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Leslie B. Poole, Ph.D.Wake Forest School of Medicine

Leslie B. Poole, Ph.D.


Clinical Interests

Contact Information

Academic: 336-716-6711 | Department: 336-716-6711


Education & Training

  • B.A., Wake Forest University, 1980
  • Ph.D., Wake Forest University, 1988
  • Fellowship, University of Maryland-College, 1991


  • Protein Society
  • Society Free Radical Biology
  • Am Chemical Society
  • Am Soc Of Biochem & Mol Biolog
  • Am Soc of Microbiology
Leslie B. Poole, Ph.D.

Leslie B. Poole, Ph.D.

Professor, Biochemistry

Research Interests

Peroxiredoxins; Cysteine; Sulfenic Acids; Proteins; Oxidation-Reduction
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Contact Information

Academic: 336-716-6711 | Department: 336-716-6711


Recent Publications

Experimentally dissecting the origins of peroxiredoxin catalysis. Nelson KJ, Perkins A, Van Swearingen AED, Hartman S, Brereton AE, Parsonage D, Salsbury FR Jr, Karplus PA, Poole LB.. Antioxid Redox Signal. 2018;28(7):521-536.

Mitochondria-targeted probes for imaging protein sulfenylation. Holmila RJ, Vance SA, Chen X, Wu H, Shukla K, Bharadwaj MS, Mims J, Wary Z, Marrs G, Singh R, Molina AJ, Poole LB, King SB, Furdui CM.. Sci Rep. 2018;8(1):6635.

Oxidative stress-induced sulfenylation of SIRT6 is associated with enhanced NF-kappa B signaling in human chondrocytes [abstract]. Collins JA, Diekman BO, DeFoor MT, Wood ST, Bolduc JA, Nelson KJ, Chubinskaya S, Poole LB, Furdui CM, Loeser RF.. Osteoarthritis Cartilage. 2018;26(Suppl 1):S44.

Novel hyperoxidation resistance motifs in 2-Cys peroxiredoxins. Bolduc JA, Nelson KJ, Haynes AC, Lee J, Reisz JA, Graff AH, Clodfelter JE, Parsonage D, Poole LB, Furdui CM, Lowther WT.. J Biol Chem. 2018;293(30):11901-12.

Differential kinetics of two-cysteine peroxiredoxin disulfide formation reveal a novel model for peroxide sensing. Portillo-Ledesma S, Randall LM, Parsonage D, Dalla Rizza J, Karplus PA, Poole LB, Denicola A, Ferrer-Sueta G.. Biochemistry. 2018;57(24):3416-3424.

An atlas of peroxiredoxins created using an active site profile-based approach to functionally relevant clustering of proteins. Harper AF, Leuthaeuser JB, Babbitt PC, Morris JH, Ferrin TE, Poole LB, Fetrow JS.. PLoS Comput Biol. 2017;13(2):e1005284.

The SAMHD1 dNTP triphosphohydrolase is controlled by a redox switch. Mauney CH, Rogers LC, Harris RS, Daniel LW, Devarie-Baez NO, Wu H, Furdui CM, Poole LB, Perrino FW, Hollis T.. Antioxid Redox Signal. 2017;27(16):1317-1331.

Endogenous, regulatory cysteine sulfenylation of ERK kinases in response to proliferative signals. Keyes JD, Parsonage D, Yammani RD, Rogers LC, Kesty C, Furdui CM, Nelson KJ, Poole LB.. Free Radic Biol Med. 2017;112():534-543.

Discovery of heteroaromatic sulfones as a new class of biologically compatible thiol-selective reagents. Chen X, Wu H, Park CM, Poole TH, Keceli G, Devarie-Baez NO, Tsang AW, Lowther WT, Poole LB, King SB, Xian M, Furdui CM.. ACS Chem Biol. 2017;12(8):2201-2208.

Differential peroxiredoxin hyperoxidation regulates map kinase signaling in human articular chondrocytes [abstract]. Collins JA, Nelson KJ, Poole LB, Furdui CM, Loeser RF.. Osteoarthritis Cartilage. 2017;25(Suppl 1):S164-S165.

The oxidative state of cysteine thiol 144 regulates the SIRT6 glucose homeostat. Long D, Wu H, Tsang AW, Poole LB, Yoza BK, Wang X, Vachharajani V, Furdui CM, McCall CE.. Sci Rep. 2017;7(1):11005.

Impact of redox modifications on ERK2 substrate phosphorylation [abstract]. Keyes JD, Postiglione A, Patwardhan S, Smith-Pearson P, Parsonage D, Newman RH, Poole LB.. Free Radic Biol Med. 2017;112(Suppl 1):193-194.

Cysteine-mediated redox regulation of cell signaling in chondrocytes stimulated with fibronectin fragments. Wood ST, Long DL, Reisz JA, Yammani RR, Burke EA, Klomsiri C, Poole LB, Furdui CM, Loeser RF.. Arthritis Rheumatol. 2016;68(1):117-126.

