Polymerase Chain Reaction Laboratory

Polymerase Chain Reaction (PCR)-based technology allows detection of DNA or RNA sequences that may be specific for certain microorganisms, genes, or gene products. Currently the PCR lab offers the following testing:

  • BCR-ABL quantitative, p120 major breakpoint
  • BCR-ABL quantitative, p190 minor breakpoint
  • BRAF mutational analysis
  • EGFR mutational analysis
  • Human papilloma virus (HPV), 14 high risk types including HPV types 16 and 18
  • Immunoglobulin heavy chain gene rearrangement
  • KRAS mutational analysis
  • PML-RARa, quantitative, bcr 1 (long) and bcr 3 (short) forms
  • T-cell receptor gamma gene rearrangement

All specimens submitted for analysis must be accompanied by a requisition form.

Samples for PCR may be submitted in several ways:

  • Paraffin embedded tissue, on glass slides
  • Paraffin block
  • Blood and bone marrow should be collected in EDTA only. Samples for PCR testing from body fluids require a dedicated sample. Tubes that have been opened and samples for other tests will be rejected (at the director's discretion).

Normal turnaround time is estimated to be 3-5 days depending upon the day the sample is received.

Please contact Dr. Stacey O'Neill for any PCR related questions.