Meet Our Pre-doctoral Fellows

Renata Magalhaes, MD 
(Mentors: K. Williams, DVM and A. Atala, MD; Appointment: November 2016-Present)

Renata MagahaesRenata Magalhaes is a 3rd year Integrative Physiology and Pharmacology graduate student at the Wake Forest School of Medicine. Renata received her medical degree from the Pontificia Universidade Catolica de Sao Paulo, Brazil in 2002 and successfully completed her residency in Obstetrics, Gynecology, and fellowship in Minimally Invasive Surgery in 2006 at the Faculdade de Medicina do ABC, Brazil. In 2012 she joined Dr Atala’s research team and has been involved in translational projects pertaining the female reproductive system.
Renata’s research project focuses on using tissue-engineering technologies to develop functional lab grown uterine tissue as a potential treatment for women with uterine factor infertility. She is currently working on bioengineering autologous uterine tissue in a rabbit model.

Publications:

Zambon JP, Magalhaes RS, Ko I, Ross CL, Orlando G, Peloso A, Atala A, Yoo JJ. Kidney regeneration: where we are and future perspectives. World J Nephrol. 2014; 3(3): 24-30.

Zambon JP, Magalhaes RS, Almeida FG. Stress urinary incontinence in women and cell therapy: what can we expect from the future. World J Clin Urol. 2014; 3(3): 304-09.

Zambon JP, de Sa Barretto LS, Nakamura AN, Duailibi S, Leite K, Magalhaes RS, Orlando G, Ross CL, Peloso A, Almeida FG. Histological changes induced by Polyglycolic-Acid (PGA) scaffolds seeded with autologous adipose or muscle-derived stem cells when implanted on rabbit bladder. Organogenesis. 2014; 10(2): 278-288.

Poster Presentations:

WFIRM Annual Retreat Pinehurst, NC, February 2016

Renata S. Magalhaes, James K. Williams, Ashley S. Dean, Shannon Lankford, Tammy J. Cockerham, Kimberly Poppante, James Yoo, and Anthony Atala. Successful Conception and Live Birth from a Tissue Engineered Uterus in a Rabbit Model
Preliminary Results.

Wake Forest University Graduate Student and Postdoctoral Research Day, Winston-Salem, NC, March 2016

Renata S. Magalhaes, James K. Williams, Ashley S. Dean, Shannon Lankford, Tammy J. Cockerham, Kimberly Poppante, James Yoo, and Anthony Atala. Successful Conception and Live Birth from a Tissue Engineered Uterus in a Rabbit Model
Preliminary Results.

Wake Forest School of Medicine Division of Surgical Sciences Research Day, Winston-Salem, NC, Novmber 2016

Renata S. Magalhaes, James K. Williams, Ashley S. Dean, Shannon Lankford, Tammy J. Cockerham, Kimberly Poppante, James Yoo, and Anthony Atala. Successful Conception and Live Birth from a Tissue Engineered Uterus in a Rabbit Model
Preliminary Results.

Oral Presentation:

WFIRM Annual Retreat Pinehurst, NC, February 2016.

Renata S. Magalhaes, James K. Williams, Ashley S. Dean, Shannon Lankford, Tammy J. Cockerham, Kimberly Poppante, James Yoo, and Anthony Atala. Successful Conception and Live Birth from a Tissue Engineered Uterus in a Rabbit Model
Preliminary Results.

Awards:

First Place Oral Presentation at Wake Forest Institute for Regenerative Medicine Annual Retreat, Pinehurst, NC (February 2016).

Second Place Overall Poster Award at Wake Forest Institute for Regenerative Medicine Annual Retreat, Poster Session, Pinehurst, NC (February 2016).