Backbone chemical shift assignments for Xanthomonas campestris peroxiredoxin Q in the reduced and oxidized states: a dramatic change in backbone dynamics. Buchko GW, Perkins A, Parsonage D, Poole LB, Karplus PA.. Biomol NMR Assign. 2016;10(1):57-61.

Structural changes upon peroxynitrite-mediated nitration of peroxiredoxin 2; nitrated Prx2 resembles its disulfide-oxidized form. Randall L, Manta B, Nelson KJ, Santos J, Poole LB, Denicola A.. Arch Biochem Biophys. 2016;590():101-108.

Distribution and features of the six classes of peroxiredoxins. Poole LB, Nelson KJ.. Mol Cells. 2016;39(1):53-59.

Oxidative stress promotes peroxiredoxin hyperoxidation and attenuates pro-survival signalling in aging chondrocytes. Collins JA, Wood ST, Nelson KJ, Rowe MA, Carlson CS, Chubinskaya S, Poole LB, Furdui CM, Loeser RF.. J Biol Chem. 2016;291(13):6641-6654.

X-ray structures of thioredoxin and thioredoxin reductase from Entamoeba histolytica and prevailing hypothesis of the mechanism of Auranofin action. Parsonage D, Sheng F, Hirata K, Debnath A, McKerrow JH, Reed SL, Abagyan R, Poole LB, Podust LM.. J Struct Biol. 2016;194(2):180-190.

The level of reactive oxygen species differentially regulates peroxiredoxin oxidation and mitogen-activated protein kinase signaling in human chondrocytes [abstract]. Collins JA, Wood ST, Poole L, Furdui C, Loeser R.. Osteoarthritis Cartilage. 2016;24(Suppl):S179-S180.

Peroxiredoxin catalysis at atomic resolution. Perkins A, Parsonage D, Nelson KJ, Ogba OM, Cheong PH, Poole LB, Karplus PA.. Structure. 2016;24(10):1668-1678.

The basics of thiols and cysteines in redox biology and chemistry. Poole LB.. Free Radic Biol Med. 2015;80():148-157.

Dissecting peroxiredoxin catalysis: separating binding, peroxidation, and resolution for a bacterial AhpC. Parsonage D, Nelson KJ, Ferrer-Sueta G, Alley S, Karplus PA, Furdui CM, Poole LB.. Biochemistry. 2015;54(7):1567-1575.

Introduction: what we do and do not know regarding redox processes of thiols in signaling pathways. Poole LB, Schoneich C.. Free Radic Biol Med. 2015;80():145-147.

Mitochondrial reactive oxygen species (ROS) promote hyperoxidation of peroxiredoxins and inhibit pro-survival IGF-1 signaling in human articular chondrocytes [abstract]. Collins JA, Poole L, Furdui C, Nelson K, Loeser R.. Osteoarthritis Cartilage. 2015;23(Suppl 2):A32-A33.

Peroxiredoxins: guardians against oxidative stress and modulators of peroxide signaling. Perkins A, Nelson KJ, Parsonage D, Poole LB, Karplus PA.. Trends Biochem Sci. 2015;40(8):435-445.

Cysteine-mediated redox regulation of cell signaling in chondrocytes stimulated with fibronectin fragments [abstract]. Wood ST, Long D, Reisz J, Yammani R, Burke E, Klomsiri C, Poole L, Furdui C, Loeser R.. Osteoarthritis Cartilage. 2015;23(Suppl 2):A65-A66.

Au-ACRAMTU-PEt3 alters redox balance to inhibit T cell proliferation and function. Langston PK, Yang M, Bierbach U, Parsonage D, Poole LB, Price MJ, Grayson JM.. J Immunol. 2015;195(5):1984-1994.

Relation of pre-anthracycline serum bilirubin levels to left ventricular ejection fraction after chemotherapy. Vera T, D'Agostino RB Jr, Jordan JH, Whitlock MC, Melendez GC, Lamar ZS, Porosnicu M, Bonkovsky HL, Poole LB, Hundley WG.. Am J Cardiol. 2015;116(11):1752-1755.

Chemical approaches to detect and analyze protein sulfenic acids. Furdui CM, Poole LB.. Mass Spectrom Rev. 2014;33(2):126-146.

Endosomal H2O2 production leads to localized cysteine sulfenic acid formation on proteins during lysophosphatidic acid-mediated cell signaling. Klomsiri C, Rogers LC, Soito L, McCauley AK, King SB, Nelson KJ, Poole LB, Daniel LW.. Free Radic Biol Med. 2014;71():49-60.

Strained cycloalkynes as new protein sulfenic acid traps. Poole TH, Reisz JA, Zhao W, Poole LB, Furdui CM, King SB.. J Am Chem Soc. 2014;136(17):6167-6170.

Nitration transforms a sensitive peroxiredoxin 2 into a more active and robust peroxidase. Randall LM, Manta B, Hugo M, Gil M, Batthyany C, Trujillo M, Poole LB, Denicola A.. J Biol Chem. 2014;289(22):15536-43.

