Proteomics and Metabolomics Shared Resource

Fee Schedule

Metabolomics Services and Fees
Proteomics Services and Fees 

*10% discount for CCC members

Metabolomics ServiceFull Internal Rate*External Rate 
Targeted Metabolomics Analysis (1 column)
Quantification of metabolites using a single chromatographic column. Minimum sample required for analysis is 5-10 mg for tissues, and 50-100 ul for biofluids. 
Metabolite List
$50.78$79.22
Targeted Metabolomics Analysis (2 columns)
Quantification of metabolites using two chromatographic columns. Minimum sample required for analysis is 5-10 mg for tissues, and 50-100 ul for biofluids. 
Metabolite List
$101.57$158.45
Pharmacokinetic Studies$57.23$89.28
Metabolomics Method Development 
Price for novel method development on a per hour basis.
$100.24$156.37
Metabolite and Other Small Molecule Molecular Weight Determination$35.00$54.60
Free Cholesterol (Less than 10 samples) $61.57$96.05
Free Cholesterol (10 or more samples) $44.23 $69.00
Total Cholesterol (Less than 10 samples)$61.57 $96.05
Total Cholesterol (10 or more samples) $44.23 $69.00
Cholesteryl Esters 
The individual cholesteryl ester species quantified include 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 20:3, 20:4, 20:5, and 22:6.  17:0 cholesteryl ester is used as an internal standard.  Data is presented as the mole percent and concentration of each species normalized to total protein, tissue weight, or plasma volume. 
$40.00 $62.40
Oxysterols (Less than 10 samples) 
The individual species quantified include 7α-hydroxycholesterol, 7β-hydroxycholesterol, 24- hydroxycholesterol, 25- hydroxycholesterol, 27- hydroxycholesterol, 7-ketocholesterol, 5α,6α-epoxycholestanol, 5β,6β-epoxycholestanol, 7α-OH-25-OH-cholesterol, 7β-OH-25-OH-cholesterol, and 7α-OH-27-OH-cholesterol. d7-24-hydroxycholesterol, d3-25-hydroxycholesterol, d7-7-ketocholesterol, and d5-27-OH-cholesterol are used as internal standards.
$83.46 $130.20
Oxysterols (10 or more samples) 
The individual species quantified include 7α-hydroxycholesterol, 7β-hydroxycholesterol, 24- hydroxycholesterol, 25- hydroxycholesterol, 27- hydroxycholesterol, 7-ketocholesterol, 5α,6α-epoxycholestanol, 5β,6β-epoxycholestanol, 7α-OH-25-OH-cholesterol, 7β-OH-25-OH-cholesterol, and 7α-OH-27-OH-cholesterol. d7-24-hydroxycholesterol, d3-25-hydroxycholesterol, d7-7-ketocholesterol, and d5-27-OH-cholesterol are used as internal standards.
$51.23 $79.92
Free Fatty Acids (Less than 10 samples) 
The individual fatty acid species quantified include 14:0, 14:1, 16:0, 16:1, 18:0, 18:1 (oleate and vaccinate), 18:2(ω6), 18:3(ω3), 18:3(ω6), 20:0, 20:1, 20:2(ω6), 20:3(ω3), 20:4(ω6), 20:5(ω3), 22:0, 22:1, 22:5(ω3), 22:6(ω3), 24:0, 24:1, 24:5(ω3), and 24:6(ω3). 15:0 and 21:0 are used as internal standards. Measurement of additional species, such as 6:0, 8:0, etc., are available upon request only.
$63.57 $99.17 
Free Fatty Acids (10 or more samples) 
The individual fatty acid species quantified include 14:0, 14:1, 16:0, 16:1, 18:0, 18:1 (oleate and vaccinate), 18:2(ω6), 18:3(ω3), 18:3(ω6), 20:0, 20:1, 20:2(ω6), 20:3(ω3), 20:4(ω6), 20:5(ω3), 22:0, 22:1, 22:5(ω3), 22:6(ω3), 24:0, 24:1, 24:5(ω3), and 24:6(ω3). 15:0 and 21:0 are used as internal standards. Measurement of additional species, such as 6:0, 8:0, etc., are available upon request only.
$44.23 $69.00 
Total Fatty Acids (Less than 10 samples)
The individual fatty acid species quantified include 14:0, 14:1, 16:0, 16:1, 18:0, 18:1 (oleate and vaccinate), 18:2(ω6), 18:3(ω3), 18:3(ω6), 20:0, 20:1, 20:2(ω6), 20:3(ω3), 20:4(ω6), 20:5(ω3), 22:0, 22:1, 22:5(ω3), 22:6(ω3), 24:0, 24:1, 24:5(ω3), and 24:6(ω3). 15:0 and 21:0 are used as internal standards. Measurement of additional species, such as 6:0, 8:0, etc., are available upon request only.