Student Gold Award in Basic Science - Wake Forest School of Medicine Division of Surgical Sciences Research Day (November 2016)

 

Matthew and Mahesh
Matthew Brovold (Left) and Mahesh Devarasetty (Right)


Matthew Brovold
(Mentor: Shay Soker, PhD; Appointment: October 2015-Present)

Matthew Brovold is a 3rd year Molecular Medicine and Translation Science graduate student at the Wake Forest School of Medicine.  Matthew received his Bachelor’s in Genetics, Molecular and Cellular Biology in 2011 and worked as a Research Technician in 2012 before joining Dr. Shay Soker’s research group in 2013. Matthew’s research centers on fibrotic diseases of the fetal liver and their effect on organogenesis.  Currently he is investigating the role of Hepatic Stellate Cells during liver during this critical process by the development of an in vitro modeling system.  This system will also be used to study a variety of congenital liver diseases.

Publications:

Kong, W., M. Brovold, B. Koeneman, J. Clark-Curtiss, and R. Curtiss III. Turning self-destructing Salmonella into a universal DNA vaccine delivering platform. Published November 5, 2012, doi: 10.1073/pnas.1217554109 Proc. Natl. Acad. Sci. USA. * This article has been recommended by Faculty of 1000.

Poster Presentations:

G. Llamazares, M. Brovold (Presenter), R. Monge, S. Mokhtari, D. Izquierdo, F. Sotelo, A. Argues, J. Ayuso, A. Vigueras, J Santolaria, I. Garces, G. Alameida-Porada, I. Ochoa, L. Fernendez, S. Soker. Microreactor/Microfluidic devices for non-invasive and real time monitoring of oxygen and transepithelial electrical resistance.  NCTERMS, 2015, Winston-Salem, NC

G. Llamazares, M. Brovold (Presenter), R. Monge, S. Mokhtari, D. Izquierdo, F. Sotelo, A. Argues, J. Ayuso, A. Vigueras, J Santolaria, I. Garces, G. Alameida-Porada, I. Ochoa, L. Fernendez, S. Soker. Microreactor/Microfluidic devices for non-invasive and real time monitoring of oxygen and transepithelial electrical resistance.  2016 Graduate Student and Postdoc Research Day, Winston-Salem, NC, March 24, 2016 at WF Biotech Place Conference Center

Brovold, M. (Presenter), D. Vyas, E. Moran, P. Baptista, S. Soker.  The roles of LGR5, WNT, and Notch signaling in human fetal liver stem cell proliferation and differentiation, 2015 WFIRM annual retreat, Pinehurst, NC

Brovold, M. (Presenter), D. Vyas, E. Moran, P. Baptista, S. Soker.  The roles of LGR5, WNT, and Notch signaling in human fetal liver stem cell proliferation and differentiation, 2015 Graduate Student and Postdoc Research Day, Winston-Salem, NC

Brovold, M. (Presenter), D. Vyas, E. Moran, P. Baptista, S. Soker.  The roles of LGR5, WNT, and Notch signaling in human fetal liver stem cell proliferation and differentiation, 2014 NCTERMS, Duke University, Durham, NC

Submitted papers:

Dipen Vyas, Pedro M. Baptista, Tommi Talheden, Emma Moran, Matthew Brovold, Brandon Gaston, Chris Booth, Michael Samuel, Mark Furth, Anthony Atala, and Shay Soker.

Self-assembled liver organoids recapitulate hepato-biliary organogenesis in vitro

Additional collaborations:

Guillermo Llamazares, University of Zaragoza, Zaragoza
This collaboration purpose is toward the development of microfluidic device for the use in culturing human fetal liver stem cells.

University of Southern California, Los Angeles, CA.  The collaboration is based upon utilizing decellularized liver matrix technology as a platform for development and validation of the computer modeling of the effects of the physical environment on the cancer growth and metastasis.


Mahesh Devarasetty
(Mentors: Shay Soker, PhD and Aleks Skardal; Appointment: October 2015-Present)

Mahesh is a 3rd year biomedical engineering student in the Virginia Tech – Wake Forest School for Biomedical Engineering and Sciences. He received his B.S. in biomedical engineering from Columbia University, graduating in 2012.  Mahesh’s research deals with the construction and testing of various microscale tissue models.  He is an active member of the XCEL body-on-a-chip project, utilizing his engineering experience to develop microfluidic and sensor technologies.  His future goals include using microfabricated models, or organs-on-a-chip, for personalized medicine and drug screening.  Mahesh’s project revolves around building a model of the colon for use in colorectal cancer modeling. Our model is based around the interaction of the submucosa and the epithelium of the colon, the initiating site of many colorectal cancers.  Using this colonic model, we’ve found a link between mechanical properties (such as stiffness and fiber alignment) and cellular processes (Focal adhesion kinase activation and downstream WNT activity).