Evaluating peroxiredoxin sensitivity toward inactivation by peroxide substrates. Nelson KJ, Parsonage D, Karplus PA, Poole LB.. Methods Enzymol. 2013;527():21-40.

Sulfenic acids and peroxiredoxins in oxidant defense. Poole LB, Nelson KJ, Karplus PA. In: Jakob U, Reichmann D, eds. Oxidative stress and redox regulation. Dordrecht: Springer;2013: 85-118.

Modulation of signaling proteins by reversible cysteine modification [abstract]. Keyes JD, Nelson K, Parsonage D, Daniel L, Furdui C, Poole L.. FASEB J. 2013;27():993.3.

D-alanine modification of a protease-susceptible outer membrane component by the Bordetella pertussis dra locus promotes resistance to antimicrobial peptides and polymorphonuclear leukocyte-mediated killing. Taneja NK, Ganguly T, Bakaletz LO, Nelson KJ, Dubey P, Poole LB, Deora R.. J Bacteriol. 2013;195(22):5102-5111.

The sensitive balance between the fully folded and locally unfolded conformations of a model peroxiredoxin. Perkins A, Nelson KJ, Williams JR, Parsonage D, Poole LB, Karplus PA.. Biochemistry. 2013;52(48):8708-8721.

Thiol-blocking electrophiles interfere with labeling and detection of protein sulfenic acids. Reisz JA, Bechtold E, King SB, Poole LB, Furdui CM.. FEBS J. 2013;280(23):6150-6161.

Sulfenic acid-reactive compounds and their methods of synthesis. Poole LB, King SB, inventors; Wake Forest University Health Sciences, assignee.. United States patent US 8,486,642. 2013 July 16. 2013;():.

Peroxiredoxins as molecular triage agents, sacrificing themselves to enhance cell survival during a peroxide attack [comment]. Karplus PA, Poole LB.. Mol Cell. 2012;45(3):275-278.

A simple and effective strategy for labeling cysteine sulfenic acid in proteins by utilization of beta-ketoesters as cleavable probes. Qian J, Wani R, Klomsiri C, Poole LB, Tsang AW, Furdui CM.. Chem Commun (Camb). 2012;48(34):4091-4093.

Structural and electrostatic asymmetry at the active site in typical and atypical peroxiredoxin dimers. Salsbury FR Jr, Yuan Y, Knaggs MH, Poole LB, Fetrow JS.. J Phys Chem B. 2012;116(23):6832-6843.

A new family of membrane electron transporters and its substrates, including a new cell envelope peroxiredoxin, reveal a broadened reductive capacity of the oxidative bacterial cell envelope. Cho S-H, Parsonage D, Thurston C, Dutton RJ, Poole LB, Collet J-F, Beckwith J.. MBio. 2012;3(2):e00291-11.

A high-throughput drug screen for Entamoeba histolytica identifies a new lead and target. Debnath A, Parsonage D, Andrade RM, He C, Cobo ER, Hirata K, Chen S, Garcia-Rivera G, Orozco E, Martinez MB, Gunatilleke SS, Barrios AM, Arkin MR, Poole LB, McKerrow JH, Reed SL.. Nat Med. 2012;18(6):956-960.

Peroxiredoxins in parasites. Gretes MC, Poole LB, Karplus PA.. Antioxid Redox Signal. 2012;17(4):608-633.

Electrostatics of cysteine residues in proteins: parameterization and validation of a simple model. Salsbury FR Jr, Poole LB, Fetrow JS.. Proteins. 2012;80(11):2583-2591.

The reversible formation of cysteine sulfenic acid promotes B-cell activation and proliferation. Crump KE, Juneau DG, Poole LB, Haas KM, Grayson JM.. Eur J Immunol. 2012;42(8):2152-2164.

Mapping the active site helix-to-strand conversion of CxxxxC peroxiredoxin Q enzymes. Perkins A, Gretes MC, Nelson KJ, Poole LB, Karplus PA.. Biochemistry. 2012;51(38):7638-7650.

Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2: reaction intermediates revealed by crystallography and mass spectrometry. Lang BS, Gorren ACF, Oberdorfer G, Wenzl MV, Furdui CM, Poole LB, Mayer B, Gruber K.. J Biol Chem. 2012;287(45):38124-34.

Localized hydrogen peroxide-dependent cysteine oxidation is required for lysophosphatidic acid signaling in ovarian and prostate cancer cells [abstract]. Daniel LW, Klomsiri C, Rogers LC, Nelson KJ, Soito L, King SB, Poole LB.. Free Radic Biol Med. 2012;53(Suppl 2):S33.

All Publications

For a listing of recent publications, refer to PubMed, a service provided by the National Library of Medicine.

For a list of earlier publications, visit the Carpenter Library Publication Search.

Professor, Biochemistry

Clinical Interests

Leslie B. Poole, Ph.D.

Leslie B. Poole, Ph.D.

Professor, Biochemistry

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