Brazil Courses

Courses - Brazilian Portuguese

POR 1310   Brazilian Culture
POR 1311   Elementary Portuguese I
POR 1312   Elementary Portuguese II
POR 2311   Intermediate Portuguese I

For information, contact Robert Anderson, x3452, andersonrn@wssu.edu.

2.1. Integrative Application of Biotechniques Used in Modern Day Biomedical Science

Integrative Application of Biotechniques
Used in Modern Day Biomedical Science

Course objective:

Current day medical research depends upon expertise in many areas of basic science that range from molecular biology to integrative physiology and pathophysiology. Students will receive didactic and hands-on training in several disciplines that are commonly used in the investigation of physiologic and pathophysiologic mechanisms of cardiovascular disease. The goal of this course is to equip students with in-depth knowledge of the disciplines of molecular biology, biochemistry, cellular physiology, imaging strategies, contemporary bioassays, whole animal physiology, and pharmacology. Students will be expected to apply the knowledge obtained in this course to the specific research projects they have been assigned. Successful completion of this course should enable students to translate specific disciplines to an integrative view of normal and abnormal physiology.

Topics:

Topic 1:  Immunoassays

1.1.      Elisa
1.2.      Radioimmunoassays

Objective:  This aspect of the course will focus on contemporary approaches and methods used for the measurement of bioactive substances in blood, cerebrospinal fluid, urine, and tissues. 

Topic 2:  "in vivo" physiological measurements

1.3.      Blood pressure
1.4.      Blood flow
1.5.      Cardiac function
1.6.      Electrocardiogram
1.7.      Whole nerve recording
1.8.      Ganglionic nerve transmission

Objective: Students will be taught to prepare animals for the measurement of physiologic variables to include blood pressure, blood flow, ECG, cardiac function, nerve activity, and ganglionic neurotransmission. This part of the course will emphasize the proper procedures for anesthetizing animals and surgery. Students will be introduced to the electronic equipment used for the measurement of these physiologic variables.

Topic 3: Receptor Binding (Diz)

1.9.    Radioisotopes
1.10.  Autoradiography
1.11.  Membrane Binding

Objective:  Students will be taught the theory upon which receptor binding is based.  Laboratory exercises will instruct the students in the proper use of radio-isotopes, preparation of tissue for receptor binding, and analyses of binding data.

Topic 4:  Molecular Biochemistry

1.12.  Real-Time PCR
1.13.  Gene Array
1.14.  Primer designing

Objective:  Students will be taught molecular biology and biochemistry that serve as the bases for polymerase chain reaction assays. In the laboratory session students will apply this knowledge and be introduced to design of oligonucleotide primers used in PCR.

Topic 5:  Peptide/Protein Characterization

1.15.  HPLC
1.16.  Western blots
1.17.  2D-electrophoresis
1.18.  Ultracentrifugation
1.19.  Mass spectrometry

Objective:  This aspect of the course will focus on biochemical methods used to identify and separate peptides and proteins in blood, cerebrospinal fluid, urine, and tissues.

Topic 6: Cell Identification

1.20.  Flow cytometry
1.21.  Histology and immunohistochemistry
1.22.  Confocal microscopy
1.23.  Electron microscopy

Objective:  Students will be exposed to a variety of methods and approaches used for identification of cells. The course will cover histological methods used to identify cells as well as substances (proteins and peptides) found in cells and their interstitial environment. The laboratory session will introduce students to light (standard transmission and confocal) and electron microscopy. Flow cytometry will be taught as a method used to separate cells based upon phenotypic characteristics of cells. Finally, students will be introduced to strategies for quantification and analysis of histological data.

Grading

The course grade will be based on completion of 6 reports on the various topics that will compromise 100% of the grade.

Quick Reference

Hypertension & Vascular Research Center
Debra I. Diz, PhD
Program Director

Phone: 336-716-2150
FAX: 336-716-2456

ddiz@wakehealth.edu

Janie Marshall

Phone: 336-716-1080

jmarshal@wakehealth.edu

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