Publications:

Skardal A, Devarasetty M, Forsythe S, Atala A, Soker S. A reductionist metastasis-on-a-chip platform for in vitro tumor progression modeling and drug screening. Biotechnology and bioengineering. Feb 16 2016. 

Skardal A, Devarasetty M, Kang H-W, et al. A hydrogel bioink toolkit for mimicking native tissue biochemical and mechanical properties in bioprinted tissue constructs. Acta Biomaterialia. 2015. 

Skardal A, Devarasetty M, Soker S, Hall AR. In situ patterned micro 3D liver constructs for parallel toxicology testing in a fluidic device. Biofabrication. 2015;7(3):031001.

Skardal A, Devarasetty M, Rodman C, Atala A, Soker S. Liver-Tumor Hybrid Organoids for Modeling Tumor Growth and Drug Response In Vitro. Annals of biomedical engineering. Mar 17 2015.

Poster Presentations:

WFIRM Annual Retreat | February 2nd, 2015

Modeling the intestine-tumor microenvironment using multicellular hydrogel strata. Mahesh Devarasetty, Aleksander Skardal, and Shay Soker

Wake Forest University Graduate Student and Postdoctoral Research Day | March 20th, 2015

Modeling the intestine-tumor microenvironment using multicellular hydrogel strata. Mahesh Devarasetty, Aleksander Skardal, and Shay Soker

In Situ Patterned Micro 3-D Liver Constructs for Parallel Toxicology Testing in a Fluidic Device. Aleksander Skardal. Mahesh Devarasetty, Shay Soker, and Adam Hall

A Hydrogel Bioink Toolbox for Mimicking Native Tissue Biochemical and Mechanical Properties. Aleksander Skardal, Mahesh Devarasetty, Hyun-Wook Kang, Ivy Mead, Colin Bishop, Thomas Shupe, Sang Jin Lee, John Jackson, James Yoo, Shay Soker, and Anthony Atala.

Integration of 3-D Organoid Bioprinting and Microfluidic Device Technology for Functional Primary Cell-Based Liver-on-a-Chip Operation. Aleksander Skardal, Ivy Mead, Mahesh Devarasetty, Hyun-wook Kang, Thomas Shupe, San-jin Lee, John Jackson, Colin Bishop, James Yoo, Shay Soker, and Anthony Atala

Functionalized Alginate Hydrogels for 3-D Cell Culture and Biofabrication. Steven Forsythe, Mahesh Devarasetty, Yuanyuan Zhang, and Aleksander Skardal

WFU-VT SBES Symposium | May 13th, 2015

A 3D Stratified Colon Model for Colorectal Cancer Progression. Mahesh Devarasetty, Aleksander Skardal, and Shay Soker

TERMIS-WC | September 9th, 2015

A 3D Stratified Colon Model for Colorectal Cancer Progression. Mahesh Devarasetty, Aleksander Skardal, and Shay Soker

A Hydrogel Bioink Toolkit for Bioprinting Tissue Constructs while Mimicking Native Tissue Biochemical and Mechanical Properties. Aleksander Skardal, Mahesh Devarasetty, Hyun-Wook Kang, Ivy Mead, Colin Bishop, Thomas Shupe, Sang Jin Lee, John Jackson, James Yoo, Shay Soker, and Anthony Atala.

Ultra-thin Tissue-Tumor Organoid Microfluidic Platform for In Vitro Diagnostics. Mahesh Devarasetty, Steven Forsythe, Shay Soker, Adam Hall, and Aleksander Skardal.

Physiologically Relevant Drug Testing In Vitro - An Integrated Multiple Organoid-on-a-Chip Approach. Aleksander Skardal, Andre Kleensang, Mahesh Devarasetty, Hyun-Wook Kang, Ivy Mea , Colin Bishop, Thomas Shupe, San Jin Lee, John Jackson, James Yoo , Thomas Hartung, Shay Soker, and Anthony Atala.

