Sang Jin Lee, PhD
Sang Jin Lee, Ph.D., Associate Professor
Dr. Sang Jin Lee was born and raised in Korea. He received
his Ph.D. in Chemical Engineering at Hanyang University, Korea in 2003 and took
a postdoctoral fellowship in the Laboratories for Tissue Engineering and
Cellular Therapeutics at Harvard Medical School/Children’s Hospital Boston and
the Wake Forest Institute for Regenerative Medicine at Wake Forest School of
Medicine where he is currently a faculty member.
OF AREA OF INTEREST: Designing
3-D microenvironment for tissue engineering applications (bone, cartilage,
muscle, tendon, blood vessel, heart, & kidney); 3-D integrated organ
printing (IOP) system for fabricating complex, composite tissues or organs;
Interactions between biomaterials and cell/tissue; Drug/protein delivery
system; Development of enabling technologies for regenerative medicine (e.g.
bioreactor, bioimaging, etc.).
OF INTEREST: Biomaterials
have played an enormous role in virtually every application in tissue
engineering and regenerative medicine that support engineered tissues and
organs until repairing the function and structural maintenance. Dr. Lee’s
research efforts have focused on the design of 3-D biomaterial scaffolding
system for clinical translation. Dr. Lee’s current research projects are:
1. Smart biomaterial scaffolding
support the regenerative medicine and tissue engineering strategies. These
smart scaffolding systems combined with drug/protein delivery system,
nano/micro-scaled topographical feature, or hybrid materials that could
actively participate in functional tissue regeneration.
SJ, Liu J, Oh SH, Soker S, Atala A, Yoo JJ. Development of a composite vascular
scaffolding system that withstands physiological vascular conditions.
MR, Lee SJ, Stitzel JD, Atala A, Yoo JJ. Co-electrospun dual scaffolding system
with potential for muscle-tendon junction tissue engineering. Biomaterials.
SJ, Atala A. Scaffold technologies for controlling cell behavior in tissue
engineering. Biomed Mater. 2013;8(1):010201.
YJ, Kang HW, Lee SJ, Atala A, Yoo JJ. Bioprinting technology and its
applications. Eur J Cardiothorac Surg. 2014;46(3):342-8.
Merceron TK, Burt M, Seol Y-J, Kang H-W, Lee SJ,
Yoo JJ, and Atala A, A3-D bioprinted complex structure for engineering
muscle-tendon unit, Biofabrication, 2015;7:035003.
2. A noninvasive fluorescence-based
has been developed with a team of collaborators. Lee’s group used an invisible
near-infrared (NIR) fluorophore having excellent optical properties and high
physicochemical stability in serum and in the body, which enabled longitudinal
monitoring of scaffold degradation and cell behaviors in vivo.
SH, Lee JH, Hyun H, Ashitate Y, Park G, Robichaud K, Lunsford E, Lee SJ, Khang
G, Choi HS. Near-infrared fluorescence imaging for noninvasive trafficking of
scaffold degradation. Sci Rep. 2013;3:1198.
BM, Hofmann MC, Lu P, Xu Y, Rylander CG, Wang G, Sapoznik E, Criswell T, Lee
SJ, Soker S, Rylander MN. Dynamic, nondestructive imaging of a bioengineered
vascular graft endothelium. PLoS One. 2013;8(4):e61275.
EA, Hyun H, Kim SH, Lee JH, Park G, Ashitate Y, Choi J, Hong GH, Alyabyev S,
Lee SJ, Khang G, Henary M, Choi HS. Highly charged cyanine fluorophores for
trafficking scaffold degradation. Biomed Mater. 2013;8(1):014109.
3. Stem cell-based tissue regeneration has the potential to effectively
change the treatment of injured or damaged tissues/organs. Hence, current
research focuses on the potential uses of these stem cells and tissue
engineering strategies for translational applications.
S, Ko IK, Atala A, Yoo JJ, Lee SJ. Amniotic fluid-derived stem cells in
regenerative medicine research. Arch Pharm Res. 2012;35(2):271-80.
MT, Lee SJ, Gomes ME, Reis RL, Atala A, Yoo JJ. Amniotic fluid-derived stem
cells as a cell source for bone tissue engineering. Tissue Eng Part A.
AI, Rodrigues MT, Lee SJ, Atala A, Yoo JJ, Reis RL, Gomes ME. Understanding the
role of growth factors in modulating stem cell tenogenesis. PLoS One. 2013;8(12):e83734.
J, Jeong SY, Ju YM, Yoo JJ, Smith TL, Khang G, Lee SJ, Atala A. In vitro
osteogenic differentiation of human amniotic fluid-derived stem cells on a
poly(lactide-co-glycolide) (PLGA)-bladder submucosa matrix (BSM) composite
scaffold for bone tissue engineering. Biomed Mater. 2013;8(1):014107.
4. In situ tissue regeneration that is to take advantage of the
body’s own regenerating capacity by using the host's ability to mobilize
endogenous stem cells to the site of injury. Lee’s group has focused on development
of strategies for in situ tissue regeneration in terms of mechanism of host
cell recruitment, cell sourcing, cellular and molecular roles in cell
differentiation, navigational cues and niche signals, and a tissue-specific
smart biomaterial system from the perspective of regenerative medicine and
SJ, Van Dyke M, Atala A, Yoo JJ. Host cell mobilization for in situ tissue
regeneration. Rejuvenation Res. 2008;11(4):747-56.
IK, Ju YM, Chen T, Atala A, Yoo JJ, Lee SJ. Combined systemic and local
delivery of stem cell inducing/recruiting factors for in situ tissue
regeneration. FASEB J. 2012;26(1):158-68.
IK, Lee SJ, Atala A, Yoo JJ. In situ tissue regeneration through host stem cell
recruitment. Exp Mol Med. 2013 Nov 15;45:e57.
YM, Atala A, Yoo JJ, Lee SJ. In situ regeneration of skeletal muscle tissue
through host cell recruitment. Acta Biomater. 2014;10(10):4332-9.
number of patients suffering from diseases of the vascular system has resulted
in a greater demand for the development of functional
vascular substitutes to meet the clinical needs. Small-diameter (<5 mm)
vascular substitutes are especially needed for coronary artery bypass,
peripheral revascularization, and arteriovenous (A-V) fistula. Key challenges associated
with adequate functional outcome of vascular grafting remain unsolved.
Currently, my lab is developing a functional vascular scaffolding system by
combining autologous vascular cells with a natural and/or synthetic scaffold to
create an implantable vascular construct.
SJ, Yoo JJ, Lim GJ, Atala A, Stitzel J. In vitro evaluation of electrospun
nanofiber scaffolds for vascular graft application. J Biomed Mater Res A.
YM, Choi JS, Atala A, Yoo JJ, Lee SJ. Bilayered scaffold for engineering
cellularized blood vessels. Biomaterials. 2010;31(15):4313-21.
J, Yoo JJ, Atala A, Lee SJ. The effect of controlled release of PDGF-BB from
heparin-conjugated electrospun PCL/gelatin scaffolds on cellular bioactivity
and infiltration. Biomaterials. 2012;33(28):6709-20
H, Ju YM, Takahashi H, Williams DF, Yoo JJ, Lee SJ, Okano T, Atala A.
Engineered small diameter vascular grafts by combining cell sheet engineering
and electrospinning technology. Acta Biomater. 2015;16:14-22.