$61.57$96.05 
Total Fatty Acids (10 or more samples)
The individual fatty acid species quantified include 14:0, 14:1, 16:0, 16:1, 18:0, 18:1 (oleate and vaccinate), 18:2(ω6), 18:3(ω3), 18:3(ω6), 20:0, 20:1, 20:2(ω6), 20:3(ω3), 20:4(ω6), 20:5(ω3), 22:0, 22:1, 22:5(ω3), 22:6(ω3), 24:0, 24:1, 24:5(ω3), and 24:6(ω3). 15:0 and 21:0 are used as internal standards. Measurement of additional species, such as 6:0, 8:0, etc., are available upon request only.
$42.23 $65.88
HETE/Prostaglandin Metabolites
Hydroxyicosatetraenoic acid (HETE) and Prostaglandin (PG) metabolite analysis by LC-MS – Metabolites are detected using a Thermo-Scientific TSQ Discovery Max LC-MS. Species quantified include 5-HETE, 12-HETE, 15-HETE, 20-HETE, 5-HEPE, 12-HHT, 5-oxo-ETE, LTB4, 9-HODE, 13-HODE, PGE2, and PGD2. The following deuterated species are used as internal standards: d4-PGD2, d4-PGE2, d4-9-HODE, d4-13-HODE, d4-LTB4, d7-5-oxo-ETE, d8-5-HETE, d8-12-HETE, d8-15-HETE, and d8-20-HETE. 
$70.78$110.42 
Phospholipid Species
Phospholipid class composition analysis by LC-MS – Phospholipid species (PG, PE, PS, PI, PA, PC, SM, and LPC) are separated by LC-MS, and each fraction collected. Total phosphorus assay is performed on each phospholipid fraction. Data is presented as the mole percent of total phosphorus for each class.
$104.46$162.96 
Phospholipid Fatty Acid Species
Phospholipid species are separated by LC-MS, and each fraction collected. The individual fatty acid species are released by saponification, converted to their methyl esters with methanolic BF3, and then quantified by GC-MS. Species quantified include 12:0, 14:0, 14:1, 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 20:0, 20:1, 20:2, 20:3, 20:4, 20:5, 22:0, 22:1, 22:5, 22:6, 24:0, 24:5, and 24:6 phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA), phosphatidylserine (PS), phosphatidylinositol (PI), and phosphatidylglycerol (PG). di-15:0 LPC, and di-17:0 PG, PE, PC, PS, and PA are used as internal standards.
$146.69 $228.84 
Lysophospholipid Species
The individual species quantified include 12:0, 14:0, 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 20:0, 20:1, 20:2, 20:3, 20:4, 22:4, 22:5, 22:6, and 24:0 lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), lysophosphatidic acid (LPA), lysophosphatidylserine (LPS), lysophosphatidylinositol (LPI), and lysophosphatidylglycerol (LPG). d31-16:0 LPI, LPG, LPE, LPC, LPS, and LPA are used as internal standards.
$104.46 $162.96 
Triglyceride Species
The individual fatty acid species quantified include 14:0, 14:1, 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 18:3, 20:0, 20:1, 20:2, 20:3, 20:4, 20:5, 22:0, 22:1, 22:5, 22:6, 24:0, 24:1, 24:5, and 24:6.
$73.35 $114.43 
Ceramide Species Analysis
The individual species quantified include ceramides 14:0, 14:1, 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 20:0, 20:1, 20:2, 20:3, 20:4, 20:5, 22:0, 22:1, 22:2, 22:5, 22:6, 24:0, 24:1, 24:5, and 24:6.
$57.23 $89.28 
Sphingosine-1-Phosphate$70.68 $110.26 
F2-Isoprostanes
Total isoprostanes analysis by GC-MS. d4-PGF2α is used as the internal standard. Data is presented as the concentration of PGF2α, and total isoprostanes, normalized to tissue weight, total protein, or plasma volume.
$105.58 $164.70 
Proteomics ServiceFull Internal Rate*External Rate
Protein Molecular Weight using Thermo Q-Exactive HF MS$71.20$111.07
Protein Identification using Thermo Q-Exactive HF MS
Protein in-gel digestion, qualitative MS/MS analysis, and database search.
$190.70$297.49
Proteomics Method Development
Method development for SRM analysis of single target protein. 3 biological samples (cells or tissues) are required for analytical method setup. Cost will increase for multiple targets in a PRM analysis.
