Transgenic Mouse Core Facility

Mission

The Transgenic Mouse Core Facility provides investigators at Wake Forest University Health Sciences (WFUHS) access to state-of-the-art technologies for the generation of genetically-engineered mouse lines.

Services

• Consultation with the Core Director regarding the strategy for production and testing of the transgenic and gene targeting constructs 
• Pronuclear microinjection of DNA into fertilized mouse embryos for the production of transgenic mouse lines
• Gene targeting in mouse embryonic stem (ES) cells
• Injection of gene-targeted ES cells into mouse blastocysts to produce chimeric mice for the creation of Knock-out/Knock-in mouse lines
• Embryo rederivation for eradication of horizontally-transmitted, post-implantation, and vertically transmitted pathogens
• Mouse genotyping from tail clips, using polymerase chain reaction (PCR) procedures 

Details

:: About Transgenic Mice ::  About Knock-out/Knock-in Mice ::  About Embryo Rederivation :: About Mouse Genotyping Service ::  Procedure Request Forms ::

Consultation with the Core Director regarding the strategy for production and testing of the transgenic and gene targeting constructsPronuclear microinjection of DNA into fertilized mouse embryos for the production of transgenic mouse linesGene targeting in mouse embryonic stem (ES) cellsInjection of gene-targeted ES cells into mouse blastocysts to produce chimeric mice for the creation of Knock-out/Knock-in mouse linesEmbryo rederivation for eradication of horizontally-transmitted, post-implantation, and vertically transmitted pathogens Mouse genotyping from tail clips, using polymerase chain reaction (PCR) procedures.

Staff

• Liqing Yu, MD PhD
  Director, Transgenic Mouse Core Facility
    
• Lihong Shi, MD
  Transgenic Mouse Specialist
  
• Feng Guo, MD
  Laboratory Manager

Additional Information

These Cell Migration Consortium/Nature Publishing Group websites provide useful background or resource material for those unfamiliar with the procedures:

• Beginners with Transgenic or Knockout Mice, start here.
• For a guide to the basics of preparing Gene Targeting Constructs, go here.

Note: 

If you cannot make your own gene targeting constructs, there are companies that will make gene targeting constructs for a fee. The Transgenic Mouse Core staff have not had direct experience with any of these companies, but would be happy to provide investigators with their names. Call Dr. Liqing Yu at 716-0920 for more information.

ES Cell Lines Available for Generating Some Knockout Mice

Some knockout mice can be made without the investigator having to generate the targeting constructs or the targeted mouse embryonic cells.æ Please check the following links to see if the gene of interest has been targeted in the mouse embryonic stem cells or even in mice.

The International Mouse Knockout Consortium: 

National Institutes of Health Knockout Mouse Project (KOMP)

European Conditional Mouse Mutagenesis Program (EUCOMM)

North American Conditional Mouse Mutagenesis Project (NorCOMM)

Other Useful Links:

• International Gene Trap Consortium
• The Mouse Genome Informatics
• Mutant Mouse Regional Resource Centers
• International Mouse Strain Resource (IMSR)
• Texas Institute for Genomic Medicine (TIGM)

Call the Transgenic Mouse Core Facility's director, Dr. Liqing Yu at 716-0920 for more information.

Internal Advisory Committee

Richard St. Clair, PhD
Chair, Pathology - Lipid Sciences

Paul Dawson, PhD  
Internal Medicine - Gastroenterology, Pathology - Lipid Sciences

John Parks, PhD  
Pathology - Lipid Sciences

Larry Rudel, PhD 
Pathology - Lipid Sciences

Sara Jones, PhD  
Physiology/Pharmacology