BMES Annual Meeting | October 9th, 2015

A 3D Stratified Colon Model for Colorectal Cancer Progression. Mahesh Devarasetty, Aleksander Skardal, and Shay Soker

A Metastasis-on-a-Chip System for Modeling Colon Carcinoma Migration and Invasion In Vitro. Mahesh Devarasetty, Anthony Atala, Shay Soker, and Aleksander Skardal.

A 3-D Primary Hepatocyte and Tumor Organoids Platform for Metastasis Research and Drug Screening. Edina Wang, Mahesh Devarasetty, Shay Soker, and Aleksander Skardal.

NCTERMS | October 16th, 2015

Physiologically Relevant Drug Testing In Vitro - An Integrated Multiple Organoid-on-a-Chip Approach. Aleksander Skardal, Andre Kleensang, Mahesh Devarasetty, Hyun-Wook Kang, Ivy Mea , Colin Bishop, Thomas Shupe, San Jin Lee, John Jackson, James Yoo , Thomas Hartung, Shay Soker, and Anthony Atala.

Modeling the intestine-tumor microenvironment using multicellular hydrogel strata. Mahesh Devarasetty, Aleksander Skardal, and Shay Soker

Oral Presentations

WFIRM Retreat | 2016 | A 3D Submucosal Microenvironment for Investigation of Fiber Alignment Induced Epithelial-to-Mesenchymal Transition in Colorectal Cancer Cells

Additional Collaborations

MIT/Harvard – Khademhosseini Lab – Development of microfluidic devices for a multi-scale body-on-a-chip system for the XCEL program. Collaboration on an image based quantification system for cardiac functionality.

WFIRM – Regenerative Medicine Clinical Translational Center– Collaborative design of a 3D printed bioreactor holder and stand.

WFIRM – Frank Marini Lab – Collaboration on the imaging and quantification of fiber orientation and alignment in cell-organized collagen constructs.


Amritha Kidiyoor 
(Mentors: Anthony Atala; Appointment: September 2015-Present)

Amritha Kidiyoor Web

Amritha Kidiyoor is a 3rd year Molecular Medicine and Translation Science graduate student at the Wake Forest School of Medicine. Amritha received her Bachelor’s in Biotechnology Engineering in 2008 and her M.S in Biology in 2012.  Amritha’s research project focuses on using reprogramming technology to convert damaged fibrotic lung tissue into functional lung tissue as a potential treatment for pulmonary fibrosis. She is currently investigating the reprogramming capacity of specific genes in different combinations on lung cells.

Kidiyoor A., Murphy S.V., Atala A. ‘Stem Cells from the Amniotic fluid and Placenta’ in Cell and Molecular Biology and Imaging of Stem Cells, 2014

Kidiyoor A., Murphy S.V., Atala A.  ‘Adult Lung Stem Cells’ in Gene and Cell Therapy, 2014

Murphy S.V., Kidiyoor A., Reid T., Atala A., Wallace E.M., Lim R. ‘Isolation, cryopreservation and culture of human amnion epithelial cells for clinical applications’ in JOVE, 2014 

Amritha Kidiyoor, Jorge Schettini*, Dahlia M. Besmer*, Stephen Rego, Sritama Nath, Jennifer M. Curry, Lopamudra Das Roy, Didier Dreau and Pinku Mukherjee Pancreatic Cancer Cells Isolated from Muc1-Null Tumors Favor the Generation of a Mature Less Suppressive MDSC Population. Frontiers Immunology, 2014 *Both authors contributed equally

Poster presentations:

WFIRM Retreat February 2016
Amritha Kidiyoor, Sean V Murphy and Anthony J Atala. Characterizing embryonic stem cell derived multipotent lung stem cells.

Gordon Conference on Lung Development, Injury & Repair, 2015
Amritha Kidiyoor, Sean V Murphy and Anthony J Atala. Characterizing embryonic stem cell derived multipotent lung stem cells.

