Bladder Research Center

Research

Cells for Web

A primary goal of the center is to develop a mouse model to gain understanding of the mechanisms of bladder regeneration. Scientists are using new imaging technologies to identify the sources of cells that are responsible for rapid bladder wall regeneration.  

A series of Supplement Awards, for additional work, has been received by our Bladder Research Center. A list is provided below: 

BLADDER IMAGING: Administrative Supplement (WFIRM) The goal of this supplement is two-fold: 1) we will adapt our existing multispectral in vitro IHC capabilities to an in vivo approach that will permit serial imaging of specific transplanted cells in the bladder over the entire time course of the regenerative response (F. Marini, PhD, PI), and 2) we will use novel blood pool MRI contrast agents to permit an extensive investigation of angiogenesis during the time course of bladder regeneration (A. Mohs, PhD, PI). Taken together, these two studies will provide an unprecedented level of mechanistic insight concerning both key spatiotemporal contributions of various transplanted cell types to bladder regeneration, as well as providing the first description we are aware of, concerning the “real time” process of angiogenesis during mammalian organ regeneration.

BLADDER STEM CELL TRACKING: Administrative Supplement to Cathy Mendelsohn, PhD, Columbia University This collaboration allows both groups to utilize murine subtotal cystectomy/transplant models for harnessing the power of mouse genetics and further explore cellular and molecular mechanisms of bladder regeneration. Specifically, the work will permit lineage tracing of urothelial progenitor cell participation in the complete functional regeneration observed in the murine bladder following 60% subtotal cystectomy. The first model to be tested will be the Upk3aGCE (UpK3a: uroplakin 3) transgenic cherry line from Dr. Mendelsohn’s lab.

TRANSGENIC RAT DEVELOPMENT: Administrative Supplement to Chuck Bierberich, PhD, University of Maryland, Baltimore The goal of this collaboration is to develop a novel transgenic RAT model to elucidate the role of UpkII+ (uroplalkin) cells during rat bladder regeneration, and this transgenic rat will also bolster the knowledge base by allowing the GUDMAP project members to have access to a rat model in addition to the current mouse models. Moreover, future generated transgenic rats can benefit from the use of UpkII-restricted rtTA expression to specifically target gene activity to the bladder. In addition, parallel development of transgenic rat lines seems a logical extension of ongoing mouse molecular genetics.  

Read about our latest bladder regeneration research:

"Age-Related Alterations in Regeneration of the Urinary Bladder after Subtotal Cystectomy." 
View the Manuscript  

“Characterization of the early proliferative response of the rodent bladder to subtotal cystectomy: A unique model of mammalian organ regeneration.”
View the Manuscript
Read a News Release

“Early stages of in situ bladder regeneration in a rodent model.” Burmeister D, Aboushwareb T, Tan J, Link K, Andersson KE, Christ G. Tissue Eng Part A. 2010 Aug;16(8):2541-51
View the Manuscript

 

Quick Reference

Institute for Regenerative Medicine

Phone 336-713-7293
Fax 336-713-7290

Location
Richard H. Dean Biomedical Building
391 Technology Way
Winston-Salem, NC 27101
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Last Updated: 01-29-2014
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