$1,131.80$1,765.61
Analysis of Post-translational Modifications (PTMs)
Protein in-gel/in-solution digestion, modified peptide enrichment, qualitative MS/MS analysis, and data processing for identification of PTMs. 
$371.04$578.82 
Targeted Proteomics with SRM/PRM Analysis
Protein in-gel/in-solution digestion, quantitative MS/MS analysis, and data processing for relative quantitation. AQUA peptide(s) as internal standard(s) may be utilized if required, but with additional cost. 
$291.10 $454.12 
Relative Quantitative Analysis using SILAC
Protein in-gel/in-solution digestion, MS analysis, and data processing for relative quantitation. Workflow only applies to cell culture studies.
$1,333.30$2,079.95 
Relative Quantitative Analysis using Tandem Mass Tagging (TMT)
Tandem mass tagging of peptides for relative quantitation. Includes in-solution protein digestion, TMT labeling, peptide fractionation (10), qualitative MS/MS analysis, and data processing for search and relative quantitation. Thermo TMT isobaric labeling reagent is utilized. 
$1,482.00$2,311.92 
Data Processing
Per hour cost for time spent on data analysis, data interpretation, and/or figure construction. This service includes database search and data compilation for global protein identification using Proteome Discoverer and PEAKS. Data processes for label-free quantification, reporter ion quantification and SILAC quantification are also available. Sieve, Skyline and Spectronaut are utilized for targeted proteomics, such as single or parallel reaction monitoring (SRM/PRM) analyses.  Ingenuity Pathway Analysis is also available.
$50.20 $78.31 
MALDI-TOF Protein/Peptide Mass Analysis$75.00$117.00 
Protein Sequencing (1-5 amino acids)
Protein sequencing using Edman degradation (first five amino acid residues).
$200.00 $312.00 
Protein Sequencing (>5 amino acids)
Protein sequencing using Edman degradation (each additional amino acid residue after the 5th).
$30.00 $46.80
HPLC Setup Time
Setup charge for HPLC analysis (required for all HPLC services).
$100.00 $78.00 
Automated HPLC
Automated analytical HPLC analysis.   [Columns: 2.1mm, 3.9mm, 4.6m; Reverse Phase: C4, C8, C18; Size Exclusion (TSK) 2000,3000,4000] Detection:UV/VIS; wavelength 190nm-700nm programmable channels along with multiple wavelengths at a time. Other detectors available: PDA, Fluorescence, and Wyatt: SEC-MALS, & QELS
$50.00 $78.00 
Manual HPLC from semi-micro to Preparative
Manual analytical HPLC analysis. [Columns: C4&C8 2.1mm; Ion exchange DEAE, MonoQ, MonoS, Poros R1/H;  Prep columns: Porasil [40x100]; RP C8 [10 x 100 ] & RP C4 [25x100, 40x100];C18 [8x100, 25x100, 40x100, 40x200mm] Detection via UV-Vis dual wavelength detector. Metallic free system available for purifying biologically active proteins.
$75.00$117.00 
HPLC Gel Filtration
HPLC gel filtration analysis. Size Exclusion chromatography columns Superdex 200, & Sephacryl
S-300HR. TSK-2000, TSK3000, TSK4000SW
$200.00 $312.00 

*10% Discount for CCC Members

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Quick Reference

Proteomics and Metabolomics Shared Resource
Director
Cristina M. Furdui, PhD

336-716-2697

cfurdui@wakehealth.edu

Co-Director
Mark Lively

336-716-2969

mlively@wakehealth.edu

Technical Staff
Anderson O'Brien Cox

aocox@wakehealth.edu

Technical Staff
Jingyun Lee

336-713-4194

jilee@wakehealth.edu

Technical Staff
Mark Morris

336-716-2581

jmmorris@wakehealth.edu

Locations
Hanes Bldg 2009/2029/2027
Medical Center Blvd. 
Winston-Salem, NC, 27157

Biotech Place
575 N Patterson Avenue
Winston-Salem, NC, 27101

How to Schedule Services
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