NC TERMS October 2015
Amritha Kidiyoor, Sean V Murphy and Anthony J Atala. Characterizing embryonic stem cell derived multipotent lung stem cells.

WFIRM Retreat February 2015
Amritha Kidiyoor, Sean V Murphy and Anthony J Atala. Restoring lung function in damaged lung.

Oral presentations:

Annual Pulmonary Horizons COPD conference as invited Keynote speaker, Miami FL, July 2015.
Amritha Kidiyoor, Sean Murphy and Anthony Atala. Regenerative Medicine Technologies for the treatment of lung disease.

Additional Collaborations

UNC Chapel Hill, Cystic Fibrosis and Pulmonary Diseases Research and Treatment Center: Growth and characterization of lung epithelial cells

TherimuneX Pharmaceuticals: Determination of their novel drug in our pulmonary fibrosis mouse model 

 

Ashley Wagoner
(Mentor: Stephen J. Walker, PhD; Appointment: April 2015-Present) 

Ashley Wagoner

Ashley Wagoner joined as a 3rd year Neuroscience graduate student under the advisement of Dr. Steve Walker at WFU School of Medicine. Ashley was born and raised in Mooresville, NC and received her BS in Neuroscience from Furman University in 2011.  Ashley’s research project focuses on elucidating central, autonomic, and enteric nervous system mechanisms underlying cardiovascular and gastrointestinal dysfunction in pediatric populations. Ashley has been conducting two different research projects to study these mechanisms. Her first project aims to develop a model to study intrinsic synaptic function of the enteric nervous system to investigate neuronal implications of gastrointestinal motility disorders, such as chronic nausea and gastroesophageal reflux disease. She is presently characterizing induced pluripotent stem cells (iPSCs) from a patient population with a partial deletion of the synaptic protein Shank3 that is essential for formation of excitatory synapses.

Her additional project aims to further define the phenotype of children with chronic nausea and orthostatic intolerance (OI), a type of dysfunction of the autonomic nervous system (dysautonomia). Using head upright tilt table testing, Ashley has been able to characterize neurohumoral and autonomic profiles in this patient population, which provides validation of non-invasive testing for diagnosis and direct evidence for optimal treatment approaches in children with OI and GI symptoms. Ashley is currently evaluating potential brain mechanisms involved in OI using fMRI and magnetic resonance spectroscopy. 

Publications:

Wagoner AL, Shaltout HA, Fortunato JE, Diz DI. Distinct Neurohumoral Biomarker Profiles in Children with Hemodynamically-Define Orthostatic Intolerance. Am J Physiol Heart Circ Physiol 310: H416 –H425, 2016.

Wagoner AL, Shaltout HA, Diz DI, Fortunato JE. Orthostatically-Induced Nausea and Altered Neurohumoral Responses in Children with Chronic Nausea.  American Journal of Gastroenterology (under review)

Wagoner AL, Fortunato JE, Diz DI, Shaltout HA. 1H-Magnetic Resonance Spectroscopy Reveals Elevation of MyoInositol and other Markers of Inflammation in the Dorsal Medulla of Children with Orthostatic Intolerance. (in preparation, intended submission April 2016)

Poster Presentations:

Wagoner AL, Mack DL, McKee EE, Walker SJ. A Novel Cost-Effective Approach to Derivation of Induced Pluripotent Stem Cells from Epstein-Barr Virus Immortalized β-Lymphoblastoid Cell Lines. Wake Forest Institute for Regenerative Medicine Retreat, Pinehurst, NC, February 2016.

Wagoner AL, Mack DL, McKee EE, Walker SJ. A Novel Cost-Effective Approach to Derivation of Induced Pluripotent Stem Cells from Epstein-Barr Virus Immortalized β-Lymphoblastoid Cell Lines. NCTERMS Conference and Innovation Summit, Winston-Salem, NC, October 2015.

