Amanda Beauchamp, PhD

ABeauchamp

Postdoctoral Fellow
University of Minnesota Masonic Cancer Research Center

Email:  abeaucha@umn.edu

Education:
PhD Molecular Medicine and Translational Science 2007-2012
Wake Forest School of Medicine

BS (Biology), 2007, Campbell University

Advisor: Waldemar Debinski, M.D., Ph.D.: Surgical Sciences (BTCOE)

Current Research:

High-grade astrocytomas, specifically WHO grade IV glioblastoma multiforme (GBM) are the most common primary malignant brain tumor in adults. Despite the current standard of care (resection, chemotherapy, and radiation), GBM confers a poor prognosis with a median survival rate that has increased only by months in the past several decades.  Hence, there is a great need for novel therapies that target GBM cells while leaving normal brain tissue unharmed. One such proposed target is the EphA2 receptor, which our laboratory has shown to be overexpressed in GBM tumors and cell lines, and nearly absent in normal tissue. Activation of EphA2 by its ligand, ephrinA1, induces phosphorylation and degradation of the EphA2 protein, and subsequently leads to decreased tumorigenic properties such as invasion, migration, and anchorage independent growth.

Until recently, ephrinA1 was believed to be able to activate EphA2 only when in a dimeric or oligomeric state, or when anchored to the membrane of an adjacent cell. However, we have recently reported that a monomeric form of ephrinA1 is released into the media of ephrinA1-transfected U-251 (GBM cell line) cells and that it can act in a paracrine fashion as a soluble monomer and functional ligand for EphA2. Monomeric ephrinA1 causes receptor phosphorylation and downregulation, and decreases the invasiveness and migration of carcinogenic cells.  While tumor-suppressing abilities of monomeric ephrinA1 have been established, not much is currently known about the mechanism of release of ephrinA1 from the cell membrane, or the downstream signalling pathways stimulated by its binding to EphA2. My project encompasses understanding the mechanism of ephrinA1 cleavage from the cell membrane, downstream signalling as a result of monomeric ephrinA1 activation of the EphA2 receptor, and potential cytotoxin therapies using the monomeric ephrinA1 ligand to target EphA2-overexpressing GBM cells.

Professional Associations:

2008-present  Society for Neurooncology
2010-present AACR  

Awards:

Silver Award- 19th Surgical Sciences Research Day, November 17, 2011.
Basic Science – Students category.  “EphrinA1 is Released into the Extracellular Space of Cancer Cells by Proteolytic Cleavage by MMP-1, -2, -9, and -13 to Produce EphrinA1-175, -177, and -178 Isoforms”.

Abstracts/Scientific Exhibits:

Amanda Beauchamp, Denise M. Gibo, Jill Wykosky, Akiva Mintz and Waldemar Debinski. “Elucidation of the Release and Function of Monomeric ephrinA1 in Glioblastoma Multiforme.”  American Association of Cancer Research Conference. Orlando, Florida. April 1-6, 2011.

Amanda Beauchamp, Denise M. Gibo, Jill Wykosky and Waldemar Debinski. “Elucidation of the Release and Function of Monomeric ephrinA1 in Glioblastoma Multiforme.”  Society for Neuro-oncology Conference. Montreal, QC Canada. November 19-21, 2010.

Van Nguyen, L. Tiara Murphy, Amanda Beauchamp, Charlotte K. Hollingsworth, Waldemar Debinski, and Akiva Mintz. “Targeting the EphA2 Biomarker in Malignant Glioma.”  Eph/ephrins and Cancer Conference, Winston-Salem, NC 2010

Amanda Beauchamp, Denise M. Gibo, Jill Wykosky, and Waldemar Debinski. “Studies on the Release of Monomeric EphrinA1 from Cancer Cells.” Eph/ephrins and Cancer Conference, Winston-Salem, NC 2010

Amanda Beauchamp, Denise M. Gibo, Jill Wykosky, and Waldemar Debinski. “Studies on the Release and Function of Monomeric EphrinA1 in Glioblastoma Multiforme.” Surgical Sciences Research Day. Wake Forest University. November 19, 2009.

Amanda Beauchamp, Denise M. Gibo, Jill Wykosky, and Waldemar Debinski. “Studies on the Release and Function of Monomeric EphrinA1 in Glioblastoma Multiforme.” Society for Neuro-oncology Conference. New Orleans, LA. October 22-24, 2009.

Amanda Beauchamp, Denise M. Gibo, Jill Wykosky, and Waldemar Debinski. “Investigation of the Mechanism and Form of EphrinA1 Released from Cancer Cells.” Wake Forest University Graduate Student Research Day. 2009.

Amanda Beauchamp, Denise M. Gibo, Jill Wykosky, Peter Dickinson, and Waldemar Debinski. “The Presence of Ephs and ephrins in Canine Brain Tumors.” Eph/ephrins and Cancer Conference, Winston-Salem, NC 2008

 

Quick Reference

Contact Information
Molecular Medicine and Translational Science Graduate Program

Office 336-713-4259

Kay Collare

336-713-4259

kcollare@wakehealth.edu

Find A Doctor Ways to Give
Last Updated: 12-03-2014
USNWR 2013-2014Magnet Hospital RecognitionConsumer Choice2014 Best DoctorsJoint Commission Report

Disclaimer: The information on this website is for general informational purposes only and SHOULD NOT be relied upon as a substitute for sound professional medical advice, evaluation or care from your physician or other qualified health care provider.