Lipoprotein Analysis Laboratory

Laboratory Purpose:

To provide analytical and quantitative measurements of lipid and lipoprotein endpoints for non-diagnostic investigational purposes.

Capabilities:

• Quantitative measurements of apolipoproteins AI, AII, B, E, and Lp(a) by ELISA
• Lipoprotein fractionation and estimation of LDL particle size
• Lipoprotein-cholesterol distribution in plasma
• Quantitative measurements of fatty acids by GC
• Quantitative measurements of cholesterol and plant sterols by GC

Instrumentation:

• Agilent 6890N GC systems with autosamplers (2)
• Beckman Optima MAX-E Ultracentrifuge with TLA 55, TLA 120.1 and TLA 120.2 rotors
• Beckman L8-70 Ultracentrifuge and 70.1 Ti rotor
• Beckman System Gold HPLC with autosampler, PDA detector, ELSD detector
• Hitachi LaChrom Elite HPLC systems with Peltier cooled autosamplers (2)
• Wyatt DAWN HELEOS-II light scattering detector with QELS
• Wyatt Optilab rEX refractive index detector
• Tecan GENios absorbance, fluorescence, and glow type luminescence platereader

Contact Information:

Telephone Number: 336-716-2137

Director: Lawrence Rudel, PhD
Tel: 336-716-2821
Fax: 336-716-6279
lrudel@wakehealth.edu

Supervisor: Martha Wilson, PhD
Tel: 336-716-2137
Fax: 336-716-6279
mdwilson@wakehealth.edu