Wagoner AL, Mack DL, McKee EE, Walker SJ. Modeling Enteric Nervous System Function in children with Phelan McDermid Syndrome. NCTERMS Conference and Innovation Summit, Winston-Salem, NC, October 2015

Wagoner AL, Mack DL, McKee EE, Walker SJ. A Novel Cost-Effective Approach to Derivation of Induced Pluripotent Stem Cells from Epstein-Barr Virus Immortalized β-Lymphoblastoid Cell Lines. International Meeting for Autism Research, Salt Lake City, UT, May 2015.

Wagoner AL, Mack DL, McKee EE, Walker SJ. Modeling Enteric Nervous System Function in children with Phelan McDermid Syndrome. International Meeting for Autism Research, Salt Lake City, UT, May 2015.

Awards & Recognition:
Student Silver Award in Clinical Science - Wake Forest School of Medicine Division of Surgical Sciences Research Day (November 2015)

Travel Award Recipient - Wake Forest University Graduate School of Arts & Sciences Alumni Student Travel Award to travel to American Heart Association Council for Hypertension (Fall 2014 & 2015)

Travel Award Recipient to attend the International Meeting of Autism Research, Salt Lake City, Utah (May 2015)

Poster Award in Clinical Sciences, International Society for Hypertension New Investigator Symposium, San Francisco, CA (September 2014)

Second Place Overall Poster Award at Wake Forest Institute for Regenerative Medicine Annual Retreat, Poster Session, Pinehurst, NC (February 2015)

Community Outreach:

Teaching Assistant for Neuroscience Graduate Course in Neuroanatomy (Fall 2014 & 2015)

Gave graduate-level lecture on the visual system to PhD and masters students

Student Representative member of Admissions Committee for Program in Neuroscience at Wake Forest University Graduate School of Arts & Sciences (2012-2015)

Reviewed and interviewed applicants to PhD & Masters programs, organized recruitment events for 3 years

Editor of The Neurotransmitter, newsletter for Western NC Chapter of SfN (2012-16)

Editor and contributor - 9 newsletters from 2012-2016

Steering Committee Coordinator of Brain Awareness Council at WFU (2013-15)

Abstract preparation for Society for Neuroscience Education Outreach Symposium (2014-15)

Summer Research Mentor - WFIRM Summer Scholars Program (2014)

Mentor to 2 undergraduate students conducting neuronal stem cell projects over 10 weeks

Volunteer of Brain Awareness Council at WFU (2011-present)

Participated in Grad 709 Outreach Course (2012-2013) for graduate-level course credit

Volunteered 20+ hours in Winston-Salem/Forsyth County Schools (K-12) each year

Brain awareness week volunteer at local children’s museum and SciWorks museum each year

Participated in Brain Injury Association of NC Walk-N-Roll (2012), American Heart Association Heart Walk (2015), Walk To End Alzheimer’s (2015)

Additional Collaborations:

ThermoFisher Scientific : characterization and validation of the commercially available Human Pluripotent Stem Cell Taqman Scorecard Assay.

David Mack, PhD, Assistant Professor at University of Washington Institute for Stem Cell and Regenerative Medicine: further development of disease-in-a-dish nervous system model of Phelan-McDermid Syndrome  

On going projects:
hPSC Scorecard Assay Characterization- We are currently validating 6 iPSC lines in pluripotent and directed differentiation states in collaboration with ThermoFisher Scientific and Dr. David Mack. (expected completion by summer 2016)
 
Magnetic resonance spectroscopy and fMRI studies in children with orthostatic intolerance - complete data analysis and manuscript preparation. (expected completion by summer 2016)

Career:

Ashley successfully defended in April, 2016. She applied to a number of clinical research and postdoctoral positions. Following her clinical research and translational experience, Ashley accepted a Clinical Research Trainee Fellowship Position at Quintiles, the world’s largest provider of biopharmaceutical development and commercial outsourcing services in Research Triangle Park, NC. This position provides regulatory affairs and clinical monitoring experience focusing on therapeutic areas including cardiology, neurology, medical devices, and cancer. Using her clinical operations experience, she aims to obtain a role in strategic planning of clinical trials and drug development. She officially accepted the position with start date of June 1, 2016.


