Madras Veterinary College, 2005
Michigan State University, 2011
University of Michigan Medical School, 2015
Urinary tract infection, Inflammation, Metals at host-pathogen interface
urinary tract infection (UTI) is a major global public health problem and uropathogenic
Escherichia coli (UPEC) is the
predominant causative agent of UTI. Annual estimates for the incidence of UTI
are ~11 million and ~150 million in the United States and around the world,
respectively. High incidence of UTI and an alarming increase in antibiotic
resistant uropathogens underscore the immediate need for development of novel
strategies to mitigate UTI. We study the fitness and virulence mechanisms
involved in the pathogenesis of bacterial UTI to identify novel targets that
could be harnessed to develop therapeutics or prevention strategies against
this ubiquitous infectious condition.
use a combination of bacteriological, biochemical, genetic, and functional
genomic approaches, in conjunction with a mouse model, and ex vivo and in vitro
models to study key aspects of host-uropathogen interaction during UTI. Ongoing
projects in the laboratory include elucidating the role of copper in UPEC
survival and virulence during UTI, identification of small molecule inhibitors
of UPEC virulence, developing a bladder organoid model to study the molecular
pathogenesis of UTI in vitro, and
investigating the biology of gut colonization by UPEC.
Subashchandrabose S, Smith SN, DeOrnellas V, Crepin S,
Zahdeh C, Kole MM, Mobley HL. 2015. Acinetobacter baumannii Genes
Required for Bacterial Survival During Bloodstream Infection. mSphere 00013-15R1. In Press.
Subashchandrabose S and Mobley HL. 2015. Back to the
Metal Age: Battle for Metals at the Host-Pathogen Interface During Urinary Tract
Infection. Metallomics. doi: 10.1039/C4MT00329B (Review).
Smith SN, Subashchandrabose S,
Himpsl SD, Hazen TH, Rasko DA, Mobley HL. 2015. Blocking Yersiniabactin
Attenuates Extraintestinal Pathogenic Escherichia
coli in Cystitis and Pyelonephritis and is a Novel Target to Prevent
Urinary Tract Infection. Infect. Immun. doi: 10.1128/IAI.02904-14
Subashchandrabose S and Mobley HL. 2015. Virulence and
Fitness Determinants of Uropathogenic E.
coli. Microbiology Spectrum. doi: 10.1128/microbiolspec.UTI-0015-2012
*Subashchandrabose S, Hazen TH, Brumbaugh AR, Himpsl SD,
Smith SN, Ernst RD, Rasko DA, Mobley HL. 2014. Host-specific Induction
of Escherichia coli Fitness Genes During Human Urinary Tract Infection. Proc. Natl. Acad. Sci. USA.
in Nat. Rev. Urology. doi:10.1038/nrurol.2014.366.
Subashchandrabose S, Smith SN, Spurbeck RR, Kole MM,
Mobley HL. 2013. Genome-wide Detection of Fitness Genes in Uropathogenic Escherichia coli During Systemic
Infection. PLoS Pathog. doi:
S, Hazen TH, Rasko
DA, Mobley HL. 2013. Draft
Genome Sequences of Five Recent Human Uropathogenic Escherichia coli Isolates.Pathog. Dis.
Subashchandrabose S, Leveque RM, Kirkwood RN, Kiupel M,
Mulks MH. 2013. The RNA Chaperone Hfq Promotes Fitness of Actinobacillus pleuropneumoniae During Porcine Pleuropneumonia. Infect. Immun.
Subashchandrabose S, Leveque RM, Kirkwood RN,
Kiupel M, Mulks MH. 2009. Branched-chain Amino Acids are Required for the
Survival and Virulence of Actinobacillus
pleuropneumoniae in Swine. Infect. Immun. doi: