Proteomics and Metabolomics Shared Resource

Services We Provide

Equipment and Applications

  • Thermo-Scientific Q-Exactive HF mass spectrometer with Dionex nanoLC system and auto-sampler.  This instrument is used for proteomics studies, including protein identification, SILAC, post-translational modification analysis, targetd SRM/PRM analysis, and tandem mass tagging of peptides for relative quantitation.
    Q-Exactive
  • Shimazdu LCMS-8050 triple quadrupole mass spectrometer with Shimazdu UFLC system and auto-sampler.  This instrument is used for targeted metabolomics and pharmacokinetic studies.  Examples of metabolite classes measured include, amino acids, nucleotides, sphingolipids, organic acids, etc. Please see the Metabolites List for a list of metabolites included in a typical targeted metabolomics analysis. 
    Shimazdu LCMS8050
  • Thermo Scientific TSQ Quantum XLS GC/MS with Trace Ultra gas chromatograph, and Triplus auto-sampler. This instrument is used for analysis of fatty acid methyl esters, free and total cholesterol, oxysterols, triglycerides, and isoprostanes.
    TSQ Quantum XLS
  • Thermo Scientific TSQ Quantum Discovery Max with HP/Agilent 1100 HPLC and HP/Agilent auto-sampler. This instrument is used for analysis of phospholipids (PC, PE, PG, PI, PS, PA, LPC, and Sphingomyelin), Sphingosine-1-Phosphate, FTY-720, cholesteryl esters, triglycerides, coumarin/diazepam metabolites, halofuginone, and polyunsaturated fatty acids.
    TSQ Discovery Max
  • Analytical HPLC of Biomolecules: Columns: 2.1mm, 3.9mm, 4.6mm; C4, C18; Size Exclusion (TSK) 2000,3000,4000. Automated temperature control column heater.  Auto-sampler capable of 2-2000ul injections with temperature control from 4 degrees to 35 centigrade. Variable wavelength (190nm-700nm) programmable channels along with multiple wavelengths at a time.
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  • Waters 650E HPLC & LC150 Ssystem for protein purification. Flow rates ranging from 0.1mL/min to 100mL/min. Columns: Ion exchange [DEAE, MonoQ, MonoS, Poros R1/H; Size Exclusion chromatography columns Superdex 200, & Sephacryl S-300HR. Prep columns: Porasil [40x100]; RP C8 [10 x 100 ] & RP C4 [25x100, 40x100];C18 [8x100, 25x100, 40x100, 40x200mm].  Detection via UV-Vis dual wavelength detector.  Investigators can use this system to purify multi-milligram quantities of biologically active proteins.
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  • Bruker Auto-flex MALDI-TOF.  A time-of-flight mass spectrometer with high sensitivity and mass accuracy. The instrument is used for accurate mass measurement of intact proteins up to 100 kDa.
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  • Protein and peptide sequence analyses are performed on a Perkin Elmer/Applied Biosystems Model 492HT Procise automated protein sequencer. Sequence data can be obtained with a detection limit of approximately 0.5 to 1 pmole of protein. The protein sequencer yields N-terminal sequence data from purified proteins and peptides as well as by transfer blotting from PVDF membranes following electrophoresis (1-D or 2-D). Expected sequence results can be obtained up to about 30 amino acids from the n-terminus of a highly purified protein or peptide.
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For other broad and targeted metabolomics studies the Core partners with RTI Metabolomics, a Regional Comprehensive Metabolomics Resource Core, funded by the National Institutes of Health (NIH) Common Fund. The RTI Metabolomics team is directed by Dr. Susan Sumner. Please contact Dr. Furdui for further information. 

How to Schedule Services

Please schedule services through iLab.

 

Quick Reference

Proteomics and Metabolomics Shared Resource
Director
Cristina M. Furdui, PhD

336-716-2697

cfurdui@wakehealth.edu

Co-Director
Mark Lively

336-716-2969

mlively@wakehealth.edu

Technical Staff
Anderson O'Brien Cox

aocox@wakehealth.edu

Technical Staff
Jingyun Lee

336-713-4194

jilee@wakehealth.edu

Technical Staff
Mark Morris

336-716-2581

jmmorris@wakehealth.edu

Locations
Hanes Bldg 2009/2029/2027
Medical Center Blvd. 
Winston-Salem, NC, 27157

Biotech Place
575 N Patterson Avenue
Winston-Salem, NC, 27101

How to Schedule Services
Please schedule services through iLab

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Last Updated: 08-05-2016
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