 Meet Our Pre-doctoral Fellows

Cycle 1 (09/01/2013 – 10/30/2015) 
JP McQuilling (Left) and Hannah Baker (Right)  


Hannah B. Baker   

Mentors: George Christ, PhD and Aaron Mohs, PhD

Hannah Baker is currently a 5th year graduate student under the advisement of Dr. George Christ at the Wake Forest-Virginia Tech School of Biomedical Engineering and Sciences. Hannah was born and raised in the Twin Cities of Minnesota and received her B.S. in Biomedical Engineering from the University of Rochester in 2010.

Hannah is involved in the study of tissue engineering/regenerative medicine solutions for functional reconstruction of volumetric muscle loss (VML) injuries. Her research is funded by a diversity of sources including internal (Innovation Award for development and commercialization of the tissue engineered muscle repair (TEMR) technology), and external DOD funding from the Armed Forces Institute of Regenerative Medicine (AFIRM). The TEMR technology is funded by AFIRM for a pilot clinical study on 5 patients undergoing secondary revision of cleft. Her project involves development of biomaterial and bioreactor strategies for functional restoration of surgically created VML injuries analogous to the permanent VML injuries suffered by civilians and wounded warriors. She is presently focused on the use of rodent models for finalizing the applicability of the TEMR technology for cleft lip.

Publications:

Baker HB, McQuilling JP, King NM. Ethical Considerations in Tissue Engineering Research: Case Studies in Translation. Methods 2016 Apr 15; 99:135-44

Benjamin T. Corona, Ph.D., Catherine L. Ward, Ph.D., Hannah B. Baker, Thomas J. Walters, Ph.D., George J. Christ, Ph.D. Implantation of in vitro tissue engineered muscle repair (TEMR) constructs and bladder acellular matrices (BAM) partially restore in vivo skeletal muscle function in a rat model of volumetric muscle loss (VML) injury. Tissue Engineering Part A. December, 2013.

Baker HB, McQuilling, JP, King, NM. Ethical Considerations in Tissue Engineering Research: Case Studies in Translation. Methods in Tissue Engineering, 2015 (in review)

Oral Presentations:

Baker, HB. (October 2013). Keratin hydrogels as a delivery system for the regenerative treatment of volumetric muscle loss. NCTERMS Conference, Winston-Salem, NC. 2013.

Baker, HB, Passipieri, JA, Bergman, C. (February 2014). Skeletal muscle tissue engineering and the RMCC. 2014 WFIRM Annual Retreat, Pinehurst, NC.

Baker, HB. (October 2014). Keratin hydrogels as a cell and growth factor delivery vehicle for treatment of volumetric muscle loss. BMES Conference, San Antonio, TX.

Baker, HB (December 2014). Growth Factor Loaded Keratin Hydrogels for Treatment of a Sheet-like VML Injury in Mice. TERMIS Americas, Washington, D.C.

Career:

Hannah successfully defended her thesis June 24th, 2015. Consistent with Hannah’s goal is to stay closely involved with translational science by continuing to pursue research endeavors in musculoskeletal regeneration and by becoming more involved with the associated regulatory processes and policy, she submitted applications to a variety of postdoctoral positions in tissue engineering research labs as well as to the American Institute for Medical and Biological Engineering Scholars Program. On March 17th, 2015 she accepted a research associate position at the University of Maryland under advisement of Dr. John P. Fisher in the Bioengineering Department. She will start August 3rd.

 

John P. McQuilling  

Mentors: Emmanuel Opara, PhD and Benjamin Harrison, PhD

JP McQuilling is a 5th year graduate student at the Wake Forest-Virginia Tech School of Biomedical Engineering and Sciences. Originally from Asheville North Carolina, JP received his undergraduate degree in Biomedical Engineering in 2010, and has been a member of Dr. Opara’s research team since then.
   
John’s research is focused on developing novel procedures and devices to further advance encapsulated islet cell technology as a treatment for type-1 diabetes. Specifically, John investigated the application of growth factors for improving angiogenesis for implanted islet grafts, applications of particulate oxygen generating substances for preventing hypoxic injury to encapsulated islets, alternative collagenase free methods for isolating islets, and developed new devices for the encapsulation of cells and islets.

