full internal Rate (per sample)*External Rate 
(per sample) 
HPLC Setup Time 
Setup charge for HPLC analysis (required for all HPLC services). 
$100.00 $156.00
Automated HPLC
Automated analytical HPLC analysis.
[Columns: 2.1mm, 3.9mm, 4.6mm
Reverse Phase: C4, C8, C18, Size Exclusion (TSK) 2000,3000,4000
Automated Temperature control Column Heater
Automated Auto sampler 2-2000ul injections with temperature control from 4 degrees to 35 centigrade
Detection:UV/VIS; wavelength 190nm-700nm programmable channels along with multiple wavelengths at a time. Other detectors available: PDA, Fluorescence, and Wyatt: SEC-MALS, & QELS
$10.00$78.00
Manual HPLC from semi-micro to Preparative
Flow rates from 0.1mL/min to 100mL/min.
Columns: C4&C8 2.1mm; Ion exchange DEAE, MonoQ, MonoS, Poros R1/H;  Prep columns: Porasil [40x100]; RP C8 [10 x 100 ] & RP C4 [25x100, 40x100];C18 [8x100, 25x100, 40x100,
40x200mm] Detection via UV-Vis dual wavelength detector. Manual HPLC can range from 1ug  to purifying multi milligram quantities of protein.
Also, biologically active proteins can be purified in a all metallic free system.
$75.00$117.00
HPLC Gel Filtration
HPLC gel filtration analysis. Size Exclusion chromatography columns Superdex 200, & Sephacryl
S-300HR. TSK-2000, TSK3000, TSK4000SW
$200.00$312.00

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Quick Reference

Proteomics and Metabolomics Shared Resource
Director
Cristina M. Furdui, PhD

336-716-2697

cfurdui@wakehealth.edu

Co-Director
Mark Lively

336-716-2969

mlively@wakehealth.edu

Technical Staff
Anderson O'Brien Cox

aocox@wakehealth.edu

Technical Staff
Jingyun Lee

336-713-4194

jilee@wakehealth.edu

Technical Staff
Mark Morris

336-716-2581

jmmorris@wakehealth.edu

Locations
Hanes Bldg 2009/2029/2027
Medical Center Blvd. 
Winston-Salem, NC, 27157

Biotech Place
575 N Patterson Avenue
Winston-Salem, NC, 27101

How to Schedule Services
Please schedule services through iLab

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Last Updated: 01-12-2018
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