Publications:

McQuilling JP, Sittadjody S, Pareta R, Pendergraft S, Clark CJ, Farney AC, Opara EC.  Retrieval of Microencapsulated Islet Grafts for Post-transplant Evaluation.  Methods Mol Biol 2017; 1479:157-171. 

McQuilling JP, Opara EC.  Methods for Incorporating Oxygen-Generating Biomaterials into Cell Culture and Microcapsule Systems. Methods Mol Biol 2017; 1479: 135-141

Baker HB, McQuilling JP, King NM. Ethical Considerations in Tissue Engineering Research: Case Studies in Translation. Methods 2016 Apr 15; 99:135-44

McQuilling JP, Goodwin SM, Pareta R, Klumpe HE, Patel HS, Lubischer JL, Loboa EG, Grossfeld, RM. Histamine Alters Cytoplasmic Calcium, Proliferation and Osteogenic Differentiation of Human Adipose-derived Stem Cells. Stem Cells and Development (in review) Opara EC. Islet Function within a multilayer microcapsule and efficacy of angiogenic protein delivery in an omentum pouch graft. Biomaterials and Biomedical Engineering. (2014) 1(1): 1-13.

Baker HB, McQuilling, JP, King, NM. Ethical Considerations in Tissue Engineering Research: Case Studies in Translation. Methods in Tissue Engineering, 2015 (in review)  

Book Chapters:

McQuilling JP, Sivanandane S, Pareta R, Pendergraft S, Clark CJ, Farney AC, Opara EC. Retrieval of microencapsulated islets for post-transplant evaluation. In: Cell Microencapsulation: Methods and Protocols (Methods in Molecular Biology) Opara, EC. Humana Press. 2015; (in Press).

Oral Presentations:

McQuilling JP, Sittadjody S, Balaji S, Harrison B, Farney AC, Opara EC (September 2013). Particulate Oxygen Generating Substances (POGS) as Oxygen Source during Islet Isolation for improved viability and functionality. 2013 Biomedical Engineering Society Annual Meeting. Seattle, WA.

McQuilling JP, Sittadjody S, Harrison B, Farney AC, Opara EC (May 2014). Applications of Oxygen Generating Materials in a Bioartificial Pancreas. 13th Annual School of Biomedical Engineering and Science Research Symposium. Winston-Salem, NC.

McQuilling JP, Soland M, Almeida-Porada G, Opara EC (February 2014). Cell therapy for the treatment of Type 1 Diabetes. 2014 WFIRM Annual Retreat, Pinehurst, NC.

Poster Presentations:

McQuilling JP, Sivanadane S, Steinman J, Orlando G, Farney AC, Opara EM (Oct 2014). Successful Isolation of Human islets using a Collagenase free Osmotic Shock Method. 2014 Biomedical Engineering Society Annual Meeting. San Antonio, TX. 

McQuilling JP, Sittadjody S, Balaji S, Harrison B, Farney AC, Opara EC (Feb 2014). Particulate Oxygen Generating Substances (POGS) as Oxygen Source for Islet Isolation and Processing. 2014 WFIRM Annual Retreat, Pinehurst, NC.

Ngarande E, McQuilling JP, Opara EC (August 2014). Tissue Culture Techniques to Closely Mimic the Natural Microenvironment of Islet of Langerhans Cells. 2014 Summer Scholars Annual Research Day, Winston Salem, NC. (Graduate Student Mentor to University of Cape Town, S. Africa, summer research exchange student)

Career:

John successfully defended on Friday, April 24th, 2015. He applied to a number of research and development positions including: United Therapeutics in RTP, Cook Medical in Indiana, and a biomaterials postdoctoral position at the Naval Research Laboratory. On January 22nd, 2015, John accepted a position of “Senior Research Engineer” at NuTech Medical, in Birmingham Alabama. It is a research and development position which is focused on developing their allograft derived products from human amnion an amniotic fluid, as well as some cell based therapies using their amniotic fluid stem cells. He has officially accepted the position with official start date of April 6, 2015.


 

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